Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China
Abstract Background According to China’s Malaria Eradication Action Plan, malaria cases diagnosed and reported by health authorities at the county level must be further re-confirmed by provincial laboratories. The Yunnan Province Malaria Diagnostic Reference Laboratory (YPMDRL) began the synchronous...
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ftdoajarticles:oai:doaj.org/article:c43f5fc51bc641e3aba9760cf1b87df7 2023-05-15T15:18:34+02:00 Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China Ying Dong Yan Deng Yanchun Xu Mengni Chen Chun Wei Canglin Zhang Xianghua Mao Jingbo Xue 2020-11-01T00:00:00Z https://doi.org/10.1186/s12936-020-03477-1 https://doaj.org/article/c43f5fc51bc641e3aba9760cf1b87df7 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03477-1 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03477-1 1475-2875 https://doaj.org/article/c43f5fc51bc641e3aba9760cf1b87df7 Malaria Journal, Vol 19, Iss 1, Pp 1-9 (2020) Yunnan province Malaria cases Diagnosis Re-testing Accuracy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03477-1 2022-12-31T03:32:43Z Abstract Background According to China’s Malaria Eradication Action Plan, malaria cases diagnosed and reported by health authorities at the county level must be further re-confirmed by provincial laboratories. The Yunnan Province Malaria Diagnostic Reference Laboratory (YPMDRL) began the synchronous implementation of microscopic examinations and nested polymerase chain reaction (nested-PCR) testing to re-test the malaria cases initially diagnosed by county-level laboratories and to evaluate the consistency of Plasmodium species identified between by YPMDRL and by the county-level laboratories from 2013 to 2018 in Yunnan Province. Methods Data on malaria initial diagnosis completed by county-level laboratories in Yunnan Province were collected weekly from the “China Disease Prevention and Control Information System” from 2013 to 2018. The YPMDRL performed Plasmodium microscopic examination and 18S rRNA gene nested-PCR testing on every malaria case managed by the China Disease Prevention and Control Information System. The re-testing detection results were fed back to the initial diagnosis and reporting unit for revision of malaria case types. Results A total of 2,869 malaria cases were diagnosed and reported by county-level laboratories in Yunnan Province from 2013 to 2018. The re-testing rate was 95.6% (2,742/2,869), and the re-testing rate increased from 2013 to 2018. Among the re-tested 2,742 cases, 96.7% (2651/2742), 2.2% (59/2742), and 1.1% (32/2742) were doubly examined by microscopy and by nested-PCR, only by microscopy, and only by nested-PCR, respectively. The total Plasmodium species accuracy rate at county-level laboratories was 92.6% (2,543/2,742) reference to the diagnosis by YPMDRL. Among the inconsistent 199 cases, they were identified as including 103 negative cases, 45 falciparum malaria cases, 30 vivax malaria cases, 11 ovale malaria cases, and 10 malariae malaria cases by YPMDRL. From 2013 to 2018, the revised and registered malaria cases by the China Disease Prevention and Control Information ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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English |
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Yunnan province Malaria cases Diagnosis Re-testing Accuracy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Yunnan province Malaria cases Diagnosis Re-testing Accuracy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ying Dong Yan Deng Yanchun Xu Mengni Chen Chun Wei Canglin Zhang Xianghua Mao Jingbo Xue Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China |
topic_facet |
Yunnan province Malaria cases Diagnosis Re-testing Accuracy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background According to China’s Malaria Eradication Action Plan, malaria cases diagnosed and reported by health authorities at the county level must be further re-confirmed by provincial laboratories. The Yunnan Province Malaria Diagnostic Reference Laboratory (YPMDRL) began the synchronous implementation of microscopic examinations and nested polymerase chain reaction (nested-PCR) testing to re-test the malaria cases initially diagnosed by county-level laboratories and to evaluate the consistency of Plasmodium species identified between by YPMDRL and by the county-level laboratories from 2013 to 2018 in Yunnan Province. Methods Data on malaria initial diagnosis completed by county-level laboratories in Yunnan Province were collected weekly from the “China Disease Prevention and Control Information System” from 2013 to 2018. The YPMDRL performed Plasmodium microscopic examination and 18S rRNA gene nested-PCR testing on every malaria case managed by the China Disease Prevention and Control Information System. The re-testing detection results were fed back to the initial diagnosis and reporting unit for revision of malaria case types. Results A total of 2,869 malaria cases were diagnosed and reported by county-level laboratories in Yunnan Province from 2013 to 2018. The re-testing rate was 95.6% (2,742/2,869), and the re-testing rate increased from 2013 to 2018. Among the re-tested 2,742 cases, 96.7% (2651/2742), 2.2% (59/2742), and 1.1% (32/2742) were doubly examined by microscopy and by nested-PCR, only by microscopy, and only by nested-PCR, respectively. The total Plasmodium species accuracy rate at county-level laboratories was 92.6% (2,543/2,742) reference to the diagnosis by YPMDRL. Among the inconsistent 199 cases, they were identified as including 103 negative cases, 45 falciparum malaria cases, 30 vivax malaria cases, 11 ovale malaria cases, and 10 malariae malaria cases by YPMDRL. From 2013 to 2018, the revised and registered malaria cases by the China Disease Prevention and Control Information ... |
format |
Article in Journal/Newspaper |
author |
Ying Dong Yan Deng Yanchun Xu Mengni Chen Chun Wei Canglin Zhang Xianghua Mao Jingbo Xue |
author_facet |
Ying Dong Yan Deng Yanchun Xu Mengni Chen Chun Wei Canglin Zhang Xianghua Mao Jingbo Xue |
author_sort |
Ying Dong |
title |
Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China |
title_short |
Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China |
title_full |
Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China |
title_fullStr |
Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China |
title_full_unstemmed |
Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China |
title_sort |
analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in yunnan province, china |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03477-1 https://doaj.org/article/c43f5fc51bc641e3aba9760cf1b87df7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-9 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03477-1 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03477-1 1475-2875 https://doaj.org/article/c43f5fc51bc641e3aba9760cf1b87df7 |
op_doi |
https://doi.org/10.1186/s12936-020-03477-1 |
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Malaria Journal |
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19 |
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1 |
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1766348756656062464 |