Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure
Abstract Background Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short- and medium-term PM10 exposure. Methods Whole genome gene expression was measured in peripheral blood of 9...
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ftdoajarticles:oai:doaj.org/article:c46f8dcc87da44978a228c684074af7e 2023-05-15T15:52:55+02:00 Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure Ellen Winckelmans Tim S Nawrot Maria Tsamou Elly Den Hond Willy Baeyens Jos Kleinjans Wouter Lefebvre Nicolas Van Larebeke Martien Peusens Michelle Plusquin Hans Reynders Greet Schoeters Charlotte Vanpoucke Theo M de Kok Karen Vrijens 2017-08-01T00:00:00Z https://doi.org/10.1186/s12940-017-0292-7 https://doaj.org/article/c46f8dcc87da44978a228c684074af7e EN eng BMC http://link.springer.com/article/10.1186/s12940-017-0292-7 https://doaj.org/toc/1476-069X doi:10.1186/s12940-017-0292-7 1476-069X https://doaj.org/article/c46f8dcc87da44978a228c684074af7e Environmental Health, Vol 16, Iss 1, Pp 1-15 (2017) Ambient air pollution Particulate matter Transcriptome-wide analyses Sex-specific mitochondria Industrial medicine. Industrial hygiene RC963-969 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1186/s12940-017-0292-7 2022-12-30T22:53:03Z Abstract Background Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short- and medium-term PM10 exposure. Methods Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. Article in Journal/Newspaper Carbonic acid Directory of Open Access Journals: DOAJ Articles Environmental Health 16 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ambient air pollution Particulate matter Transcriptome-wide analyses Sex-specific mitochondria Industrial medicine. Industrial hygiene RC963-969 Public aspects of medicine RA1-1270 |
spellingShingle |
Ambient air pollution Particulate matter Transcriptome-wide analyses Sex-specific mitochondria Industrial medicine. Industrial hygiene RC963-969 Public aspects of medicine RA1-1270 Ellen Winckelmans Tim S Nawrot Maria Tsamou Elly Den Hond Willy Baeyens Jos Kleinjans Wouter Lefebvre Nicolas Van Larebeke Martien Peusens Michelle Plusquin Hans Reynders Greet Schoeters Charlotte Vanpoucke Theo M de Kok Karen Vrijens Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
topic_facet |
Ambient air pollution Particulate matter Transcriptome-wide analyses Sex-specific mitochondria Industrial medicine. Industrial hygiene RC963-969 Public aspects of medicine RA1-1270 |
description |
Abstract Background Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short- and medium-term PM10 exposure. Methods Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. |
format |
Article in Journal/Newspaper |
author |
Ellen Winckelmans Tim S Nawrot Maria Tsamou Elly Den Hond Willy Baeyens Jos Kleinjans Wouter Lefebvre Nicolas Van Larebeke Martien Peusens Michelle Plusquin Hans Reynders Greet Schoeters Charlotte Vanpoucke Theo M de Kok Karen Vrijens |
author_facet |
Ellen Winckelmans Tim S Nawrot Maria Tsamou Elly Den Hond Willy Baeyens Jos Kleinjans Wouter Lefebvre Nicolas Van Larebeke Martien Peusens Michelle Plusquin Hans Reynders Greet Schoeters Charlotte Vanpoucke Theo M de Kok Karen Vrijens |
author_sort |
Ellen Winckelmans |
title |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_short |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_fullStr |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full_unstemmed |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_sort |
transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12940-017-0292-7 https://doaj.org/article/c46f8dcc87da44978a228c684074af7e |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
Environmental Health, Vol 16, Iss 1, Pp 1-15 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12940-017-0292-7 https://doaj.org/toc/1476-069X doi:10.1186/s12940-017-0292-7 1476-069X https://doaj.org/article/c46f8dcc87da44978a228c684074af7e |
op_doi |
https://doi.org/10.1186/s12940-017-0292-7 |
container_title |
Environmental Health |
container_volume |
16 |
container_issue |
1 |
_version_ |
1766388013260079104 |