Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.
Background Childhood diarrhoea, a major cause of morbidity and mortality in low-income regions, remains scarcely studied in many countries, such as Guinea-Bissau. Stool sample drying enables later qPCR analyses of pathogens without concern about electricity shortages. Methods Dried stool samples of...
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ftdoajarticles:oai:doaj.org/article:c3a52d288cb4492b99babd02e8a60a6c 2023-05-15T15:09:51+02:00 Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau. Sointu Mero Suvi Timonen Tinja Lääveri Sandra Løfberg Juha Kirveskari Johan Ursing Lars Rombo Poul-Erik Kofoed Anu Kantele 2021-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009709 https://doaj.org/article/c3a52d288cb4492b99babd02e8a60a6c EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009709 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009709 https://doaj.org/article/c3a52d288cb4492b99babd02e8a60a6c PLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009709 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009709 2022-12-31T15:16:56Z Background Childhood diarrhoea, a major cause of morbidity and mortality in low-income regions, remains scarcely studied in many countries, such as Guinea-Bissau. Stool sample drying enables later qPCR analyses of pathogens without concern about electricity shortages. Methods Dried stool samples of children under five years treated at the Bandim Health Centre in Bissau, Guinea-Bissau were screened by qPCR for nine enteric bacteria, five viruses, and four parasites. The findings of children having and not having diarrhoea were compared in age groups 0-11 and 12-59 months. Results Of the 429 children- 228 with and 201 without diarrhoea- 96.9% and 93.5% had bacterial, 62.7% and 44.3% viral, and 52.6% and 48.3% parasitic pathogen findings, respectively. Enteroaggregarive Escherichia coli (EAEC; 60.5% versus 66.7%), enteropathogenic E. coli (EPEC; 61.4% versus 62.7%), Campylobacter (53.2% versus 51.8%), and enterotoxigenic E. coli (ETEC; 54.4% versus 44.3%) were the most common bacterial pathogens. Diarrhoea was associated with enteroinvasive E. coli (EIEC)/Shigella (63.3%), ETEC (54.4%), astrovirus (75.0%), norovirus GII (72.6%) and Cryptosporidium (71.2%). The only pathogen associated with severe diarrhoea was EIEC/Shigella (p<0.001). EAEC was found more frequent among the infants, and EIEC/Shigella, Giardia duodenalis and Dientamoeba fragilis among the older children. Conclusions Stool pathogens proved common among all the children regardless of them having diarrhoea or not. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 9 e0009709 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sointu Mero Suvi Timonen Tinja Lääveri Sandra Løfberg Juha Kirveskari Johan Ursing Lars Rombo Poul-Erik Kofoed Anu Kantele Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Childhood diarrhoea, a major cause of morbidity and mortality in low-income regions, remains scarcely studied in many countries, such as Guinea-Bissau. Stool sample drying enables later qPCR analyses of pathogens without concern about electricity shortages. Methods Dried stool samples of children under five years treated at the Bandim Health Centre in Bissau, Guinea-Bissau were screened by qPCR for nine enteric bacteria, five viruses, and four parasites. The findings of children having and not having diarrhoea were compared in age groups 0-11 and 12-59 months. Results Of the 429 children- 228 with and 201 without diarrhoea- 96.9% and 93.5% had bacterial, 62.7% and 44.3% viral, and 52.6% and 48.3% parasitic pathogen findings, respectively. Enteroaggregarive Escherichia coli (EAEC; 60.5% versus 66.7%), enteropathogenic E. coli (EPEC; 61.4% versus 62.7%), Campylobacter (53.2% versus 51.8%), and enterotoxigenic E. coli (ETEC; 54.4% versus 44.3%) were the most common bacterial pathogens. Diarrhoea was associated with enteroinvasive E. coli (EIEC)/Shigella (63.3%), ETEC (54.4%), astrovirus (75.0%), norovirus GII (72.6%) and Cryptosporidium (71.2%). The only pathogen associated with severe diarrhoea was EIEC/Shigella (p<0.001). EAEC was found more frequent among the infants, and EIEC/Shigella, Giardia duodenalis and Dientamoeba fragilis among the older children. Conclusions Stool pathogens proved common among all the children regardless of them having diarrhoea or not. |
format |
Article in Journal/Newspaper |
author |
Sointu Mero Suvi Timonen Tinja Lääveri Sandra Løfberg Juha Kirveskari Johan Ursing Lars Rombo Poul-Erik Kofoed Anu Kantele |
author_facet |
Sointu Mero Suvi Timonen Tinja Lääveri Sandra Løfberg Juha Kirveskari Johan Ursing Lars Rombo Poul-Erik Kofoed Anu Kantele |
author_sort |
Sointu Mero |
title |
Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau. |
title_short |
Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau. |
title_full |
Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau. |
title_fullStr |
Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau. |
title_full_unstemmed |
Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau. |
title_sort |
prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in guinea-bissau. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009709 https://doaj.org/article/c3a52d288cb4492b99babd02e8a60a6c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009709 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009709 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009709 https://doaj.org/article/c3a52d288cb4492b99babd02e8a60a6c |
op_doi |
https://doi.org/10.1371/journal.pntd.0009709 |
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PLOS Neglected Tropical Diseases |
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15 |
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9 |
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e0009709 |
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