Molecular characterization of Echinococcus granulosus in paraffin-embedded human tissues from Southwest Iran
Objective: To investigate Echinococcus (E.) granulosus genotypes as the causative agents of hydatidosis in humans in the southwest of Iran (Khuzestan province). Methods: In this study, isolates of 80 archived human paraffin embedded hydatid cysts were collected from pathology laboratories in Ahvaz c...
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ftdoajarticles:oai:doaj.org/article:c42cf99524f34d919147725035b8b3d9 2023-05-15T15:07:20+02:00 Molecular characterization of Echinococcus granulosus in paraffin-embedded human tissues from Southwest Iran Elham Yousefi Abdollah Rafiei Iran Rashidi Shahram Khademvatan Masoud Foroutan 2019-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.271290 https://doaj.org/article/c42cf99524f34d919147725035b8b3d9 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=11;spage=507;epage=511;aulast=Yousefi https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.271290 https://doaj.org/article/c42cf99524f34d919147725035b8b3d9 Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 11, Pp 507-511 (2019) echinococcus granulosus human genotype iran Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.4103/1995-7645.271290 2022-12-31T16:11:16Z Objective: To investigate Echinococcus (E.) granulosus genotypes as the causative agents of hydatidosis in humans in the southwest of Iran (Khuzestan province). Methods: In this study, isolates of 80 archived human paraffin embedded hydatid cysts were collected from pathology laboratories in Ahvaz city, Khuzestan province. DNA was extracted and examined by nested-PCR of ribosomal DNA (rDNA) internal transcribed spacer 1 (ITS1), and PCR-RFLP. In addition, the sequences of fragments of genes coding for Cox space1 and NADH dehydrogenase 1 (ND1) were also examined. Results: Of the 80 paraffin samples, 44 (55.0%) were from the liver, 27 (33.8%) from the lung, and the rest from other organs. The amplified hydatid genomic DNA showed that the cysts were E. granulosus strains. The results of PCR-RFLP and sequencing analysis revealed the presence of G1 genotype (sheep strain) in all human isolates. Furthermore, no camel strain (G6) was detected among all samples in the regions studied. Conclusions: The molecular findings indicate that the predominant genotype involved in E. granulosus transmission in southwest of Iran is the common sheep strain (G1), which occurs in human populations. These results may have important implications for hydatid disease control in the studied areas. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 12 11 507 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
echinococcus granulosus human genotype iran Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
echinococcus granulosus human genotype iran Arctic medicine. Tropical medicine RC955-962 Elham Yousefi Abdollah Rafiei Iran Rashidi Shahram Khademvatan Masoud Foroutan Molecular characterization of Echinococcus granulosus in paraffin-embedded human tissues from Southwest Iran |
topic_facet |
echinococcus granulosus human genotype iran Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate Echinococcus (E.) granulosus genotypes as the causative agents of hydatidosis in humans in the southwest of Iran (Khuzestan province). Methods: In this study, isolates of 80 archived human paraffin embedded hydatid cysts were collected from pathology laboratories in Ahvaz city, Khuzestan province. DNA was extracted and examined by nested-PCR of ribosomal DNA (rDNA) internal transcribed spacer 1 (ITS1), and PCR-RFLP. In addition, the sequences of fragments of genes coding for Cox space1 and NADH dehydrogenase 1 (ND1) were also examined. Results: Of the 80 paraffin samples, 44 (55.0%) were from the liver, 27 (33.8%) from the lung, and the rest from other organs. The amplified hydatid genomic DNA showed that the cysts were E. granulosus strains. The results of PCR-RFLP and sequencing analysis revealed the presence of G1 genotype (sheep strain) in all human isolates. Furthermore, no camel strain (G6) was detected among all samples in the regions studied. Conclusions: The molecular findings indicate that the predominant genotype involved in E. granulosus transmission in southwest of Iran is the common sheep strain (G1), which occurs in human populations. These results may have important implications for hydatid disease control in the studied areas. |
format |
Article in Journal/Newspaper |
author |
Elham Yousefi Abdollah Rafiei Iran Rashidi Shahram Khademvatan Masoud Foroutan |
author_facet |
Elham Yousefi Abdollah Rafiei Iran Rashidi Shahram Khademvatan Masoud Foroutan |
author_sort |
Elham Yousefi |
title |
Molecular characterization of Echinococcus granulosus in paraffin-embedded human tissues from Southwest Iran |
title_short |
Molecular characterization of Echinococcus granulosus in paraffin-embedded human tissues from Southwest Iran |
title_full |
Molecular characterization of Echinococcus granulosus in paraffin-embedded human tissues from Southwest Iran |
title_fullStr |
Molecular characterization of Echinococcus granulosus in paraffin-embedded human tissues from Southwest Iran |
title_full_unstemmed |
Molecular characterization of Echinococcus granulosus in paraffin-embedded human tissues from Southwest Iran |
title_sort |
molecular characterization of echinococcus granulosus in paraffin-embedded human tissues from southwest iran |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/1995-7645.271290 https://doaj.org/article/c42cf99524f34d919147725035b8b3d9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 11, Pp 507-511 (2019) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=11;spage=507;epage=511;aulast=Yousefi https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.271290 https://doaj.org/article/c42cf99524f34d919147725035b8b3d9 |
op_doi |
https://doi.org/10.4103/1995-7645.271290 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
12 |
container_issue |
11 |
container_start_page |
507 |
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1766338866382372864 |