Wolbachia Reduces the Transmission Potential of Dengue-Infected Aedes aegypti.
BACKGROUND:Dengue viruses (DENV) are the causative agents of dengue, the world's most prevalent arthropod-borne disease with around 40% of the world's population at risk of infection annually. Wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol stra...
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ftdoajarticles:oai:doaj.org/article:c37fd8227d914f02aeb4a7b47ebcb6d0 2023-05-15T15:15:54+02:00 Wolbachia Reduces the Transmission Potential of Dengue-Infected Aedes aegypti. Yixin H Ye Alison M Carrasco Francesca D Frentiu Stephen F Chenoweth Nigel W Beebe Andrew F van den Hurk Cameron P Simmons Scott L O'Neill Elizabeth A McGraw 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003894 https://doaj.org/article/c37fd8227d914f02aeb4a7b47ebcb6d0 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4482661?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003894 https://doaj.org/article/c37fd8227d914f02aeb4a7b47ebcb6d0 PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003894 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003894 2022-12-31T08:43:03Z BACKGROUND:Dengue viruses (DENV) are the causative agents of dengue, the world's most prevalent arthropod-borne disease with around 40% of the world's population at risk of infection annually. Wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits replication of the virus in the mosquito. The Wolbachia strain wMel, which has been introduced into the mosquito vector, Aedes aegypti, has been shown to invade and spread to near fixation in field releases. Standard measures of Wolbachia's efficacy for blocking virus replication focus on the detection and quantification of virus in mosquito tissues. Examining the saliva provides a more accurate measure of transmission potential and can reveal the extrinsic incubation period (EIP), that is, the time it takes virus to arrive in the saliva following the consumption of DENV viremic blood. EIP is a key determinant of a mosquito's ability to transmit DENVs, as the earlier the virus appears in the saliva the more opportunities the mosquito will have to infect humans on subsequent bites. METHODOLOGY/PRINCIPAL FINDINGS:We used a non-destructive assay to repeatedly quantify DENV in saliva from wMel-infected and Wolbachia-free wild-type control mosquitoes following the consumption of a DENV-infected blood meal. We show that wMel lengthens the EIP, reduces the frequency at which the virus is expectorated and decreases the dengue copy number in mosquito saliva as compared to wild-type mosquitoes. These observations can at least be partially explained by an overall reduction in saliva produced by wMel mosquitoes. More generally, we found that the concentration of DENV in a blood meal is a determinant of the length of EIP, saliva virus titer and mosquito survival. CONCLUSIONS/SIGNIFICANCE:The saliva-based traits reported here offer more disease-relevant measures of Wolbachia's effects on the vector and the virus. The lengthening of EIP highlights another means, in addition to the reduction of infection ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 6 e0003894 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yixin H Ye Alison M Carrasco Francesca D Frentiu Stephen F Chenoweth Nigel W Beebe Andrew F van den Hurk Cameron P Simmons Scott L O'Neill Elizabeth A McGraw Wolbachia Reduces the Transmission Potential of Dengue-Infected Aedes aegypti. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Dengue viruses (DENV) are the causative agents of dengue, the world's most prevalent arthropod-borne disease with around 40% of the world's population at risk of infection annually. Wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits replication of the virus in the mosquito. The Wolbachia strain wMel, which has been introduced into the mosquito vector, Aedes aegypti, has been shown to invade and spread to near fixation in field releases. Standard measures of Wolbachia's efficacy for blocking virus replication focus on the detection and quantification of virus in mosquito tissues. Examining the saliva provides a more accurate measure of transmission potential and can reveal the extrinsic incubation period (EIP), that is, the time it takes virus to arrive in the saliva following the consumption of DENV viremic blood. EIP is a key determinant of a mosquito's ability to transmit DENVs, as the earlier the virus appears in the saliva the more opportunities the mosquito will have to infect humans on subsequent bites. METHODOLOGY/PRINCIPAL FINDINGS:We used a non-destructive assay to repeatedly quantify DENV in saliva from wMel-infected and Wolbachia-free wild-type control mosquitoes following the consumption of a DENV-infected blood meal. We show that wMel lengthens the EIP, reduces the frequency at which the virus is expectorated and decreases the dengue copy number in mosquito saliva as compared to wild-type mosquitoes. These observations can at least be partially explained by an overall reduction in saliva produced by wMel mosquitoes. More generally, we found that the concentration of DENV in a blood meal is a determinant of the length of EIP, saliva virus titer and mosquito survival. CONCLUSIONS/SIGNIFICANCE:The saliva-based traits reported here offer more disease-relevant measures of Wolbachia's effects on the vector and the virus. The lengthening of EIP highlights another means, in addition to the reduction of infection ... |
format |
Article in Journal/Newspaper |
author |
Yixin H Ye Alison M Carrasco Francesca D Frentiu Stephen F Chenoweth Nigel W Beebe Andrew F van den Hurk Cameron P Simmons Scott L O'Neill Elizabeth A McGraw |
author_facet |
Yixin H Ye Alison M Carrasco Francesca D Frentiu Stephen F Chenoweth Nigel W Beebe Andrew F van den Hurk Cameron P Simmons Scott L O'Neill Elizabeth A McGraw |
author_sort |
Yixin H Ye |
title |
Wolbachia Reduces the Transmission Potential of Dengue-Infected Aedes aegypti. |
title_short |
Wolbachia Reduces the Transmission Potential of Dengue-Infected Aedes aegypti. |
title_full |
Wolbachia Reduces the Transmission Potential of Dengue-Infected Aedes aegypti. |
title_fullStr |
Wolbachia Reduces the Transmission Potential of Dengue-Infected Aedes aegypti. |
title_full_unstemmed |
Wolbachia Reduces the Transmission Potential of Dengue-Infected Aedes aegypti. |
title_sort |
wolbachia reduces the transmission potential of dengue-infected aedes aegypti. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003894 https://doaj.org/article/c37fd8227d914f02aeb4a7b47ebcb6d0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003894 (2015) |
op_relation |
http://europepmc.org/articles/PMC4482661?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003894 https://doaj.org/article/c37fd8227d914f02aeb4a7b47ebcb6d0 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003894 |
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PLOS Neglected Tropical Diseases |
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9 |
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6 |
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e0003894 |
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