INVESTIGATION OF BIOFILM FORMATION IN COAGULASE-NEGATIVE STAPHYLOCOCCI ISOLATED FROM PLATELET CONCENTRATE BAGS

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Rosiéli MARTINI, Rosmari HÖRNER, Roberta Filipini RAMPELOTTO, Litiérri Razia Litiérri GARZON, Melise Silveira NUNES, Mayza Dalcin TEIXEIRA, Daniel Ângelo Sganzerla GRAICHEN
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2016
• Subjects: Biofilm; Staphylococci; Platelet Concentrates; Transfusion; Microbiology; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S1678-9946201658001; https://doaj.org/article/c3aa3293d1ff4cb0918a9fd5e2648b7c

Platelet Concentrates (PCs) are the blood components with the highest rate of bacterial contamination, and coagulase-negative staphylococci (CoNS) are the most frequently isolated contaminants. This study investigated the biofilm formation of 16 contaminated units out of 691 PCs tested by phenotypic and genotypic methods. Adhesion in Borosilicate Tube (ABT) and Congo Red Agar (CRA) tests were used to assess the presence of biofilm. The presence of icaADC genes was assessed by means of the Polymerase Chain Reaction (PCR) technique. With Vitek(r)2, Staphylococcus haemolyticus was considered the most prevalent CoNS (31.25%). The CRA characterized 43.8% as probable biofilm producers, and for the ABT test, 37.5%. The icaADC genes were identified in seven samples by the PCR. The ABT technique showed 85.7% sensitivity and 100% specificity when compared to the reference method (PCR), and presented strong agreement (k = 0.8). This study shows that species identified as PCs contaminants are considered inhabitants of the normal skin flora and they might become important pathogens. The results also lead to the recommendation of ABT use in laboratory routine for detecting biofilm in CoNS contaminants of PCs.