Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains
Climate warming is accelerating permafrost degradation. Soil microorganisms play key roles in the maintenance of high-latitude permafrost regions and forest ecosystems’ functioning and regulation of biogeochemical cycles. In this study, we used Illumina MiSeq high-throughput sequencing to investigat...
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MDPI AG
2022
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ftdoajarticles:oai:doaj.org/article:c4d58e608a3645bebacc87c7bfb278d9 2023-05-15T17:57:13+02:00 Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains Dandan Song Yuanquan Cui Dalong Ma Xin Li Lin Liu 2022-07-01T00:00:00Z https://doi.org/10.3390/su14159284 https://doaj.org/article/c4d58e608a3645bebacc87c7bfb278d9 EN eng MDPI AG https://www.mdpi.com/2071-1050/14/15/9284 https://doaj.org/toc/2071-1050 doi:10.3390/su14159284 2071-1050 https://doaj.org/article/c4d58e608a3645bebacc87c7bfb278d9 Sustainability, Vol 14, Iss 9284, p 9284 (2022) microbial community cold regions coniferous forest ecological filters environmental harshness Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 article 2022 ftdoajarticles https://doi.org/10.3390/su14159284 2022-12-30T20:39:38Z Climate warming is accelerating permafrost degradation. Soil microorganisms play key roles in the maintenance of high-latitude permafrost regions and forest ecosystems’ functioning and regulation of biogeochemical cycles. In this study, we used Illumina MiSeq high-throughput sequencing to investigate soil bacterial community composition at a primeval Larix gmelinii forest and a secondary Betula platyphylla forest in a permafrost region of the Greater Xing’an Mountains. The Shannon diversity index tended to decrease and then increase with increasing soil depth, which was significantly higher in the L. gmelinii forest than in the B. platyphylla forest at 40–60 cm. Proteobacteria (19.86–29.68%), Acidobacteria (13.59–31.44%), Chloroflexi (11.04–27.19%), Actinobacteria (7.05–25.57%), Gemmatimonadetes (1.76–9.18%), and Verrucomicrobia (2.03–7.00%) were the predominant phyla of the bacterial community in two forest types. The relative abundance of Proteobacteria showed a decreasing trend in the B. platyphylla forest and an increasing trend in the L. gmelinii forest, whereas that of Chloroflexi increased and then decreased in the B. platyphylla forest and decreased in the L. gmelinii forest with increasing soil depth. The relative abundance of Acidobacteria was significantly higher in the B. platyphylla forest than in the L. gmelinii forest at 0–20 cm depth, whereas that of Actinobacteria was significantly higher in the L. gmelinii forest than in the B. platyphylla forest at 0–20 cm and 40–60 cm depth. Principal coordinate analysis (PCoA) and two-way analysis of variance (ANOVA) indicated that microbial community composition was more significantly influenced by forest type than soil depth. Redundancy analysis (RDA) showed that microbial community structure was strongly affected by soil physicochemical properties such as nitrate nitrogen (NO3−-N), pH, and total organic carbon (TOC). These results offer insights into the potential relationship between soil microbial community and forest conversion in high latitude ... Article in Journal/Newspaper permafrost Directory of Open Access Journals: DOAJ Articles Sustainability 14 15 9284 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
microbial community cold regions coniferous forest ecological filters environmental harshness Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 |
spellingShingle |
microbial community cold regions coniferous forest ecological filters environmental harshness Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 Dandan Song Yuanquan Cui Dalong Ma Xin Li Lin Liu Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains |
topic_facet |
microbial community cold regions coniferous forest ecological filters environmental harshness Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 |
description |
Climate warming is accelerating permafrost degradation. Soil microorganisms play key roles in the maintenance of high-latitude permafrost regions and forest ecosystems’ functioning and regulation of biogeochemical cycles. In this study, we used Illumina MiSeq high-throughput sequencing to investigate soil bacterial community composition at a primeval Larix gmelinii forest and a secondary Betula platyphylla forest in a permafrost region of the Greater Xing’an Mountains. The Shannon diversity index tended to decrease and then increase with increasing soil depth, which was significantly higher in the L. gmelinii forest than in the B. platyphylla forest at 40–60 cm. Proteobacteria (19.86–29.68%), Acidobacteria (13.59–31.44%), Chloroflexi (11.04–27.19%), Actinobacteria (7.05–25.57%), Gemmatimonadetes (1.76–9.18%), and Verrucomicrobia (2.03–7.00%) were the predominant phyla of the bacterial community in two forest types. The relative abundance of Proteobacteria showed a decreasing trend in the B. platyphylla forest and an increasing trend in the L. gmelinii forest, whereas that of Chloroflexi increased and then decreased in the B. platyphylla forest and decreased in the L. gmelinii forest with increasing soil depth. The relative abundance of Acidobacteria was significantly higher in the B. platyphylla forest than in the L. gmelinii forest at 0–20 cm depth, whereas that of Actinobacteria was significantly higher in the L. gmelinii forest than in the B. platyphylla forest at 0–20 cm and 40–60 cm depth. Principal coordinate analysis (PCoA) and two-way analysis of variance (ANOVA) indicated that microbial community composition was more significantly influenced by forest type than soil depth. Redundancy analysis (RDA) showed that microbial community structure was strongly affected by soil physicochemical properties such as nitrate nitrogen (NO3−-N), pH, and total organic carbon (TOC). These results offer insights into the potential relationship between soil microbial community and forest conversion in high latitude ... |
format |
Article in Journal/Newspaper |
author |
Dandan Song Yuanquan Cui Dalong Ma Xin Li Lin Liu |
author_facet |
Dandan Song Yuanquan Cui Dalong Ma Xin Li Lin Liu |
author_sort |
Dandan Song |
title |
Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains |
title_short |
Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains |
title_full |
Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains |
title_fullStr |
Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains |
title_full_unstemmed |
Spatial Variation of Microbial Community Structure and Its Driving Environmental Factors in Two Forest Types in Permafrost Region of Greater Xing′an Mountains |
title_sort |
spatial variation of microbial community structure and its driving environmental factors in two forest types in permafrost region of greater xing′an mountains |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/su14159284 https://doaj.org/article/c4d58e608a3645bebacc87c7bfb278d9 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
Sustainability, Vol 14, Iss 9284, p 9284 (2022) |
op_relation |
https://www.mdpi.com/2071-1050/14/15/9284 https://doaj.org/toc/2071-1050 doi:10.3390/su14159284 2071-1050 https://doaj.org/article/c4d58e608a3645bebacc87c7bfb278d9 |
op_doi |
https://doi.org/10.3390/su14159284 |
container_title |
Sustainability |
container_volume |
14 |
container_issue |
15 |
container_start_page |
9284 |
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1766165613016776704 |