Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana
Abstract Background Accurate diagnosis and timely treatment are crucial in combating malaria. Methods A total of 449 samples were screened for Plasmodium falciparum infection by expert microscopy, qPCR, and three RDTs, namely Rapigen Biocredit Malaria Ag Pf (detecting HRP2 and pLDH on separate bands...
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ftdoajarticles:oai:doaj.org/article:c3cb2e9340eb4b319cc34ff0c1f4ab1e 2024-09-30T14:31:42+00:00 Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana Tolulope Adeyemi Kayode Agyapong Kofi Addo Thomas Kwame Addison Austine Tweneboah Stephen Opoku Afriyie Dawood Ackom Abbas Ayesha Seth Abraham K. Badu-Tawiah Kingsley Badu Cristian Koepfli 2024-08-01T00:00:00Z https://doi.org/10.1186/s12936-024-05073-z https://doaj.org/article/c3cb2e9340eb4b319cc34ff0c1f4ab1e EN eng BMC https://doi.org/10.1186/s12936-024-05073-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-024-05073-z 1475-2875 https://doaj.org/article/c3cb2e9340eb4b319cc34ff0c1f4ab1e Malaria Journal, Vol 23, Iss 1, Pp 1-7 (2024) Malaria diagnosis Rapid diagnostic test Ultrasensitive RDT HRP2 PLDH Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2024 ftdoajarticles https://doi.org/10.1186/s12936-024-05073-z 2024-09-02T15:34:35Z Abstract Background Accurate diagnosis and timely treatment are crucial in combating malaria. Methods A total of 449 samples were screened for Plasmodium falciparum infection by expert microscopy, qPCR, and three RDTs, namely Rapigen Biocredit Malaria Ag Pf (detecting HRP2 and pLDH on separate bands), Abbott NxTek Eliminate Malaria Ag Pf (detecting HRP2), and SD Bioline Malaria Ag Pf (detecting HRP2). hrp2/3 deletion typing was done by digital PCR. Results 45.7% (205/449) individuals tested positive by qPCR for P. falciparum with a mean parasite density of 12.5 parasites/μL. Using qPCR as reference, the sensitivity of microscopy was 28.3% (58/205), the Biocredit RDT was 52.2% (107/205), the NxTek RDT was 49.3% (101/205), and the Bioline RDT was 39.5% (81/205). When only samples with densities > 20 parasites/μL were included (n = 89), sensitivity of 62.9% (56/89) by microscopy, 88.8% (79/89) by Biocredit, 88.8% (79/89) by NxTek, and 78.7% (70/89) by Bioline were obtained. All three RDTs demonstrated specificities > 95%. The limits of detection (95% probability that a sample tested positive) was 4393 parasites/μL (microscopy), 56 parasites/μL (Biocredit, considering either HRP2 or pLDH), 84 parasites/μL (NxTek), and 331 parasites/μL (Bioline). None of the three qPCR-confirmed P. falciparum positive samples, identified solely through the pLDH target, or eight samples negative for all RDTs but qPCR-positive at densities > 20 parasites/µL carried hrp2/3 deletions. Conclusion The Biocredit and NxTek RDTs demonstrated comparable diagnostic efficacies. All three RDTs performed better than microscopy. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Abbott ENVELOPE(-62.133,-62.133,-64.100,-64.100) Arctic Malaria Journal 23 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria diagnosis Rapid diagnostic test Ultrasensitive RDT HRP2 PLDH Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria diagnosis Rapid diagnostic test Ultrasensitive RDT HRP2 PLDH Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Tolulope Adeyemi Kayode Agyapong Kofi Addo Thomas Kwame Addison Austine Tweneboah Stephen Opoku Afriyie Dawood Ackom Abbas Ayesha Seth Abraham K. Badu-Tawiah Kingsley Badu Cristian Koepfli Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana |
topic_facet |
Malaria diagnosis Rapid diagnostic test Ultrasensitive RDT HRP2 PLDH Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Accurate diagnosis and timely treatment are crucial in combating malaria. Methods A total of 449 samples were screened for Plasmodium falciparum infection by expert microscopy, qPCR, and three RDTs, namely Rapigen Biocredit Malaria Ag Pf (detecting HRP2 and pLDH on separate bands), Abbott NxTek Eliminate Malaria Ag Pf (detecting HRP2), and SD Bioline Malaria Ag Pf (detecting HRP2). hrp2/3 deletion typing was done by digital PCR. Results 45.7% (205/449) individuals tested positive by qPCR for P. falciparum with a mean parasite density of 12.5 parasites/μL. Using qPCR as reference, the sensitivity of microscopy was 28.3% (58/205), the Biocredit RDT was 52.2% (107/205), the NxTek RDT was 49.3% (101/205), and the Bioline RDT was 39.5% (81/205). When only samples with densities > 20 parasites/μL were included (n = 89), sensitivity of 62.9% (56/89) by microscopy, 88.8% (79/89) by Biocredit, 88.8% (79/89) by NxTek, and 78.7% (70/89) by Bioline were obtained. All three RDTs demonstrated specificities > 95%. The limits of detection (95% probability that a sample tested positive) was 4393 parasites/μL (microscopy), 56 parasites/μL (Biocredit, considering either HRP2 or pLDH), 84 parasites/μL (NxTek), and 331 parasites/μL (Bioline). None of the three qPCR-confirmed P. falciparum positive samples, identified solely through the pLDH target, or eight samples negative for all RDTs but qPCR-positive at densities > 20 parasites/µL carried hrp2/3 deletions. Conclusion The Biocredit and NxTek RDTs demonstrated comparable diagnostic efficacies. All three RDTs performed better than microscopy. |
format |
Article in Journal/Newspaper |
author |
Tolulope Adeyemi Kayode Agyapong Kofi Addo Thomas Kwame Addison Austine Tweneboah Stephen Opoku Afriyie Dawood Ackom Abbas Ayesha Seth Abraham K. Badu-Tawiah Kingsley Badu Cristian Koepfli |
author_facet |
Tolulope Adeyemi Kayode Agyapong Kofi Addo Thomas Kwame Addison Austine Tweneboah Stephen Opoku Afriyie Dawood Ackom Abbas Ayesha Seth Abraham K. Badu-Tawiah Kingsley Badu Cristian Koepfli |
author_sort |
Tolulope Adeyemi Kayode |
title |
Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana |
title_short |
Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana |
title_full |
Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana |
title_fullStr |
Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana |
title_full_unstemmed |
Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana |
title_sort |
comparison of three rapid diagnostic tests for plasmodium falciparum diagnosis in ghana |
publisher |
BMC |
publishDate |
2024 |
url |
https://doi.org/10.1186/s12936-024-05073-z https://doaj.org/article/c3cb2e9340eb4b319cc34ff0c1f4ab1e |
long_lat |
ENVELOPE(-62.133,-62.133,-64.100,-64.100) |
geographic |
Abbott Arctic |
geographic_facet |
Abbott Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 23, Iss 1, Pp 1-7 (2024) |
op_relation |
https://doi.org/10.1186/s12936-024-05073-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-024-05073-z 1475-2875 https://doaj.org/article/c3cb2e9340eb4b319cc34ff0c1f4ab1e |
op_doi |
https://doi.org/10.1186/s12936-024-05073-z |
container_title |
Malaria Journal |
container_volume |
23 |
container_issue |
1 |
_version_ |
1811636112651714560 |