Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area
INTRODUCTION: The laboratory diagnosis of schistosomiasis is based mainly on the detection of parasite eggs in stool samples through the Kato-Katz (KK) technique, reading one slide by test. However, a widely known limitation of parasitological methods is reduced sensitivity, particularly in low ende...
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ftdoajarticles:oai:doaj.org/article:c3af858bf8b149d49c6b8e096cf9c75e 2023-05-15T15:09:33+02:00 Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area Teiliane Rodrigues Carneiro Marta Cristhiany Cunha Pinheiro Sara Menezes de Oliveira Ana Lúcia de Paula Hanemann José Ajax Nogueira Queiroz Fernando Schemelzer Moraes Bezerra 2012-08-01T00:00:00Z https://doi.org/10.1590/S0037-86822012000400019 https://doaj.org/article/c3af858bf8b149d49c6b8e096cf9c75e EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400019&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000400019 https://doaj.org/article/c3af858bf8b149d49c6b8e096cf9c75e Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 4, Pp 510-513 (2012) Esquistossomose Diagnóstico Schistosoma mansoni Kato-Katz ELISA Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1590/S0037-86822012000400019 2022-12-30T21:39:23Z INTRODUCTION: The laboratory diagnosis of schistosomiasis is based mainly on the detection of parasite eggs in stool samples through the Kato-Katz (KK) technique, reading one slide by test. However, a widely known limitation of parasitological methods is reduced sensitivity, particularly in low endemic areas. METHODS: To increase sensitivity, we conducted further slide readings from the same stool sample using the parasitological method associated with a serological test. We used the KK method (three slides) and the IgG anti-Schistosoma mansoni-enzyme-linked immunosorbent assay (ELISA) technique to diagnose schistosomiasis in low endemic areas in the Brazilian State of Ceará. Fecal samples and sera from 250 individuals were analyzed. RESULTS: Sixteen percent and 47.2% of samples were positive in parasitological tests and serological tests, respectively. Parasitological methods showed that 32 (80%) individuals tested positive on the first slide, 6 (15%) on the second slide, and 2 (5%) on the third. The performance of the ELISA test in the diagnosis, using the KK method as diagnostic reference, showed a negative predictive value of 100%, with specificity and positive predictive values of 62.8% and 33.9%, respectively. CONCLUSIONS: In this study, the increase from one to three slides analyzed per sample using the KK technique was shown to be a useful procedure for increasing the diagnostic sensitivity of this technique. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 45 4 510 513 |
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Esquistossomose Diagnóstico Schistosoma mansoni Kato-Katz ELISA Arctic medicine. Tropical medicine RC955-962 |
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Esquistossomose Diagnóstico Schistosoma mansoni Kato-Katz ELISA Arctic medicine. Tropical medicine RC955-962 Teiliane Rodrigues Carneiro Marta Cristhiany Cunha Pinheiro Sara Menezes de Oliveira Ana Lúcia de Paula Hanemann José Ajax Nogueira Queiroz Fernando Schemelzer Moraes Bezerra Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area |
topic_facet |
Esquistossomose Diagnóstico Schistosoma mansoni Kato-Katz ELISA Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: The laboratory diagnosis of schistosomiasis is based mainly on the detection of parasite eggs in stool samples through the Kato-Katz (KK) technique, reading one slide by test. However, a widely known limitation of parasitological methods is reduced sensitivity, particularly in low endemic areas. METHODS: To increase sensitivity, we conducted further slide readings from the same stool sample using the parasitological method associated with a serological test. We used the KK method (three slides) and the IgG anti-Schistosoma mansoni-enzyme-linked immunosorbent assay (ELISA) technique to diagnose schistosomiasis in low endemic areas in the Brazilian State of Ceará. Fecal samples and sera from 250 individuals were analyzed. RESULTS: Sixteen percent and 47.2% of samples were positive in parasitological tests and serological tests, respectively. Parasitological methods showed that 32 (80%) individuals tested positive on the first slide, 6 (15%) on the second slide, and 2 (5%) on the third. The performance of the ELISA test in the diagnosis, using the KK method as diagnostic reference, showed a negative predictive value of 100%, with specificity and positive predictive values of 62.8% and 33.9%, respectively. CONCLUSIONS: In this study, the increase from one to three slides analyzed per sample using the KK technique was shown to be a useful procedure for increasing the diagnostic sensitivity of this technique. |
format |
Article in Journal/Newspaper |
author |
Teiliane Rodrigues Carneiro Marta Cristhiany Cunha Pinheiro Sara Menezes de Oliveira Ana Lúcia de Paula Hanemann José Ajax Nogueira Queiroz Fernando Schemelzer Moraes Bezerra |
author_facet |
Teiliane Rodrigues Carneiro Marta Cristhiany Cunha Pinheiro Sara Menezes de Oliveira Ana Lúcia de Paula Hanemann José Ajax Nogueira Queiroz Fernando Schemelzer Moraes Bezerra |
author_sort |
Teiliane Rodrigues Carneiro |
title |
Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area |
title_short |
Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area |
title_full |
Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area |
title_fullStr |
Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area |
title_full_unstemmed |
Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area |
title_sort |
increased detection of schistosomiasis with kato-katz and swap-igg-elisa in a northeastern brazil low-intensity transmission area |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0037-86822012000400019 https://doaj.org/article/c3af858bf8b149d49c6b8e096cf9c75e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 4, Pp 510-513 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400019&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000400019 https://doaj.org/article/c3af858bf8b149d49c6b8e096cf9c75e |
op_doi |
https://doi.org/10.1590/S0037-86822012000400019 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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45 |
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