Oxacillin disk diffusion testing for the prediction of penicillin resistance in Streptococcus pneumoniae
ABSTRACT Objective To 1) describe the correlation between the zones of inhibition in 1-µg oxacillin disk diffusion (ODD) tests and penicillin and ceftriaxone minimum inhibitory concentrations (MICs) of meningeal and non-meningeal strains of Streptococcus pneumoniae and 2) evaluate the usefulness of...
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ftdoajarticles:oai:doaj.org/article:c37a5d03b5cc4e2682253ecbce13eacd 2023-05-15T15:13:29+02:00 Oxacillin disk diffusion testing for the prediction of penicillin resistance in Streptococcus pneumoniae Gertrudis Horna María L. Molero Liliana Benites Sigri Roman Luz Carbajal Erik Mercado María E. Castillo Rito Zerpa Eduardo Chaparro Roger Hernandez Wilda Silva Francisco Campos Andy Saenz Isabel Reyes Alex Villalobos Theresa J. Ochoa https://doaj.org/article/c37a5d03b5cc4e2682253ecbce13eacd EN ES PT eng spa por Pan American Health Organization http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892016000700057&lng=en&tlng=en https://doaj.org/toc/1680-5348 1680-5348 https://doaj.org/article/c37a5d03b5cc4e2682253ecbce13eacd Revista Panamericana de Salud Pública, Vol 40, Iss 1, Pp 57-63 Streptococcus pneumoniae oxacillin Peru Latin America Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article ftdoajarticles 2022-12-31T13:23:00Z ABSTRACT Objective To 1) describe the correlation between the zones of inhibition in 1-µg oxacillin disk diffusion (ODD) tests and penicillin and ceftriaxone minimum inhibitory concentrations (MICs) of meningeal and non-meningeal strains of Streptococcus pneumoniae and 2) evaluate the usefulness of the ODD test as a predictor of susceptibility to penicillin in S. pneumoniae and as a quick and cost-effective method easily implemented in a routine clinical laboratory setting. Methods S. pneumoniae isolates from healthy nasopharyngeal carriers less than 2 years old, obtained in a multicentric cross-sectional study conducted in various Peruvian hospitals and health centers from 2007 to 2009, were analyzed. Using Clinical and Laboratory Standards Institute (CLSI) breakpoints, the correlation between the zones of inhibition of the ODD test and the MICs of penicillin and ceftriaxone was determined. Results Of the 571 S. pneumoniae isolates, 314 (55%) showed resistance to penicillin (MIC ≥ 0.12 µg/mL) and 124 (21.7%) showed resistance to ceftriaxone (MIC ≥ 1 µg/mL). Comparison of the ODD test zones of inhibition and the penicillin MICs, using the CLSI meningeal breakpoints, showed good correlation (Cohen’s kappa coefficient = 0.8239). Conclusions There was good correlation between ODD zones of inhibition and penicillin meningeal breakpoints but weak correlation between the ODD results and non-meningeal breakpoints for both penicillin and ceftriaxone. Therefore, the ODD test appears to be a useful tool for predicting penicillin resistance in cases of meningeal strains of S. pneumoniae, particularly in low- and middle- income countries, where MIC determination is not routinely available. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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English Spanish Portuguese |
topic |
Streptococcus pneumoniae oxacillin Peru Latin America Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Streptococcus pneumoniae oxacillin Peru Latin America Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gertrudis Horna María L. Molero Liliana Benites Sigri Roman Luz Carbajal Erik Mercado María E. Castillo Rito Zerpa Eduardo Chaparro Roger Hernandez Wilda Silva Francisco Campos Andy Saenz Isabel Reyes Alex Villalobos Theresa J. Ochoa Oxacillin disk diffusion testing for the prediction of penicillin resistance in Streptococcus pneumoniae |
topic_facet |
Streptococcus pneumoniae oxacillin Peru Latin America Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
ABSTRACT Objective To 1) describe the correlation between the zones of inhibition in 1-µg oxacillin disk diffusion (ODD) tests and penicillin and ceftriaxone minimum inhibitory concentrations (MICs) of meningeal and non-meningeal strains of Streptococcus pneumoniae and 2) evaluate the usefulness of the ODD test as a predictor of susceptibility to penicillin in S. pneumoniae and as a quick and cost-effective method easily implemented in a routine clinical laboratory setting. Methods S. pneumoniae isolates from healthy nasopharyngeal carriers less than 2 years old, obtained in a multicentric cross-sectional study conducted in various Peruvian hospitals and health centers from 2007 to 2009, were analyzed. Using Clinical and Laboratory Standards Institute (CLSI) breakpoints, the correlation between the zones of inhibition of the ODD test and the MICs of penicillin and ceftriaxone was determined. Results Of the 571 S. pneumoniae isolates, 314 (55%) showed resistance to penicillin (MIC ≥ 0.12 µg/mL) and 124 (21.7%) showed resistance to ceftriaxone (MIC ≥ 1 µg/mL). Comparison of the ODD test zones of inhibition and the penicillin MICs, using the CLSI meningeal breakpoints, showed good correlation (Cohen’s kappa coefficient = 0.8239). Conclusions There was good correlation between ODD zones of inhibition and penicillin meningeal breakpoints but weak correlation between the ODD results and non-meningeal breakpoints for both penicillin and ceftriaxone. Therefore, the ODD test appears to be a useful tool for predicting penicillin resistance in cases of meningeal strains of S. pneumoniae, particularly in low- and middle- income countries, where MIC determination is not routinely available. |
format |
Article in Journal/Newspaper |
author |
Gertrudis Horna María L. Molero Liliana Benites Sigri Roman Luz Carbajal Erik Mercado María E. Castillo Rito Zerpa Eduardo Chaparro Roger Hernandez Wilda Silva Francisco Campos Andy Saenz Isabel Reyes Alex Villalobos Theresa J. Ochoa |
author_facet |
Gertrudis Horna María L. Molero Liliana Benites Sigri Roman Luz Carbajal Erik Mercado María E. Castillo Rito Zerpa Eduardo Chaparro Roger Hernandez Wilda Silva Francisco Campos Andy Saenz Isabel Reyes Alex Villalobos Theresa J. Ochoa |
author_sort |
Gertrudis Horna |
title |
Oxacillin disk diffusion testing for the prediction of penicillin resistance in Streptococcus pneumoniae |
title_short |
Oxacillin disk diffusion testing for the prediction of penicillin resistance in Streptococcus pneumoniae |
title_full |
Oxacillin disk diffusion testing for the prediction of penicillin resistance in Streptococcus pneumoniae |
title_fullStr |
Oxacillin disk diffusion testing for the prediction of penicillin resistance in Streptococcus pneumoniae |
title_full_unstemmed |
Oxacillin disk diffusion testing for the prediction of penicillin resistance in Streptococcus pneumoniae |
title_sort |
oxacillin disk diffusion testing for the prediction of penicillin resistance in streptococcus pneumoniae |
publisher |
Pan American Health Organization |
url |
https://doaj.org/article/c37a5d03b5cc4e2682253ecbce13eacd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista Panamericana de Salud Pública, Vol 40, Iss 1, Pp 57-63 |
op_relation |
http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892016000700057&lng=en&tlng=en https://doaj.org/toc/1680-5348 1680-5348 https://doaj.org/article/c37a5d03b5cc4e2682253ecbce13eacd |
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1766344043547066368 |