Notch Signaling Inhibition Alleviates Allergies Caused by Antarctic Krill Tropomyosin through Improving Th1/Th2 Imbalance and Modulating Gut Microbiota
Antarctic krill tropomyosin (AkTM) has been shown in mice to cause IgE-mediated food allergy. The objective of this work was to investigate the role of Notch signaling in AkTM-sensitized mice, as well as to determine the changes in gut microbiota composition and short-chain fatty acids (SCFAs) in th...
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2024
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ftdoajarticles:oai:doaj.org/article:c46166522f3e4a98bca404ce9e845f53 2024-09-15T17:48:15+00:00 Notch Signaling Inhibition Alleviates Allergies Caused by Antarctic Krill Tropomyosin through Improving Th1/Th2 Imbalance and Modulating Gut Microbiota Na Lin Hai Chi Quanyou Guo Zhidong Liu Ling Ni 2024-04-01T00:00:00Z https://doi.org/10.3390/foods13081144 https://doaj.org/article/c46166522f3e4a98bca404ce9e845f53 EN eng MDPI AG https://www.mdpi.com/2304-8158/13/8/1144 https://doaj.org/toc/2304-8158 doi:10.3390/foods13081144 2304-8158 https://doaj.org/article/c46166522f3e4a98bca404ce9e845f53 Foods, Vol 13, Iss 8, p 1144 (2024) antarctic krill tropomyosin food allergy Notch signaling Th1/Th2 imbalance gut microbiota SCFAs Chemical technology TP1-1185 article 2024 ftdoajarticles https://doi.org/10.3390/foods13081144 2024-08-05T17:49:32Z Antarctic krill tropomyosin (AkTM) has been shown in mice to cause IgE-mediated food allergy. The objective of this work was to investigate the role of Notch signaling in AkTM-sensitized mice, as well as to determine the changes in gut microbiota composition and short-chain fatty acids (SCFAs) in the allergic mice. An AkTM-induced food allergy mouse model was built and N -[ N -(3,5-difluorophenacetyl)- L -alanyl]- S -phenylglycine t-butyl ester (DAPT) was used as an γ-secretase inhibitor to inhibit the activation of Notch signaling. Food allergy indices, some key transcription factors, histologic alterations in the small intestine, and changes in gut microbiota composition were examined. The results showed that DAPT inhibited Notch signaling, which reduced AkTM-specific IgE, suppressed mast cell degranulation, decreased IL-4 but increased IFN-γ production, and alleviated allergic symptoms. Quantitative real-time PCR and Western blotting analyses revealed that expressions of Hes-1, Gata3, and IL-4 were down-regulated after DAPT treatment, accompanied by increases in T-bet and IFN-γ, indicating that Notch signaling was active in AkTM-sensitized mice and blocking it could reverse the Th1/Th2 imbalance. Expressions of key transcription factors revealed that Notch signaling could promote Th2 cell differentiation in sensitized mice. Furthermore, 16S rRNA sequencing results revealed that AkTM could alter the diversity and composition of gut microbiota in mice, leading to increases in inflammation-inducing bacteria such as Enterococcus and Escherichia-Shigella . Correlation analysis indicated that reduced SCFA concentrations in AkTM-allergic mice may be related to decreases in certain SCFA-producing bacteria, such as Clostridia_UCG-014 . The changes in gut microbiota and SCFAs could be partially restored by DAPT treatment. Our findings showed that inhibiting Notch signaling could alleviate AkTM-induced food allergy by correcting Th1/Th2 imbalance and modulating the gut microbiota. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Directory of Open Access Journals: DOAJ Articles Foods 13 8 1144 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antarctic krill tropomyosin food allergy Notch signaling Th1/Th2 imbalance gut microbiota SCFAs Chemical technology TP1-1185 |
spellingShingle |
antarctic krill tropomyosin food allergy Notch signaling Th1/Th2 imbalance gut microbiota SCFAs Chemical technology TP1-1185 Na Lin Hai Chi Quanyou Guo Zhidong Liu Ling Ni Notch Signaling Inhibition Alleviates Allergies Caused by Antarctic Krill Tropomyosin through Improving Th1/Th2 Imbalance and Modulating Gut Microbiota |
topic_facet |
antarctic krill tropomyosin food allergy Notch signaling Th1/Th2 imbalance gut microbiota SCFAs Chemical technology TP1-1185 |
description |
Antarctic krill tropomyosin (AkTM) has been shown in mice to cause IgE-mediated food allergy. The objective of this work was to investigate the role of Notch signaling in AkTM-sensitized mice, as well as to determine the changes in gut microbiota composition and short-chain fatty acids (SCFAs) in the allergic mice. An AkTM-induced food allergy mouse model was built and N -[ N -(3,5-difluorophenacetyl)- L -alanyl]- S -phenylglycine t-butyl ester (DAPT) was used as an γ-secretase inhibitor to inhibit the activation of Notch signaling. Food allergy indices, some key transcription factors, histologic alterations in the small intestine, and changes in gut microbiota composition were examined. The results showed that DAPT inhibited Notch signaling, which reduced AkTM-specific IgE, suppressed mast cell degranulation, decreased IL-4 but increased IFN-γ production, and alleviated allergic symptoms. Quantitative real-time PCR and Western blotting analyses revealed that expressions of Hes-1, Gata3, and IL-4 were down-regulated after DAPT treatment, accompanied by increases in T-bet and IFN-γ, indicating that Notch signaling was active in AkTM-sensitized mice and blocking it could reverse the Th1/Th2 imbalance. Expressions of key transcription factors revealed that Notch signaling could promote Th2 cell differentiation in sensitized mice. Furthermore, 16S rRNA sequencing results revealed that AkTM could alter the diversity and composition of gut microbiota in mice, leading to increases in inflammation-inducing bacteria such as Enterococcus and Escherichia-Shigella . Correlation analysis indicated that reduced SCFA concentrations in AkTM-allergic mice may be related to decreases in certain SCFA-producing bacteria, such as Clostridia_UCG-014 . The changes in gut microbiota and SCFAs could be partially restored by DAPT treatment. Our findings showed that inhibiting Notch signaling could alleviate AkTM-induced food allergy by correcting Th1/Th2 imbalance and modulating the gut microbiota. |
format |
Article in Journal/Newspaper |
author |
Na Lin Hai Chi Quanyou Guo Zhidong Liu Ling Ni |
author_facet |
Na Lin Hai Chi Quanyou Guo Zhidong Liu Ling Ni |
author_sort |
Na Lin |
title |
Notch Signaling Inhibition Alleviates Allergies Caused by Antarctic Krill Tropomyosin through Improving Th1/Th2 Imbalance and Modulating Gut Microbiota |
title_short |
Notch Signaling Inhibition Alleviates Allergies Caused by Antarctic Krill Tropomyosin through Improving Th1/Th2 Imbalance and Modulating Gut Microbiota |
title_full |
Notch Signaling Inhibition Alleviates Allergies Caused by Antarctic Krill Tropomyosin through Improving Th1/Th2 Imbalance and Modulating Gut Microbiota |
title_fullStr |
Notch Signaling Inhibition Alleviates Allergies Caused by Antarctic Krill Tropomyosin through Improving Th1/Th2 Imbalance and Modulating Gut Microbiota |
title_full_unstemmed |
Notch Signaling Inhibition Alleviates Allergies Caused by Antarctic Krill Tropomyosin through Improving Th1/Th2 Imbalance and Modulating Gut Microbiota |
title_sort |
notch signaling inhibition alleviates allergies caused by antarctic krill tropomyosin through improving th1/th2 imbalance and modulating gut microbiota |
publisher |
MDPI AG |
publishDate |
2024 |
url |
https://doi.org/10.3390/foods13081144 https://doaj.org/article/c46166522f3e4a98bca404ce9e845f53 |
genre |
Antarc* Antarctic Antarctic Krill |
genre_facet |
Antarc* Antarctic Antarctic Krill |
op_source |
Foods, Vol 13, Iss 8, p 1144 (2024) |
op_relation |
https://www.mdpi.com/2304-8158/13/8/1144 https://doaj.org/toc/2304-8158 doi:10.3390/foods13081144 2304-8158 https://doaj.org/article/c46166522f3e4a98bca404ce9e845f53 |
op_doi |
https://doi.org/10.3390/foods13081144 |
container_title |
Foods |
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13 |
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8 |
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1144 |
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1810289416566996992 |