Physiological Responses to Oxidative Stress of Different Chlorella vulgaris Isolates
Oxidizing agents, notably hydrogen peroxide (H2O2), are increasingly used in a number of applications. Their manner of action on algae was investigated in this work. Different concentrations of H2O2 and silver ions were applied to the algal cultures to follow the effect of their action in antarctic...
Published in: | International Journal Bioautomation |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Bulgarian Academy of Sciences
2019
|
Subjects: | |
Online Access: | https://doi.org/10.7546/ijba.2019.23.4.000594 https://doaj.org/article/c4eaef87fb2e474fbe7aafa73e4baeb8 |
id |
ftdoajarticles:oai:doaj.org/article:c4eaef87fb2e474fbe7aafa73e4baeb8 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:c4eaef87fb2e474fbe7aafa73e4baeb8 2023-05-15T14:03:34+02:00 Physiological Responses to Oxidative Stress of Different Chlorella vulgaris Isolates Dilyana Doneva Juliana Ivanova Lyudmila Kabaivanova 2019-12-01T00:00:00Z https://doi.org/10.7546/ijba.2019.23.4.000594 https://doaj.org/article/c4eaef87fb2e474fbe7aafa73e4baeb8 EN eng Bulgarian Academy of Sciences http://www.biomed.bas.bg/bioautomation/2019/vol_23.4/files/23.4_05.pdf https://doaj.org/toc/1314-1902 https://doaj.org/toc/1314-2321 1314-1902 1314-2321 doi:10.7546/ijba.2019.23.4.000594 https://doaj.org/article/c4eaef87fb2e474fbe7aafa73e4baeb8 International Journal Bioautomation, Vol 23, Iss 4, Pp 447-460 (2019) chlorella vulgaris antarctic oxidative stress photosynthesis enzyme profile Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.7546/ijba.2019.23.4.000594 2022-12-31T00:51:35Z Oxidizing agents, notably hydrogen peroxide (H2O2), are increasingly used in a number of applications. Their manner of action on algae was investigated in this work. Different concentrations of H2O2 and silver ions were applied to the algal cultures to follow the effect of their action in antarctic and mesophilic isolates of the green alga Chlorella vulgaris. Oxidative stress-induced physiological responses manifested by changes in the photosynthetic apparatus and antioxidant enzymes were estimated. The algae from the antarctic region appeared to be less resistant to oxidative stress than their mesophilic counterparts, concerning growth after treatment with higher concentrations of the reagent. The increased concentration (0.05%) lead to raise of activity and isoenzyme number of catalase (CAT) in both C. vulgaris strains. The isoenzyme activity of peroxidase (POX) decreased and disappeared in the antarctic isolate. At the same time the highest concentration (0.08%) provoked restoration of POX activity in the mesophylic one. Decrease in superoxide dismutase (SOD) activity was registered and this effect was more intensive in the moderate and fast mobile isoenzymes. Isoenzyme profile at 0.08% reagent concentration for the mesophylic isolate was similar with this at 0.05% for the antarctic isolate and a fourth isoenzyme appeared. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic International Journal Bioautomation 23 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
chlorella vulgaris antarctic oxidative stress photosynthesis enzyme profile Biology (General) QH301-705.5 |
spellingShingle |
chlorella vulgaris antarctic oxidative stress photosynthesis enzyme profile Biology (General) QH301-705.5 Dilyana Doneva Juliana Ivanova Lyudmila Kabaivanova Physiological Responses to Oxidative Stress of Different Chlorella vulgaris Isolates |
topic_facet |
chlorella vulgaris antarctic oxidative stress photosynthesis enzyme profile Biology (General) QH301-705.5 |
description |
Oxidizing agents, notably hydrogen peroxide (H2O2), are increasingly used in a number of applications. Their manner of action on algae was investigated in this work. Different concentrations of H2O2 and silver ions were applied to the algal cultures to follow the effect of their action in antarctic and mesophilic isolates of the green alga Chlorella vulgaris. Oxidative stress-induced physiological responses manifested by changes in the photosynthetic apparatus and antioxidant enzymes were estimated. The algae from the antarctic region appeared to be less resistant to oxidative stress than their mesophilic counterparts, concerning growth after treatment with higher concentrations of the reagent. The increased concentration (0.05%) lead to raise of activity and isoenzyme number of catalase (CAT) in both C. vulgaris strains. The isoenzyme activity of peroxidase (POX) decreased and disappeared in the antarctic isolate. At the same time the highest concentration (0.08%) provoked restoration of POX activity in the mesophylic one. Decrease in superoxide dismutase (SOD) activity was registered and this effect was more intensive in the moderate and fast mobile isoenzymes. Isoenzyme profile at 0.08% reagent concentration for the mesophylic isolate was similar with this at 0.05% for the antarctic isolate and a fourth isoenzyme appeared. |
format |
Article in Journal/Newspaper |
author |
Dilyana Doneva Juliana Ivanova Lyudmila Kabaivanova |
author_facet |
Dilyana Doneva Juliana Ivanova Lyudmila Kabaivanova |
author_sort |
Dilyana Doneva |
title |
Physiological Responses to Oxidative Stress of Different Chlorella vulgaris Isolates |
title_short |
Physiological Responses to Oxidative Stress of Different Chlorella vulgaris Isolates |
title_full |
Physiological Responses to Oxidative Stress of Different Chlorella vulgaris Isolates |
title_fullStr |
Physiological Responses to Oxidative Stress of Different Chlorella vulgaris Isolates |
title_full_unstemmed |
Physiological Responses to Oxidative Stress of Different Chlorella vulgaris Isolates |
title_sort |
physiological responses to oxidative stress of different chlorella vulgaris isolates |
publisher |
Bulgarian Academy of Sciences |
publishDate |
2019 |
url |
https://doi.org/10.7546/ijba.2019.23.4.000594 https://doaj.org/article/c4eaef87fb2e474fbe7aafa73e4baeb8 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
International Journal Bioautomation, Vol 23, Iss 4, Pp 447-460 (2019) |
op_relation |
http://www.biomed.bas.bg/bioautomation/2019/vol_23.4/files/23.4_05.pdf https://doaj.org/toc/1314-1902 https://doaj.org/toc/1314-2321 1314-1902 1314-2321 doi:10.7546/ijba.2019.23.4.000594 https://doaj.org/article/c4eaef87fb2e474fbe7aafa73e4baeb8 |
op_doi |
https://doi.org/10.7546/ijba.2019.23.4.000594 |
container_title |
International Journal Bioautomation |
container_volume |
23 |
container_issue |
4 |
_version_ |
1766274269949460480 |