Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation
Exploring the molecular mechanisms behind bacterial adaptation to extreme temperatures has potential biotechnological applications. In the present study, Pseudomonas sp. Lz4W, a Gram-negative psychrophilic bacterium adapted to survive in Antarctica, was selected to decipher the molecular mechanism u...
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ftdoajarticles:oai:doaj.org/article:c4e2cf6ff8634c759a18d375782aaa97 2023-05-15T13:31:16+02:00 Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation Johny Ijaq Deepika Chandra Malay Kumar Ray M. V. Jagannadham 2022-03-01T00:00:00Z https://doi.org/10.3389/fgene.2022.825269 https://doaj.org/article/c4e2cf6ff8634c759a18d375782aaa97 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fgene.2022.825269/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2022.825269 https://doaj.org/article/c4e2cf6ff8634c759a18d375782aaa97 Frontiers in Genetics, Vol 13 (2022) Pseudomonas sp. Lz4W LC-MS/MS hypothetical proteins cold adaptation functional annotation Genetics QH426-470 article 2022 ftdoajarticles https://doi.org/10.3389/fgene.2022.825269 2022-12-31T15:51:53Z Exploring the molecular mechanisms behind bacterial adaptation to extreme temperatures has potential biotechnological applications. In the present study, Pseudomonas sp. Lz4W, a Gram-negative psychrophilic bacterium adapted to survive in Antarctica, was selected to decipher the molecular mechanism underlying the cold adaptation. Proteome analysis of the isolates grown at 4°C was performed to identify the proteins and pathways that are responsible for the adaptation. However, many proteins from the expressed proteome were found to be hypothetical proteins (HPs), whose function is unknown. Investigating the functional roles of these proteins may provide additional information in the biological understanding of the bacterial cold adaptation. Thus, our study aimed to assign functions to these HPs and understand their role at the molecular level. We used a structured insilico workflow combining different bioinformatics tools and databases for functional annotation. Pseudomonas sp. Lz4W genome (CP017432, version 1) contains 4493 genes and 4412 coding sequences (CDS), of which 743 CDS were annotated as HPs. Of these, from the proteome analysis, 61 HPs were found to be expressed consistently at the protein level. The amino acid sequences of these 61 HPs were submitted to our workflow and we could successfully assign a function to 18 HPs. Most of these proteins were predicted to be involved in biological mechanisms of cold adaptations such as peptidoglycan metabolism, cell wall organization, ATP hydrolysis, outer membrane fluidity, catalysis, and others. This study provided a better understanding of the functional significance of HPs in cold adaptation of Pseudomonas sp. Lz4W. Our approach emphasizes the importance of addressing the “hypothetical protein problem” for a thorough understanding of mechanisms at the cellular level, as well as, provided the assessment of integrating proteomics methods with various annotation and curation approaches to characterize hypothetical or uncharacterized protein data. The MS ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Frontiers in Genetics 13 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pseudomonas sp. Lz4W LC-MS/MS hypothetical proteins cold adaptation functional annotation Genetics QH426-470 |
spellingShingle |
Pseudomonas sp. Lz4W LC-MS/MS hypothetical proteins cold adaptation functional annotation Genetics QH426-470 Johny Ijaq Deepika Chandra Malay Kumar Ray M. V. Jagannadham Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation |
topic_facet |
Pseudomonas sp. Lz4W LC-MS/MS hypothetical proteins cold adaptation functional annotation Genetics QH426-470 |
description |
Exploring the molecular mechanisms behind bacterial adaptation to extreme temperatures has potential biotechnological applications. In the present study, Pseudomonas sp. Lz4W, a Gram-negative psychrophilic bacterium adapted to survive in Antarctica, was selected to decipher the molecular mechanism underlying the cold adaptation. Proteome analysis of the isolates grown at 4°C was performed to identify the proteins and pathways that are responsible for the adaptation. However, many proteins from the expressed proteome were found to be hypothetical proteins (HPs), whose function is unknown. Investigating the functional roles of these proteins may provide additional information in the biological understanding of the bacterial cold adaptation. Thus, our study aimed to assign functions to these HPs and understand their role at the molecular level. We used a structured insilico workflow combining different bioinformatics tools and databases for functional annotation. Pseudomonas sp. Lz4W genome (CP017432, version 1) contains 4493 genes and 4412 coding sequences (CDS), of which 743 CDS were annotated as HPs. Of these, from the proteome analysis, 61 HPs were found to be expressed consistently at the protein level. The amino acid sequences of these 61 HPs were submitted to our workflow and we could successfully assign a function to 18 HPs. Most of these proteins were predicted to be involved in biological mechanisms of cold adaptations such as peptidoglycan metabolism, cell wall organization, ATP hydrolysis, outer membrane fluidity, catalysis, and others. This study provided a better understanding of the functional significance of HPs in cold adaptation of Pseudomonas sp. Lz4W. Our approach emphasizes the importance of addressing the “hypothetical protein problem” for a thorough understanding of mechanisms at the cellular level, as well as, provided the assessment of integrating proteomics methods with various annotation and curation approaches to characterize hypothetical or uncharacterized protein data. The MS ... |
format |
Article in Journal/Newspaper |
author |
Johny Ijaq Deepika Chandra Malay Kumar Ray M. V. Jagannadham |
author_facet |
Johny Ijaq Deepika Chandra Malay Kumar Ray M. V. Jagannadham |
author_sort |
Johny Ijaq |
title |
Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation |
title_short |
Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation |
title_full |
Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation |
title_fullStr |
Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation |
title_full_unstemmed |
Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation |
title_sort |
investigating the functional role of hypothetical proteins from an antarctic bacterium pseudomonas sp. lz4w: emphasis on identifying proteins involved in cold adaptation |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fgene.2022.825269 https://doaj.org/article/c4e2cf6ff8634c759a18d375782aaa97 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Frontiers in Genetics, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fgene.2022.825269/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2022.825269 https://doaj.org/article/c4e2cf6ff8634c759a18d375782aaa97 |
op_doi |
https://doi.org/10.3389/fgene.2022.825269 |
container_title |
Frontiers in Genetics |
container_volume |
13 |
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1766017129006497792 |