Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection
Abstract Background Thirty-one glycosylphosphatidylinositol (GPI)-anchored proteins of Plasmodium vivax, merozoite surface protein 1 (MSP1), MSP1 paralogue, MSP4, MSP5, MSP8, and MSP10 have been reported from homologs of Plasmodium falciparum by gene annotation with bioinformatics tools. These GPI-a...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-017-1837-5 https://doaj.org/article/c4252c00a1004e2488833bd550477c6b |
id |
ftdoajarticles:oai:doaj.org/article:c4252c00a1004e2488833bd550477c6b |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:c4252c00a1004e2488833bd550477c6b 2023-05-15T15:17:09+02:00 Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection Yang Cheng Bo Wang Siriruk Changrob Jin-Hee Han Jetsumon Sattabongkot Kwon-Soo Ha Patchanee Chootong Feng Lu Jun Cao Myat Htut Nyunt Won Sun Park Seok-Ho Hong Chae Seung Lim Takafumi Tsuboi Eun-Taek Han 2017-05-01T00:00:00Z https://doi.org/10.1186/s12936-017-1837-5 https://doaj.org/article/c4252c00a1004e2488833bd550477c6b EN eng BMC http://link.springer.com/article/10.1186/s12936-017-1837-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1837-5 1475-2875 https://doaj.org/article/c4252c00a1004e2488833bd550477c6b Malaria Journal, Vol 16, Iss 1, Pp 1-12 (2017) Plasmodium vivax Merozoite surface protein 8 Immunogenicity Food vacuole Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-1837-5 2022-12-31T00:59:27Z Abstract Background Thirty-one glycosylphosphatidylinositol (GPI)-anchored proteins of Plasmodium vivax, merozoite surface protein 1 (MSP1), MSP1 paralogue, MSP4, MSP5, MSP8, and MSP10 have been reported from homologs of Plasmodium falciparum by gene annotation with bioinformatics tools. These GPI-anchored proteins contain two epidermal growth factor (EGF)-like domains at its C-terminus. Here, P. vivax merozoite surface protein 8 (PvMSP8) are considered as potential targets of protective immunity. Methods Recombinant PvMSP8 (rPvMSP8) was expressed, purified, and used for the assessment of humoral and cellular immune responses in P. vivax-infected patients and immune mice. Moreover, the target epitope of ant-PvMSP8 antibodies and subcellular localization of PvMSP8 was also determined. Results The rPvMSP8 was successfully expressed and purified as soluble form as ~55 kDa. PvMSP8 was localized to the outer circle of pigments associated with the food vacuole. The rPvMSP8 protein had a high antigenicity (73.2% in sensitivity and 96.2% in specificity) in patients infected with P. vivax. IgG2 antibody subtype was the predominantly responses to this antigen. Antibody response to PvMSP8 increased up to day 7 and after that slightly decreased within a month. The longevity of anti-PvMSP8 antibody was stably sustained up to 12-year recovery patient samples. Most anti-PvMSP8 antibodies recognized two epitopes that were located outside the C-terminal EGF-like domain. The cellular immune response in P. vivax-exposed individuals produced high levels of IFN-γ and IL-10 upon PvMSP8 antigen stimulation in vitro. Conclusions All data in this study suggest that PvMSP8 antigen has a potential to induce both humoral and cellular immune responses in patients with P. vivax infection. The subcellular localization of PvMSP8 confirmed that it was associated with the parasite food vacuole in blood-stage parasites. A further characterization of this protein will be useful for blood stage P. vivax vaccine development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plasmodium vivax Merozoite surface protein 8 Immunogenicity Food vacuole Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium vivax Merozoite surface protein 8 Immunogenicity Food vacuole Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Yang Cheng Bo Wang Siriruk Changrob Jin-Hee Han Jetsumon Sattabongkot Kwon-Soo Ha Patchanee Chootong Feng Lu Jun Cao Myat Htut Nyunt Won Sun Park Seok-Ho Hong Chae Seung Lim Takafumi Tsuboi Eun-Taek Han Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection |
topic_facet |
Plasmodium vivax Merozoite surface protein 8 Immunogenicity Food vacuole Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Thirty-one glycosylphosphatidylinositol (GPI)-anchored proteins of Plasmodium vivax, merozoite surface protein 1 (MSP1), MSP1 paralogue, MSP4, MSP5, MSP8, and MSP10 have been reported from homologs of Plasmodium falciparum by gene annotation with bioinformatics tools. These GPI-anchored proteins contain two epidermal growth factor (EGF)-like domains at its C-terminus. Here, P. vivax merozoite surface protein 8 (PvMSP8) are considered as potential targets of protective immunity. Methods Recombinant PvMSP8 (rPvMSP8) was expressed, purified, and used for the assessment of humoral and cellular immune responses in P. vivax-infected patients and immune mice. Moreover, the target epitope of ant-PvMSP8 antibodies and subcellular localization of PvMSP8 was also determined. Results The rPvMSP8 was successfully expressed and purified as soluble form as ~55 kDa. PvMSP8 was localized to the outer circle of pigments associated with the food vacuole. The rPvMSP8 protein had a high antigenicity (73.2% in sensitivity and 96.2% in specificity) in patients infected with P. vivax. IgG2 antibody subtype was the predominantly responses to this antigen. Antibody response to PvMSP8 increased up to day 7 and after that slightly decreased within a month. The longevity of anti-PvMSP8 antibody was stably sustained up to 12-year recovery patient samples. Most anti-PvMSP8 antibodies recognized two epitopes that were located outside the C-terminal EGF-like domain. The cellular immune response in P. vivax-exposed individuals produced high levels of IFN-γ and IL-10 upon PvMSP8 antigen stimulation in vitro. Conclusions All data in this study suggest that PvMSP8 antigen has a potential to induce both humoral and cellular immune responses in patients with P. vivax infection. The subcellular localization of PvMSP8 confirmed that it was associated with the parasite food vacuole in blood-stage parasites. A further characterization of this protein will be useful for blood stage P. vivax vaccine development. |
format |
Article in Journal/Newspaper |
author |
Yang Cheng Bo Wang Siriruk Changrob Jin-Hee Han Jetsumon Sattabongkot Kwon-Soo Ha Patchanee Chootong Feng Lu Jun Cao Myat Htut Nyunt Won Sun Park Seok-Ho Hong Chae Seung Lim Takafumi Tsuboi Eun-Taek Han |
author_facet |
Yang Cheng Bo Wang Siriruk Changrob Jin-Hee Han Jetsumon Sattabongkot Kwon-Soo Ha Patchanee Chootong Feng Lu Jun Cao Myat Htut Nyunt Won Sun Park Seok-Ho Hong Chae Seung Lim Takafumi Tsuboi Eun-Taek Han |
author_sort |
Yang Cheng |
title |
Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection |
title_short |
Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection |
title_full |
Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection |
title_fullStr |
Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection |
title_full_unstemmed |
Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection |
title_sort |
naturally acquired humoral and cellular immune responses to plasmodium vivax merozoite surface protein 8 in patients with p. vivax infection |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-1837-5 https://doaj.org/article/c4252c00a1004e2488833bd550477c6b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-12 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-1837-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1837-5 1475-2875 https://doaj.org/article/c4252c00a1004e2488833bd550477c6b |
op_doi |
https://doi.org/10.1186/s12936-017-1837-5 |
container_title |
Malaria Journal |
container_volume |
16 |
container_issue |
1 |
_version_ |
1766347416585371648 |