Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes
Background: In fish farming, the plant extracts containing antioxidant compounds have been added to the diet for enhancing pathogen resistance. In vitro studies evaluating the antioxidant effect of herbal extracts on fish cell models have focused on ROS production and the respiratory burst mechanism...
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2021
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Online Access: | https://doi.org/10.1016/j.ejbt.2021.04.004 https://doaj.org/article/c3e7d01c9c9c40e6a3e0b69477147c6a |
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ftdoajarticles:oai:doaj.org/article:c3e7d01c9c9c40e6a3e0b69477147c6a 2023-05-15T15:32:50+02:00 Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes Paula Andrea Santana Carlos Jara-Gutiérrez Marco Mellado Juan Carlos Forero Fanny Guzmán Andrés Barriga Fernando Albericio Claudio Andrés Álvarez 2021-07-01T00:00:00Z https://doi.org/10.1016/j.ejbt.2021.04.004 https://doaj.org/article/c3e7d01c9c9c40e6a3e0b69477147c6a EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S0717345821000191 https://doaj.org/toc/0717-3458 0717-3458 doi:10.1016/j.ejbt.2021.04.004 https://doaj.org/article/c3e7d01c9c9c40e6a3e0b69477147c6a Electronic Journal of Biotechnology, Vol 52, Iss , Pp 13-20 (2021) Atlantic salmon (Salmo salar) Elderflower polyphenol Enzymatic antioxidant defense Fish aquaculture Fish farming Oxidative stress Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.1016/j.ejbt.2021.04.004 2022-12-31T12:42:15Z Background: In fish farming, the plant extracts containing antioxidant compounds have been added to the diet for enhancing pathogen resistance. In vitro studies evaluating the antioxidant effect of herbal extracts on fish cell models have focused on ROS production and the respiratory burst mechanism. However, the effects on enzymatic antioxidant defense on salmon leukocytes have not been evaluated. This study aims to evaluate the enzymatic antioxidant defense and ROS-induced cell damage in Salmon Head Kidney-1 (SHK-1) cell line exposed to polyphenol-enriched extract from Sambucus nigra flowers. Results: Firstly, the Total Reactive Antioxidant Power (TRAP) assay of elderflower polyphenol (EP) was evaluated, showing 459 and 489 times more active than gallic acid and butyl hydroxy toluene (BHT), respectively. The toxic effect of EP on salmon cells was not significant at concentrations below 120 µg/mL and no hemolysis activity was observed between 20 and 400 µg/mL. The treatment of SHK-1 cell line with EP decreased both the lipid peroxidation and protein oxidation induced by H2O2, which could be associated with decreasing oxidative stress in the SHK-1 cells since the GSH/GSSG ratio increased when only EP was added. Conclusions: These results suggest that plant extracts enriched with polyphenols could improve the enzymatic antioxidant defense of salmon leukocytes and protect the cells against ROS-induced cell damage.How to cite: Santana PA, Jara-Gutiérrez C, Mellado M, et al. Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.04.004 Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Gutiérrez ENVELOPE(-57.917,-57.917,-63.300,-63.300) Electronic Journal of Biotechnology 52 13 20 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic salmon (Salmo salar) Elderflower polyphenol Enzymatic antioxidant defense Fish aquaculture Fish farming Oxidative stress Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 |
spellingShingle |
Atlantic salmon (Salmo salar) Elderflower polyphenol Enzymatic antioxidant defense Fish aquaculture Fish farming Oxidative stress Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 Paula Andrea Santana Carlos Jara-Gutiérrez Marco Mellado Juan Carlos Forero Fanny Guzmán Andrés Barriga Fernando Albericio Claudio Andrés Álvarez Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes |
topic_facet |
Atlantic salmon (Salmo salar) Elderflower polyphenol Enzymatic antioxidant defense Fish aquaculture Fish farming Oxidative stress Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 |
description |
Background: In fish farming, the plant extracts containing antioxidant compounds have been added to the diet for enhancing pathogen resistance. In vitro studies evaluating the antioxidant effect of herbal extracts on fish cell models have focused on ROS production and the respiratory burst mechanism. However, the effects on enzymatic antioxidant defense on salmon leukocytes have not been evaluated. This study aims to evaluate the enzymatic antioxidant defense and ROS-induced cell damage in Salmon Head Kidney-1 (SHK-1) cell line exposed to polyphenol-enriched extract from Sambucus nigra flowers. Results: Firstly, the Total Reactive Antioxidant Power (TRAP) assay of elderflower polyphenol (EP) was evaluated, showing 459 and 489 times more active than gallic acid and butyl hydroxy toluene (BHT), respectively. The toxic effect of EP on salmon cells was not significant at concentrations below 120 µg/mL and no hemolysis activity was observed between 20 and 400 µg/mL. The treatment of SHK-1 cell line with EP decreased both the lipid peroxidation and protein oxidation induced by H2O2, which could be associated with decreasing oxidative stress in the SHK-1 cells since the GSH/GSSG ratio increased when only EP was added. Conclusions: These results suggest that plant extracts enriched with polyphenols could improve the enzymatic antioxidant defense of salmon leukocytes and protect the cells against ROS-induced cell damage.How to cite: Santana PA, Jara-Gutiérrez C, Mellado M, et al. Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.04.004 |
format |
Article in Journal/Newspaper |
author |
Paula Andrea Santana Carlos Jara-Gutiérrez Marco Mellado Juan Carlos Forero Fanny Guzmán Andrés Barriga Fernando Albericio Claudio Andrés Álvarez |
author_facet |
Paula Andrea Santana Carlos Jara-Gutiérrez Marco Mellado Juan Carlos Forero Fanny Guzmán Andrés Barriga Fernando Albericio Claudio Andrés Álvarez |
author_sort |
Paula Andrea Santana |
title |
Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes |
title_short |
Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes |
title_full |
Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes |
title_fullStr |
Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes |
title_full_unstemmed |
Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes |
title_sort |
effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doi.org/10.1016/j.ejbt.2021.04.004 https://doaj.org/article/c3e7d01c9c9c40e6a3e0b69477147c6a |
long_lat |
ENVELOPE(-57.917,-57.917,-63.300,-63.300) |
geographic |
Gutiérrez |
geographic_facet |
Gutiérrez |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Electronic Journal of Biotechnology, Vol 52, Iss , Pp 13-20 (2021) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S0717345821000191 https://doaj.org/toc/0717-3458 0717-3458 doi:10.1016/j.ejbt.2021.04.004 https://doaj.org/article/c3e7d01c9c9c40e6a3e0b69477147c6a |
op_doi |
https://doi.org/10.1016/j.ejbt.2021.04.004 |
container_title |
Electronic Journal of Biotechnology |
container_volume |
52 |
container_start_page |
13 |
op_container_end_page |
20 |
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1766363317141504000 |