Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus )
Abstract Background Two outbreaks of the disease contagious ecthyma were reported in 1999 and 2000 in Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ). Contagious ecthyma is an epidermal disease of sheep and goats worldwide, which is caused by the zoonotic parapoxvirus orf virus....
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ftdoajarticles:oai:doaj.org/article:c2ee6a34e590484eaa34fd005b30e831 2023-05-15T18:04:16+02:00 Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ) Klein Joern Tryland Morten 2005-09-01T00:00:00Z https://doi.org/10.1186/1743-422X-2-79 https://doaj.org/article/c2ee6a34e590484eaa34fd005b30e831 EN eng BMC http://www.virologyj.com/content/2/1/79 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-2-79 1743-422X https://doaj.org/article/c2ee6a34e590484eaa34fd005b30e831 Virology Journal, Vol 2, Iss 1, p 79 (2005) Infectious and parasitic diseases RC109-216 article 2005 ftdoajarticles https://doi.org/10.1186/1743-422X-2-79 2022-12-31T12:31:37Z Abstract Background Two outbreaks of the disease contagious ecthyma were reported in 1999 and 2000 in Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ). Contagious ecthyma is an epidermal disease of sheep and goats worldwide, which is caused by the zoonotic parapoxvirus orf virus. Characterisation of clinical samples from the two outbreaks in semi-domesticated reindeer in Norway by electron microscopy and PCR (B2L) revealed typical parapoxvirus particles and partial gene sequences corresponding to parapoxvirus, respectively. If contagious ecthyma in reindeer is caused by orf virus, the virus may be transferred from sheep and goats, via people, equipment and common use of pastures and corrals, to reindeer. Another possibility is that contagious ecthyma in reindeer is caused by a hitherto unclassified member of the parapoxvirus genus that circulates among reindeer herds and remains endemic in Norway. Results Genomic comparisons of one standard orf strain (orf NZ2) and the reindeer isolates, employing restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) analysis, demonstrated high similarity between the reindeer viruses and known orf virus strains. Partial DNA sequences of two different viral genes were determined for the different isolates and compared with corresponding parapoxvirus genebank sequences. The comparison/alignment and construction of phylogenetic trees also point to an affiliation of the reindeer viruses to the species orf virus. Conclusion The results of this work imply that the parapoxvirus causing contagious ecthyma in Norwegian semi-domesticated reindeer belongs to the species orf virus and that the orf virus crosses the host species barrier from sheep and goat to semi-domesticated reindeer. Article in Journal/Newspaper Rangifer tarandus Directory of Open Access Journals: DOAJ Articles Norway Virology Journal 2 1 79 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Infectious and parasitic diseases RC109-216 |
spellingShingle |
Infectious and parasitic diseases RC109-216 Klein Joern Tryland Morten Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ) |
topic_facet |
Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Two outbreaks of the disease contagious ecthyma were reported in 1999 and 2000 in Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ). Contagious ecthyma is an epidermal disease of sheep and goats worldwide, which is caused by the zoonotic parapoxvirus orf virus. Characterisation of clinical samples from the two outbreaks in semi-domesticated reindeer in Norway by electron microscopy and PCR (B2L) revealed typical parapoxvirus particles and partial gene sequences corresponding to parapoxvirus, respectively. If contagious ecthyma in reindeer is caused by orf virus, the virus may be transferred from sheep and goats, via people, equipment and common use of pastures and corrals, to reindeer. Another possibility is that contagious ecthyma in reindeer is caused by a hitherto unclassified member of the parapoxvirus genus that circulates among reindeer herds and remains endemic in Norway. Results Genomic comparisons of one standard orf strain (orf NZ2) and the reindeer isolates, employing restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) analysis, demonstrated high similarity between the reindeer viruses and known orf virus strains. Partial DNA sequences of two different viral genes were determined for the different isolates and compared with corresponding parapoxvirus genebank sequences. The comparison/alignment and construction of phylogenetic trees also point to an affiliation of the reindeer viruses to the species orf virus. Conclusion The results of this work imply that the parapoxvirus causing contagious ecthyma in Norwegian semi-domesticated reindeer belongs to the species orf virus and that the orf virus crosses the host species barrier from sheep and goat to semi-domesticated reindeer. |
format |
Article in Journal/Newspaper |
author |
Klein Joern Tryland Morten |
author_facet |
Klein Joern Tryland Morten |
author_sort |
Klein Joern |
title |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ) |
title_short |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ) |
title_full |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ) |
title_fullStr |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ) |
title_full_unstemmed |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ) |
title_sort |
characterisation of parapoxviruses isolated from norwegian semi-domesticated reindeer ( rangifer tarandus tarandus ) |
publisher |
BMC |
publishDate |
2005 |
url |
https://doi.org/10.1186/1743-422X-2-79 https://doaj.org/article/c2ee6a34e590484eaa34fd005b30e831 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_source |
Virology Journal, Vol 2, Iss 1, p 79 (2005) |
op_relation |
http://www.virologyj.com/content/2/1/79 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-2-79 1743-422X https://doaj.org/article/c2ee6a34e590484eaa34fd005b30e831 |
op_doi |
https://doi.org/10.1186/1743-422X-2-79 |
container_title |
Virology Journal |
container_volume |
2 |
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1 |
container_start_page |
79 |
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1766175595986681856 |