Zika virus infection in Nicaraguan households.
Zika virus (ZIKV) infection recently caused major epidemics in the Americas and is linked to congenital birth defects and Guillain-Barré Syndrome. A pilot study of ZIKV infection in Nicaraguan households was conducted from August 31 to October 21, 2016, in Managua, Nicaragua. We enrolled 33 laborato...
Published in: | PLOS Neglected Tropical Diseases |
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Online Access: | https://doi.org/10.1371/journal.pntd.0006518 https://doaj.org/article/c4e6e97fd9b84b31b5bf9732e3303c9c |
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ftdoajarticles:oai:doaj.org/article:c4e6e97fd9b84b31b5bf9732e3303c9c 2023-05-15T15:15:01+02:00 Zika virus infection in Nicaraguan households. Raquel Burger-Calderon Karla Gonzalez Sergio Ojeda José Victor Zambrana Nery Sanchez Cristhiam Cerpas Cruz Harold Suazo Laguna Fausto Bustos Miguel Plazaola Brenda Lopez Mercado Douglas Elizondo Sonia Arguello Jairo Carey Monterrey Andrea Nuñez Josefina Coloma Jesse J Waggoner Aubree Gordon Guillermina Kuan Angel Balmaseda Eva Harris 2018-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006518 https://doaj.org/article/c4e6e97fd9b84b31b5bf9732e3303c9c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6014677?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006518 https://doaj.org/article/c4e6e97fd9b84b31b5bf9732e3303c9c PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006518 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006518 2022-12-31T13:21:22Z Zika virus (ZIKV) infection recently caused major epidemics in the Americas and is linked to congenital birth defects and Guillain-Barré Syndrome. A pilot study of ZIKV infection in Nicaraguan households was conducted from August 31 to October 21, 2016, in Managua, Nicaragua. We enrolled 33 laboratory-confirmed Zika index cases and their household members (109 contacts) and followed them on days 3-4, 6-7, 9-10, and 21, collecting serum/plasma, urine, and saliva specimens along with clinical, demographic, and socio-economic status information. Collected samples were processed by rRT-PCR to determine viral load (VL) and duration of detectable ZIKV RNA in human bodily fluids. At enrollment, 11 (10%) contacts were ZIKV rRT-PCR-positive and 23 (21%) were positive by IgM antibodies; 3 incident cases were detected during the study period. Twenty of 33 (61%) index households had contacts with ZIKV infection, with an average of 1.9 (range 1-6) positive contacts per household, and in 60% of these households, ≥50% of the members were positive for ZIKV infection. Analysis of clinical information allowed us to estimate the symptomatic to asymptomatic (S:A) ratio of 14:23 (1:1.6) among the contacts, finding 62% of the infections to be asymptomatic. The maximum number of days during which ZIKV RNA was detected was 7 days post-symptom onset in saliva and serum/plasma and 22 days in urine. Overall, VL levels in serum/plasma, saliva, and urine specimens were comparable, with means of 5.6, 5.3 and 4.5 log10 copies/ml respectively, with serum attaining the highest VL peak at 8.1 log10 copies/ml. Detecting ZIKV RNA in saliva over a similar time-period and level as in serum/plasma indicates that saliva could potentially serve as a more accessible diagnostic sample. Finding the majority of infections to be asymptomatic emphasizes the importance of silent ZIKV transmission and helps inform public health interventions in the region and globally. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Barré ENVELOPE(-68.550,-68.550,-67.500,-67.500) PLOS Neglected Tropical Diseases 12 5 e0006518 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Raquel Burger-Calderon Karla Gonzalez Sergio Ojeda José Victor Zambrana Nery Sanchez Cristhiam Cerpas Cruz Harold Suazo Laguna Fausto Bustos Miguel Plazaola Brenda Lopez Mercado Douglas Elizondo Sonia Arguello Jairo Carey Monterrey Andrea Nuñez Josefina Coloma Jesse J Waggoner Aubree Gordon Guillermina Kuan Angel Balmaseda Eva Harris Zika virus infection in Nicaraguan households. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Zika virus (ZIKV) infection recently caused major epidemics in the Americas and is linked to congenital birth defects and Guillain-Barré Syndrome. A pilot study of ZIKV infection in Nicaraguan households was conducted from August 31 to October 21, 2016, in Managua, Nicaragua. We enrolled 33 laboratory-confirmed Zika index cases and their household members (109 contacts) and followed them on days 3-4, 6-7, 9-10, and 21, collecting serum/plasma, urine, and saliva specimens along with clinical, demographic, and socio-economic status information. Collected samples were processed by rRT-PCR to determine viral load (VL) and duration of detectable ZIKV RNA in human bodily fluids. At enrollment, 11 (10%) contacts were ZIKV rRT-PCR-positive and 23 (21%) were positive by IgM antibodies; 3 incident cases were detected during the study period. Twenty of 33 (61%) index households had contacts with ZIKV infection, with an average of 1.9 (range 1-6) positive contacts per household, and in 60% of these households, ≥50% of the members were positive for ZIKV infection. Analysis of clinical information allowed us to estimate the symptomatic to asymptomatic (S:A) ratio of 14:23 (1:1.6) among the contacts, finding 62% of the infections to be asymptomatic. The maximum number of days during which ZIKV RNA was detected was 7 days post-symptom onset in saliva and serum/plasma and 22 days in urine. Overall, VL levels in serum/plasma, saliva, and urine specimens were comparable, with means of 5.6, 5.3 and 4.5 log10 copies/ml respectively, with serum attaining the highest VL peak at 8.1 log10 copies/ml. Detecting ZIKV RNA in saliva over a similar time-period and level as in serum/plasma indicates that saliva could potentially serve as a more accessible diagnostic sample. Finding the majority of infections to be asymptomatic emphasizes the importance of silent ZIKV transmission and helps inform public health interventions in the region and globally. |
format |
Article in Journal/Newspaper |
author |
Raquel Burger-Calderon Karla Gonzalez Sergio Ojeda José Victor Zambrana Nery Sanchez Cristhiam Cerpas Cruz Harold Suazo Laguna Fausto Bustos Miguel Plazaola Brenda Lopez Mercado Douglas Elizondo Sonia Arguello Jairo Carey Monterrey Andrea Nuñez Josefina Coloma Jesse J Waggoner Aubree Gordon Guillermina Kuan Angel Balmaseda Eva Harris |
author_facet |
Raquel Burger-Calderon Karla Gonzalez Sergio Ojeda José Victor Zambrana Nery Sanchez Cristhiam Cerpas Cruz Harold Suazo Laguna Fausto Bustos Miguel Plazaola Brenda Lopez Mercado Douglas Elizondo Sonia Arguello Jairo Carey Monterrey Andrea Nuñez Josefina Coloma Jesse J Waggoner Aubree Gordon Guillermina Kuan Angel Balmaseda Eva Harris |
author_sort |
Raquel Burger-Calderon |
title |
Zika virus infection in Nicaraguan households. |
title_short |
Zika virus infection in Nicaraguan households. |
title_full |
Zika virus infection in Nicaraguan households. |
title_fullStr |
Zika virus infection in Nicaraguan households. |
title_full_unstemmed |
Zika virus infection in Nicaraguan households. |
title_sort |
zika virus infection in nicaraguan households. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006518 https://doaj.org/article/c4e6e97fd9b84b31b5bf9732e3303c9c |
long_lat |
ENVELOPE(-68.550,-68.550,-67.500,-67.500) |
geographic |
Arctic Barré |
geographic_facet |
Arctic Barré |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006518 (2018) |
op_relation |
http://europepmc.org/articles/PMC6014677?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006518 https://doaj.org/article/c4e6e97fd9b84b31b5bf9732e3303c9c |
op_doi |
https://doi.org/10.1371/journal.pntd.0006518 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
5 |
container_start_page |
e0006518 |
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1766345399421894656 |