Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages
Objective: To investigate antioxidant and anti-inflammatory effects of Tagetes minuta (T. minuta) essential oil. Methods: In the present study T. minuta essential oil was obtained from leaves of T. minuta via hydro-distillation and then was analyzed by gas chromatography-mass spectrometry. The anti-...
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ftdoajarticles:oai:doaj.org/article:c304dff538fd41ee8ca26a2ad0599e80 2023-05-15T15:10:32+02:00 Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages Parastoo Karimian Gholamreza Kavoosi Zahra Amirghofran 2014-03-01T00:00:00Z https://doi.org/10.1016/S2221-1691(14)60235-5 https://doaj.org/article/c304dff538fd41ee8ca26a2ad0599e80 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115301969 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(14)60235-5 https://doaj.org/article/c304dff538fd41ee8ca26a2ad0599e80 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 3, Pp 219-227 (2014) Tagetes minuta Essential oil Macrophages Anti-inflammatory Antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.1016/S2221-1691(14)60235-5 2022-12-31T00:39:38Z Objective: To investigate antioxidant and anti-inflammatory effects of Tagetes minuta (T. minuta) essential oil. Methods: In the present study T. minuta essential oil was obtained from leaves of T. minuta via hydro-distillation and then was analyzed by gas chromatography-mass spectrometry. The anti-oxidant capacity of T. minuta essential oil was examined by measuring reactive oxygen, reactive nitrogen species and hydrogen peroxide scavenging. The anti-inflammatory activity of T. minuta essential oil was determined through measuring NADH oxidase, inducible nitric oxide synthase and TNF-α mRNA expression in lipopolysacharide-stimulated murine macrophages using real-time PCR. Results: Gas chromatography-mass spectrometry analysis indicated that the main components in the T. minuta essential oil were dihydrotagetone (33.86%), E-ocimene (19.92%), tagetone (16.15%), cis-β-ocimene (7.94%), Z-ocimene (5.27%), limonene (3.1%) and epoxyocimene (2.03%). The T. minuta essential oil had the ability to scavenge all reactive oxygen/reactive nitrogen species radicals with IC50 12-15 μg/mL, which indicated a potent radical scavenging activity. In addition, T. minuta essential oil significantly reduced NADH oxidase, inducible nitric oxide synthaseand TNF-α mRNA expression in the cells at concentrations of 50 μg/mL, indicating a capacity of this product to potentially modulate/diminish immune responses. Conclusions: T. minuta essential oil has radical scavenging and anti-inflammatory activities and could potentially be used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and stress associated with some inflammatory conditions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 3 219 227 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Tagetes minuta Essential oil Macrophages Anti-inflammatory Antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Tagetes minuta Essential oil Macrophages Anti-inflammatory Antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Parastoo Karimian Gholamreza Kavoosi Zahra Amirghofran Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages |
topic_facet |
Tagetes minuta Essential oil Macrophages Anti-inflammatory Antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate antioxidant and anti-inflammatory effects of Tagetes minuta (T. minuta) essential oil. Methods: In the present study T. minuta essential oil was obtained from leaves of T. minuta via hydro-distillation and then was analyzed by gas chromatography-mass spectrometry. The anti-oxidant capacity of T. minuta essential oil was examined by measuring reactive oxygen, reactive nitrogen species and hydrogen peroxide scavenging. The anti-inflammatory activity of T. minuta essential oil was determined through measuring NADH oxidase, inducible nitric oxide synthase and TNF-α mRNA expression in lipopolysacharide-stimulated murine macrophages using real-time PCR. Results: Gas chromatography-mass spectrometry analysis indicated that the main components in the T. minuta essential oil were dihydrotagetone (33.86%), E-ocimene (19.92%), tagetone (16.15%), cis-β-ocimene (7.94%), Z-ocimene (5.27%), limonene (3.1%) and epoxyocimene (2.03%). The T. minuta essential oil had the ability to scavenge all reactive oxygen/reactive nitrogen species radicals with IC50 12-15 μg/mL, which indicated a potent radical scavenging activity. In addition, T. minuta essential oil significantly reduced NADH oxidase, inducible nitric oxide synthaseand TNF-α mRNA expression in the cells at concentrations of 50 μg/mL, indicating a capacity of this product to potentially modulate/diminish immune responses. Conclusions: T. minuta essential oil has radical scavenging and anti-inflammatory activities and could potentially be used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and stress associated with some inflammatory conditions. |
format |
Article in Journal/Newspaper |
author |
Parastoo Karimian Gholamreza Kavoosi Zahra Amirghofran |
author_facet |
Parastoo Karimian Gholamreza Kavoosi Zahra Amirghofran |
author_sort |
Parastoo Karimian |
title |
Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages |
title_short |
Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages |
title_full |
Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages |
title_fullStr |
Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages |
title_full_unstemmed |
Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages |
title_sort |
anti–oxidative and anti–inflammatory effects of tagetes minuta essential oil in activated macrophages |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.1016/S2221-1691(14)60235-5 https://doaj.org/article/c304dff538fd41ee8ca26a2ad0599e80 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 3, Pp 219-227 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115301969 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(14)60235-5 https://doaj.org/article/c304dff538fd41ee8ca26a2ad0599e80 |
op_doi |
https://doi.org/10.1016/S2221-1691(14)60235-5 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
3 |
container_start_page |
219 |
op_container_end_page |
227 |
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1766341550033338368 |