Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway
Oyster peptide (OP) has exhibited useful biological activities and can be used in multi-functional foods. OP has been reported to play a significant role in intestinal protection, but its specific mechanism is still not completely understood. The aim of this study was to analyze the potential effect...
Published in: | Frontiers in Nutrition |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fnut.2022.888960 https://doaj.org/article/c31ff0d297b34e7cb7bac35d6bbaacd2 |
id |
ftdoajarticles:oai:doaj.org/article:c31ff0d297b34e7cb7bac35d6bbaacd2 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:c31ff0d297b34e7cb7bac35d6bbaacd2 2023-05-15T15:58:53+02:00 Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway Hui Chen Huizhen Zheng Tiejun Li Qihong Jiang Shulai Liu Xuxia Zhou Yuting Ding Xingwei Xiang 2022-05-01T00:00:00Z https://doi.org/10.3389/fnut.2022.888960 https://doaj.org/article/c31ff0d297b34e7cb7bac35d6bbaacd2 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fnut.2022.888960/full https://doaj.org/toc/2296-861X 2296-861X doi:10.3389/fnut.2022.888960 https://doaj.org/article/c31ff0d297b34e7cb7bac35d6bbaacd2 Frontiers in Nutrition, Vol 9 (2022) oyster peptides cyclophosphamide oxidative stress intestinal mucosa Nrf2-Keap1 antioxidant pathway Nutrition. Foods and food supply TX341-641 article 2022 ftdoajarticles https://doi.org/10.3389/fnut.2022.888960 2022-12-30T21:28:31Z Oyster peptide (OP) has exhibited useful biological activities and can be used in multi-functional foods. OP has been reported to play a significant role in intestinal protection, but its specific mechanism is still not completely understood. The aim of this study was to analyze the potential effect of OP on oxidative damage of mice intestine induced by cyclophosphamide (Cy). The experimental results revealed that intragastric administration of OP significantly increased average bodyweight, improved ileum tissue morphology and villus structure, as well as increased the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in oxidized mice serum and liver. The content of malondialdehyde (MDA) in the mice serum and liver homogenate was found to be markedly decreased. Moreover, OP significantly increased the relative mRNA expression levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), quinone oxidoreductase (NQO1) and heme oxidase-1 (HO-1) in ileum. Western-blot results indicated that prior administration of OP significantly up-regulated the Nrf2 production in ileum, and substantially decreased then Keap1 gene expression. In conclusion, intake of OP was found to markedly improve intestinal oxidative stress in vivo, and this effect was primarily mediated through the simulation of antioxidant Nrf2-Keap1 signaling pathway. This study is beneficial to the application of peptide nutrients in the prevention or mitigation of intestinal oxidative damage. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Frontiers in Nutrition 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
oyster peptides cyclophosphamide oxidative stress intestinal mucosa Nrf2-Keap1 antioxidant pathway Nutrition. Foods and food supply TX341-641 |
spellingShingle |
oyster peptides cyclophosphamide oxidative stress intestinal mucosa Nrf2-Keap1 antioxidant pathway Nutrition. Foods and food supply TX341-641 Hui Chen Huizhen Zheng Tiejun Li Qihong Jiang Shulai Liu Xuxia Zhou Yuting Ding Xingwei Xiang Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway |
topic_facet |
oyster peptides cyclophosphamide oxidative stress intestinal mucosa Nrf2-Keap1 antioxidant pathway Nutrition. Foods and food supply TX341-641 |
description |
Oyster peptide (OP) has exhibited useful biological activities and can be used in multi-functional foods. OP has been reported to play a significant role in intestinal protection, but its specific mechanism is still not completely understood. The aim of this study was to analyze the potential effect of OP on oxidative damage of mice intestine induced by cyclophosphamide (Cy). The experimental results revealed that intragastric administration of OP significantly increased average bodyweight, improved ileum tissue morphology and villus structure, as well as increased the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in oxidized mice serum and liver. The content of malondialdehyde (MDA) in the mice serum and liver homogenate was found to be markedly decreased. Moreover, OP significantly increased the relative mRNA expression levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), quinone oxidoreductase (NQO1) and heme oxidase-1 (HO-1) in ileum. Western-blot results indicated that prior administration of OP significantly up-regulated the Nrf2 production in ileum, and substantially decreased then Keap1 gene expression. In conclusion, intake of OP was found to markedly improve intestinal oxidative stress in vivo, and this effect was primarily mediated through the simulation of antioxidant Nrf2-Keap1 signaling pathway. This study is beneficial to the application of peptide nutrients in the prevention or mitigation of intestinal oxidative damage. |
format |
Article in Journal/Newspaper |
author |
Hui Chen Huizhen Zheng Tiejun Li Qihong Jiang Shulai Liu Xuxia Zhou Yuting Ding Xingwei Xiang |
author_facet |
Hui Chen Huizhen Zheng Tiejun Li Qihong Jiang Shulai Liu Xuxia Zhou Yuting Ding Xingwei Xiang |
author_sort |
Hui Chen |
title |
Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway |
title_short |
Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway |
title_full |
Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway |
title_fullStr |
Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway |
title_full_unstemmed |
Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway |
title_sort |
protective effect of oyster peptides derived from crassostrea gigas on intestinal oxidative damage induced by cyclophosphamide in mice mediated through nrf2-keap1 signaling pathway |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fnut.2022.888960 https://doaj.org/article/c31ff0d297b34e7cb7bac35d6bbaacd2 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Nutrition, Vol 9 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fnut.2022.888960/full https://doaj.org/toc/2296-861X 2296-861X doi:10.3389/fnut.2022.888960 https://doaj.org/article/c31ff0d297b34e7cb7bac35d6bbaacd2 |
op_doi |
https://doi.org/10.3389/fnut.2022.888960 |
container_title |
Frontiers in Nutrition |
container_volume |
9 |
_version_ |
1766394648423563264 |