Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6
Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clus...
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2021
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ftdoajarticles:oai:doaj.org/article:c311799de67a47c5a2605ec2f0033cda 2023-05-15T14:54:20+02:00 Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 Tae-Rim Choi Ye-Lim Park Hun-Suk Song Sun Mi Lee Sol Lee Park Hye Soo Lee Hyun-Joong Kim Shashi Kant Bhatia Ranjit Gurav Kwon-Young Choi Yoo Kyung Lee Yung-Hun Yang 2021-04-01T00:00:00Z https://doi.org/10.3390/polym13091398 https://doaj.org/article/c311799de67a47c5a2605ec2f0033cda EN eng MDPI AG https://www.mdpi.com/2073-4360/13/9/1398 https://doaj.org/toc/2073-4360 doi:10.3390/polym13091398 2073-4360 https://doaj.org/article/c311799de67a47c5a2605ec2f0033cda Polymers, Vol 13, Iss 1398, p 1398 (2021) Pseudomonas strain polyhydroxyalkanoate fructose syrup fermentation Organic chemistry QD241-441 article 2021 ftdoajarticles https://doi.org/10.3390/polym13091398 2022-12-31T10:37:06Z Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes ( phaC ). Pseudomonas sp. B14-6 produced poly(6% 3-hydroxybutyrate- co -94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl- co -mcl PHA determined a glass transition temperature (T g ) of 15.3 °C, melting temperature of 112.8 °C, crystallization temperature of 86.8 °C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 × 10 4 , weight average molecular weight of 9.1 × 10 4 , and polydispersity index value of 2.5. Overall, the novel Pseudomonas sp. B14-6 produced a polymer with high medium-chain-length content, low T g , and low crystallinity, indicating its potential use in medical applications. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Polymers 13 9 1398 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pseudomonas strain polyhydroxyalkanoate fructose syrup fermentation Organic chemistry QD241-441 |
spellingShingle |
Pseudomonas strain polyhydroxyalkanoate fructose syrup fermentation Organic chemistry QD241-441 Tae-Rim Choi Ye-Lim Park Hun-Suk Song Sun Mi Lee Sol Lee Park Hye Soo Lee Hyun-Joong Kim Shashi Kant Bhatia Ranjit Gurav Kwon-Young Choi Yoo Kyung Lee Yung-Hun Yang Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
topic_facet |
Pseudomonas strain polyhydroxyalkanoate fructose syrup fermentation Organic chemistry QD241-441 |
description |
Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes ( phaC ). Pseudomonas sp. B14-6 produced poly(6% 3-hydroxybutyrate- co -94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl- co -mcl PHA determined a glass transition temperature (T g ) of 15.3 °C, melting temperature of 112.8 °C, crystallization temperature of 86.8 °C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 × 10 4 , weight average molecular weight of 9.1 × 10 4 , and polydispersity index value of 2.5. Overall, the novel Pseudomonas sp. B14-6 produced a polymer with high medium-chain-length content, low T g , and low crystallinity, indicating its potential use in medical applications. |
format |
Article in Journal/Newspaper |
author |
Tae-Rim Choi Ye-Lim Park Hun-Suk Song Sun Mi Lee Sol Lee Park Hye Soo Lee Hyun-Joong Kim Shashi Kant Bhatia Ranjit Gurav Kwon-Young Choi Yoo Kyung Lee Yung-Hun Yang |
author_facet |
Tae-Rim Choi Ye-Lim Park Hun-Suk Song Sun Mi Lee Sol Lee Park Hye Soo Lee Hyun-Joong Kim Shashi Kant Bhatia Ranjit Gurav Kwon-Young Choi Yoo Kyung Lee Yung-Hun Yang |
author_sort |
Tae-Rim Choi |
title |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_short |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_full |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_fullStr |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_full_unstemmed |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_sort |
fructose-based production of short-chain-length and medium-chain-length polyhydroxyalkanoate copolymer by arctic pseudomonas sp. b14-6 |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/polym13091398 https://doaj.org/article/c311799de67a47c5a2605ec2f0033cda |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Polymers, Vol 13, Iss 1398, p 1398 (2021) |
op_relation |
https://www.mdpi.com/2073-4360/13/9/1398 https://doaj.org/toc/2073-4360 doi:10.3390/polym13091398 2073-4360 https://doaj.org/article/c311799de67a47c5a2605ec2f0033cda |
op_doi |
https://doi.org/10.3390/polym13091398 |
container_title |
Polymers |
container_volume |
13 |
container_issue |
9 |
container_start_page |
1398 |
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1766326057988784128 |