Sequence analysis of the genome of piscine orthoreovirus (PRV) associated with heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar).
Piscine orthoreovirus (PRV) is associated with heart- and skeletal muscle inflammation (HSMI) of farmed Atlantic salmon (Salmo salar). We have performed detailed sequence analysis of the PRV genome with focus on putative encoded proteins, compared with prototype strains from mammalian (MRV T3D)- and...
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ftdoajarticles:oai:doaj.org/article:c4ded1966a284a299a5d7abcc5193a91 2023-05-15T15:32:00+02:00 Sequence analysis of the genome of piscine orthoreovirus (PRV) associated with heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar). Turhan Markussen Maria K Dahle Torstein Tengs Marie Løvoll Øystein W Finstad Christer R Wiik-Nielsen Søren Grove Silje Lauksund Børre Robertsen Espen Rimstad 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0070075 https://doaj.org/article/c4ded1966a284a299a5d7abcc5193a91 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3726481?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0070075 https://doaj.org/article/c4ded1966a284a299a5d7abcc5193a91 PLoS ONE, Vol 8, Iss 7, p e70075 (2013) Medicine R Science Q article 2013 ftdoajarticles https://doi.org/10.1371/journal.pone.0070075 2022-12-31T09:16:41Z Piscine orthoreovirus (PRV) is associated with heart- and skeletal muscle inflammation (HSMI) of farmed Atlantic salmon (Salmo salar). We have performed detailed sequence analysis of the PRV genome with focus on putative encoded proteins, compared with prototype strains from mammalian (MRV T3D)- and avian orthoreoviruses (ARV-138), and aquareovirus (GCRV-873). Amino acid identities were low for most gene segments but detailed sequence analysis showed that many protein motifs or key amino acid residues known to be central to protein function are conserved for most PRV proteins. For M-class proteins this included a proline residue in μ2 which, for MRV, has been shown to play a key role in both the formation and structural organization of virus inclusion bodies, and affect interferon-β signaling and induction of myocarditis. Predicted structural similarities in the inner core-forming proteins λ1 and σ2 suggest a conserved core structure. In contrast, low amino acid identities in the predicted PRV surface proteins μ1, σ1 and σ3 suggested differences regarding cellular interactions between the reovirus genera. However, for σ1, amino acid residues central for MRV binding to sialic acids, and cleavage- and myristoylation sites in μ1 required for endosomal membrane penetration during infection are partially or wholly conserved in the homologous PRV proteins. In PRV σ3 the only conserved element found was a zinc finger motif. We provide evidence that the S1 segment encoding σ3 also encodes a 124 aa (p13) protein, which appears to be localized to intracellular Golgi-like structures. The S2 and L2 gene segments are also potentially polycistronic, predicted to encode a 71 aa- (p8) and a 98 aa (p11) protein, respectively. It is concluded that PRV has more properties in common with orthoreoviruses than with aquareoviruses. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles PLoS ONE 8 7 e70075 |
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language |
English |
topic |
Medicine R Science Q |
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Medicine R Science Q Turhan Markussen Maria K Dahle Torstein Tengs Marie Løvoll Øystein W Finstad Christer R Wiik-Nielsen Søren Grove Silje Lauksund Børre Robertsen Espen Rimstad Sequence analysis of the genome of piscine orthoreovirus (PRV) associated with heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar). |
topic_facet |
Medicine R Science Q |
description |
Piscine orthoreovirus (PRV) is associated with heart- and skeletal muscle inflammation (HSMI) of farmed Atlantic salmon (Salmo salar). We have performed detailed sequence analysis of the PRV genome with focus on putative encoded proteins, compared with prototype strains from mammalian (MRV T3D)- and avian orthoreoviruses (ARV-138), and aquareovirus (GCRV-873). Amino acid identities were low for most gene segments but detailed sequence analysis showed that many protein motifs or key amino acid residues known to be central to protein function are conserved for most PRV proteins. For M-class proteins this included a proline residue in μ2 which, for MRV, has been shown to play a key role in both the formation and structural organization of virus inclusion bodies, and affect interferon-β signaling and induction of myocarditis. Predicted structural similarities in the inner core-forming proteins λ1 and σ2 suggest a conserved core structure. In contrast, low amino acid identities in the predicted PRV surface proteins μ1, σ1 and σ3 suggested differences regarding cellular interactions between the reovirus genera. However, for σ1, amino acid residues central for MRV binding to sialic acids, and cleavage- and myristoylation sites in μ1 required for endosomal membrane penetration during infection are partially or wholly conserved in the homologous PRV proteins. In PRV σ3 the only conserved element found was a zinc finger motif. We provide evidence that the S1 segment encoding σ3 also encodes a 124 aa (p13) protein, which appears to be localized to intracellular Golgi-like structures. The S2 and L2 gene segments are also potentially polycistronic, predicted to encode a 71 aa- (p8) and a 98 aa (p11) protein, respectively. It is concluded that PRV has more properties in common with orthoreoviruses than with aquareoviruses. |
format |
Article in Journal/Newspaper |
author |
Turhan Markussen Maria K Dahle Torstein Tengs Marie Løvoll Øystein W Finstad Christer R Wiik-Nielsen Søren Grove Silje Lauksund Børre Robertsen Espen Rimstad |
author_facet |
Turhan Markussen Maria K Dahle Torstein Tengs Marie Løvoll Øystein W Finstad Christer R Wiik-Nielsen Søren Grove Silje Lauksund Børre Robertsen Espen Rimstad |
author_sort |
Turhan Markussen |
title |
Sequence analysis of the genome of piscine orthoreovirus (PRV) associated with heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar). |
title_short |
Sequence analysis of the genome of piscine orthoreovirus (PRV) associated with heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar). |
title_full |
Sequence analysis of the genome of piscine orthoreovirus (PRV) associated with heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar). |
title_fullStr |
Sequence analysis of the genome of piscine orthoreovirus (PRV) associated with heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar). |
title_full_unstemmed |
Sequence analysis of the genome of piscine orthoreovirus (PRV) associated with heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar). |
title_sort |
sequence analysis of the genome of piscine orthoreovirus (prv) associated with heart and skeletal muscle inflammation (hsmi) in atlantic salmon (salmo salar). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pone.0070075 https://doaj.org/article/c4ded1966a284a299a5d7abcc5193a91 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
PLoS ONE, Vol 8, Iss 7, p e70075 (2013) |
op_relation |
http://europepmc.org/articles/PMC3726481?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0070075 https://doaj.org/article/c4ded1966a284a299a5d7abcc5193a91 |
op_doi |
https://doi.org/10.1371/journal.pone.0070075 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
7 |
container_start_page |
e70075 |
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