Molecular cloning and characterization of a novel freezing-inducible DREB1/CBF transcription factor gene in boreal plant Iceland poppy (Papaver nudicaule)
Abstract DREB1 of the AP2/ERF superfamily plays a key role in the regulation of plant response to low temperatures. In this study, a novel DREB1/CBF transcription factor, PnDREB1, was isolated from Iceland poppy (Papaver nudicaule), a plant adaptive to low temperature environments. It is homologous...
Published in: | Genetics and Molecular Biology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Sociedade Brasileira de Genética
|
Subjects: | |
Online Access: | https://doi.org/10.1590/1678-4685-GMB-2015-0228 https://doaj.org/article/c4eb5f4b7dda40f999e8cb9b615e9a39 |
id |
ftdoajarticles:oai:doaj.org/article:c4eb5f4b7dda40f999e8cb9b615e9a39 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:c4eb5f4b7dda40f999e8cb9b615e9a39 2023-05-15T16:46:15+02:00 Molecular cloning and characterization of a novel freezing-inducible DREB1/CBF transcription factor gene in boreal plant Iceland poppy (Papaver nudicaule) Zhuo Huang Jiao He Xiao-Juan Zhong Han-Du Guo Si-Han Jin Xi Li Ling-Xia Sun https://doi.org/10.1590/1678-4685-GMB-2015-0228 https://doaj.org/article/c4eb5f4b7dda40f999e8cb9b615e9a39 EN eng Sociedade Brasileira de Genética http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016005008106&lng=en&tlng=en https://doaj.org/toc/1678-4685 1678-4685 doi:10.1590/1678-4685-GMB-2015-0228 https://doaj.org/article/c4eb5f4b7dda40f999e8cb9b615e9a39 Genetics and Molecular Biology, Iss 0 DREB1 expression profile freezing stress Iceland poppy transcription factor Genetics QH426-470 article ftdoajarticles https://doi.org/10.1590/1678-4685-GMB-2015-0228 2022-12-31T08:21:21Z Abstract DREB1 of the AP2/ERF superfamily plays a key role in the regulation of plant response to low temperatures. In this study, a novel DREB1/CBF transcription factor, PnDREB1, was isolated from Iceland poppy (Papaver nudicaule), a plant adaptive to low temperature environments. It is homologous to the known DREB1s of Arabidopsis and other plant species. It also shares similar 3D structure, and conserved and functionally important motifs with DREB1s of Arabidopsis. The phylogenetic analysis indicated that the AP2 domain of PnDREB1 is similar to those of Glycine max, Medicago truncatula, and M. sativa. PnDREB1 is constitutively expressed in diverse tissues and is increased in roots. qPCR analyses indicated that PnDREB1 is significantly induced by freezing treatment as well as by abscissic acid. The expression levels induced by freezing treatment were higher in the variety with higher degree of freezing tolerance. These results suggested that PnDREB1 is a novel and functional DREB1 transcription factor involved in freezing response and possibly in other abiotic stresses. Furthermore, the freezing-induction could be suppressed by exogenous gibberellins acid, indicating that PnDREB1 might play some role in the GA signaling transduction pathway. This study provides a basis for better understanding the roles of DREB1 in adaption of Iceland poppy to low temperatures. Article in Journal/Newspaper Iceland Directory of Open Access Journals: DOAJ Articles Genetics and Molecular Biology 39 4 616 628 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
DREB1 expression profile freezing stress Iceland poppy transcription factor Genetics QH426-470 |
spellingShingle |
DREB1 expression profile freezing stress Iceland poppy transcription factor Genetics QH426-470 Zhuo Huang Jiao He Xiao-Juan Zhong Han-Du Guo Si-Han Jin Xi Li Ling-Xia Sun Molecular cloning and characterization of a novel freezing-inducible DREB1/CBF transcription factor gene in boreal plant Iceland poppy (Papaver nudicaule) |
topic_facet |
DREB1 expression profile freezing stress Iceland poppy transcription factor Genetics QH426-470 |
description |
Abstract DREB1 of the AP2/ERF superfamily plays a key role in the regulation of plant response to low temperatures. In this study, a novel DREB1/CBF transcription factor, PnDREB1, was isolated from Iceland poppy (Papaver nudicaule), a plant adaptive to low temperature environments. It is homologous to the known DREB1s of Arabidopsis and other plant species. It also shares similar 3D structure, and conserved and functionally important motifs with DREB1s of Arabidopsis. The phylogenetic analysis indicated that the AP2 domain of PnDREB1 is similar to those of Glycine max, Medicago truncatula, and M. sativa. PnDREB1 is constitutively expressed in diverse tissues and is increased in roots. qPCR analyses indicated that PnDREB1 is significantly induced by freezing treatment as well as by abscissic acid. The expression levels induced by freezing treatment were higher in the variety with higher degree of freezing tolerance. These results suggested that PnDREB1 is a novel and functional DREB1 transcription factor involved in freezing response and possibly in other abiotic stresses. Furthermore, the freezing-induction could be suppressed by exogenous gibberellins acid, indicating that PnDREB1 might play some role in the GA signaling transduction pathway. This study provides a basis for better understanding the roles of DREB1 in adaption of Iceland poppy to low temperatures. |
format |
Article in Journal/Newspaper |
author |
Zhuo Huang Jiao He Xiao-Juan Zhong Han-Du Guo Si-Han Jin Xi Li Ling-Xia Sun |
author_facet |
Zhuo Huang Jiao He Xiao-Juan Zhong Han-Du Guo Si-Han Jin Xi Li Ling-Xia Sun |
author_sort |
Zhuo Huang |
title |
Molecular cloning and characterization of a novel freezing-inducible DREB1/CBF transcription factor gene in boreal plant Iceland poppy (Papaver nudicaule) |
title_short |
Molecular cloning and characterization of a novel freezing-inducible DREB1/CBF transcription factor gene in boreal plant Iceland poppy (Papaver nudicaule) |
title_full |
Molecular cloning and characterization of a novel freezing-inducible DREB1/CBF transcription factor gene in boreal plant Iceland poppy (Papaver nudicaule) |
title_fullStr |
Molecular cloning and characterization of a novel freezing-inducible DREB1/CBF transcription factor gene in boreal plant Iceland poppy (Papaver nudicaule) |
title_full_unstemmed |
Molecular cloning and characterization of a novel freezing-inducible DREB1/CBF transcription factor gene in boreal plant Iceland poppy (Papaver nudicaule) |
title_sort |
molecular cloning and characterization of a novel freezing-inducible dreb1/cbf transcription factor gene in boreal plant iceland poppy (papaver nudicaule) |
publisher |
Sociedade Brasileira de Genética |
url |
https://doi.org/10.1590/1678-4685-GMB-2015-0228 https://doaj.org/article/c4eb5f4b7dda40f999e8cb9b615e9a39 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Genetics and Molecular Biology, Iss 0 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016005008106&lng=en&tlng=en https://doaj.org/toc/1678-4685 1678-4685 doi:10.1590/1678-4685-GMB-2015-0228 https://doaj.org/article/c4eb5f4b7dda40f999e8cb9b615e9a39 |
op_doi |
https://doi.org/10.1590/1678-4685-GMB-2015-0228 |
container_title |
Genetics and Molecular Biology |
container_volume |
39 |
container_issue |
4 |
container_start_page |
616 |
op_container_end_page |
628 |
_version_ |
1766036358955008000 |