Antioxidant potential of Rumex vesicarius L.: in vitro approach

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Tajdar Husain Khan, Majid Ahmad Ganaie, Nasir Ali Siddiqui, Aftab Alam, Mohd Nazam Ansari
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014
• Subjects: Rumex vesicarius L; DNA sugar damage; DPPH; antioxidant; HPTLC; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.12980/APJTB.4.2014C1168; https://doaj.org/article/c2f5476f0d6c4e0dac8bb27cc444e19f

Objective: To assess in-vitro antioxidant activity of different fraction and perform high performance thin layer chromatography fingerprint analysis of most active fraction of Rumex vesicarius L. (R. vesicarius). Methods: In the present study, acetone, ethyl acetate, n-butanol, and methanol extracts of R. vesicarius were evaluated for radical scavenging activity by studying the inhibition of the level of lipid peroxidation induced by Fe(++)/ascorbate, DNA sugar damage, scavenging of hydrogen peroxide, diphenylphosphine DPPH radical scavenging activity, total phenolic content, total flavonoids content and total proanthocyanidin. High performance thin layer chromatography finger print profiling of R. vesicarius L. was also done. Results: Lipid peroxidation induced by the iron/ascorbate system, hydrogen peroxide, diphenylphosphine and DNA sugar damage were inhibited by the addition of different extract of R. vesicarius. Among them, methanolic extract showed maximum efficacy. The methanolic extract showed the highest total phenolic, total flavonoids and total proanthocyanidin contents. Conclusions: The results suggest that the extracts can be a vital source of phytochemical antioxidants.