Evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed)
Objective: To compared seed culture of hemorrhagic septicemia (HS) bacteria which was used to produce vaccine for its antibody induction efficiency before and after passaging in natural host (calf) using laboratory animals. Methods: Serial dilution of virulent bacteria was injected in to mice which...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:c50f08d79943413c9d5c12bb32a3379d 2023-05-15T15:13:17+02:00 Evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed) Maheswarappa Gowrakkal M Chandrashekar Shankarappa Bhajantri Jaykumar Satav GC Chandakala Asha Mayanna SM Byregowda C Renukaprasad 2014-05-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C554 https://doaj.org/article/c50f08d79943413c9d5c12bb32a3379d EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115302793 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C554 https://doaj.org/article/c50f08d79943413c9d5c12bb32a3379d Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S263-S267 (2014) Hemorrhagic septicemia Laboratory animals Natural host LD50 values Annual vaccination Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C554 2022-12-31T15:41:47Z Objective: To compared seed culture of hemorrhagic septicemia (HS) bacteria which was used to produce vaccine for its antibody induction efficiency before and after passaging in natural host (calf) using laboratory animals. Methods: Serial dilution of virulent bacteria was injected in to mice which were immunized with HS vaccine which was obtained from seed bacteria before and after back passaged in calf. Ratio of survived and dead was calculated by Reed-Meunch hypothesis and the LD50 value for each vaccine trial groups were calculated. Results: The immunological study revealed that vaccine prepared from back passaged seed culture showed greater improvement in its immunopotency than seed vaccine (before back passage). Around 200 mice were used to study the immuno efficiency of vaccine. Each mouse was from the same source, which were free from the Pastuerella infection previous to expose to trial infection. The same broth culture of HS was used to induce infection in mice in both trials (vaccine before back passage and vaccine after back passage). The 0.2 mL of broth dilution from 10−1 to 10−10 was used, as dilution increases, death rate decreases. It indicates the minimum load of bacterium is required to induced infection. Conclusions: Obtained results revealed that back passaged vaccine seed HS bacteria in its natural host had provided better immune efficiency to the culture than laboratory stock culture, and this findings recommended that regular annual back passage was mandatory for the vaccine seed culture of Pastuerella multocida bacteria for better establishment of immune potent vaccines. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 S263 S267 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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English |
topic |
Hemorrhagic septicemia Laboratory animals Natural host LD50 values Annual vaccination Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Hemorrhagic septicemia Laboratory animals Natural host LD50 values Annual vaccination Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Maheswarappa Gowrakkal M Chandrashekar Shankarappa Bhajantri Jaykumar Satav GC Chandakala Asha Mayanna SM Byregowda C Renukaprasad Evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed) |
topic_facet |
Hemorrhagic septicemia Laboratory animals Natural host LD50 values Annual vaccination Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To compared seed culture of hemorrhagic septicemia (HS) bacteria which was used to produce vaccine for its antibody induction efficiency before and after passaging in natural host (calf) using laboratory animals. Methods: Serial dilution of virulent bacteria was injected in to mice which were immunized with HS vaccine which was obtained from seed bacteria before and after back passaged in calf. Ratio of survived and dead was calculated by Reed-Meunch hypothesis and the LD50 value for each vaccine trial groups were calculated. Results: The immunological study revealed that vaccine prepared from back passaged seed culture showed greater improvement in its immunopotency than seed vaccine (before back passage). Around 200 mice were used to study the immuno efficiency of vaccine. Each mouse was from the same source, which were free from the Pastuerella infection previous to expose to trial infection. The same broth culture of HS was used to induce infection in mice in both trials (vaccine before back passage and vaccine after back passage). The 0.2 mL of broth dilution from 10−1 to 10−10 was used, as dilution increases, death rate decreases. It indicates the minimum load of bacterium is required to induced infection. Conclusions: Obtained results revealed that back passaged vaccine seed HS bacteria in its natural host had provided better immune efficiency to the culture than laboratory stock culture, and this findings recommended that regular annual back passage was mandatory for the vaccine seed culture of Pastuerella multocida bacteria for better establishment of immune potent vaccines. |
format |
Article in Journal/Newspaper |
author |
Maheswarappa Gowrakkal M Chandrashekar Shankarappa Bhajantri Jaykumar Satav GC Chandakala Asha Mayanna SM Byregowda C Renukaprasad |
author_facet |
Maheswarappa Gowrakkal M Chandrashekar Shankarappa Bhajantri Jaykumar Satav GC Chandakala Asha Mayanna SM Byregowda C Renukaprasad |
author_sort |
Maheswarappa Gowrakkal |
title |
Evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed) |
title_short |
Evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed) |
title_full |
Evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed) |
title_fullStr |
Evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed) |
title_full_unstemmed |
Evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed) |
title_sort |
evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed) |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C554 https://doaj.org/article/c50f08d79943413c9d5c12bb32a3379d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S263-S267 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115302793 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C554 https://doaj.org/article/c50f08d79943413c9d5c12bb32a3379d |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C554 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_start_page |
S263 |
op_container_end_page |
S267 |
_version_ |
1766343852512247808 |