eDNA metabarcoding reveals riverine fish community structure and climate associations in northeastern Canada
Abstract Climate change is a critical threat to northern freshwater ecosystems, yet many remote areas are data deficient in terms of biodiversity information. Generating community composition data through collection of environmental DNA (eDNA) is less labor‐intensive than traditional sampling method...
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ftdoajarticles:oai:doaj.org/article:c2ddad5c8a25434383b351855f42d84e 2024-09-15T18:20:02+00:00 eDNA metabarcoding reveals riverine fish community structure and climate associations in northeastern Canada Samantha E. Crowley Paul Bentzen Tony Kess Steven J. Duffy Amber M. Messmer Beth Watson J. Brian Dempson Donald G. Keefe Robert C. Perry Benjamin Marquis Mehrdad Hajibabaei Nicole Fahner Lesley Berghuis Kerry Hobrecker Ian R. Bradbury 2024-01-01T00:00:00Z https://doi.org/10.1002/edn3.517 https://doaj.org/article/c2ddad5c8a25434383b351855f42d84e EN eng Wiley https://doi.org/10.1002/edn3.517 https://doaj.org/toc/2637-4943 2637-4943 doi:10.1002/edn3.517 https://doaj.org/article/c2ddad5c8a25434383b351855f42d84e Environmental DNA, Vol 6, Iss 1, Pp n/a-n/a (2024) climate community composition environmental DNA fish metabarcoding Environmental sciences GE1-350 Microbial ecology QR100-130 article 2024 ftdoajarticles https://doi.org/10.1002/edn3.517 2024-08-05T17:49:53Z Abstract Climate change is a critical threat to northern freshwater ecosystems, yet many remote areas are data deficient in terms of biodiversity information. Generating community composition data through collection of environmental DNA (eDNA) is less labor‐intensive than traditional sampling methods and is being increasingly used in areas that have been historically difficult to sample such as northern freshwater habitats. Here, we employed eDNA metabarcoding using three mitochondrial markers at 174 coastal river sites, sampled over three years (2019–2021) across a broad region in northeastern North America, Newfoundland and Labrador. We characterized current riverine fish community composition, compared it to traditional sampling records, and quantified the influence of climate on variation in fish community composition. The analysis detected 33 fish species across the region (1–13 per location), including three non‐native species, as well as several new possible range expansions. Variance partitioning with redundancy analysis indicated ~56% of the variation in community composition could be explained by spatial and climate factors (~21% and ~7%, respectively, with an additional ~28% shared). A temporal comparison across a subset of locations with both eDNA and historical records (1965–1985) revealed that more species were detected on average with eDNA sampling, and that sampling method explained a small portion of the variation (~4%) in comparison with space (~10%) and climate (~7%). Ultimately, this work is the most complete survey of freshwater and diadromous fishes present in Newfoundland and Labrador to date, highlights new detections of non‐native species including previously unknown diversity for the region, and provides future direction for the application of eDNA analysis in northern riverine habitats. Article in Journal/Newspaper Newfoundland Directory of Open Access Journals: DOAJ Articles Environmental DNA 6 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
climate community composition environmental DNA fish metabarcoding Environmental sciences GE1-350 Microbial ecology QR100-130 |
spellingShingle |
climate community composition environmental DNA fish metabarcoding Environmental sciences GE1-350 Microbial ecology QR100-130 Samantha E. Crowley Paul Bentzen Tony Kess Steven J. Duffy Amber M. Messmer Beth Watson J. Brian Dempson Donald G. Keefe Robert C. Perry Benjamin Marquis Mehrdad Hajibabaei Nicole Fahner Lesley Berghuis Kerry Hobrecker Ian R. Bradbury eDNA metabarcoding reveals riverine fish community structure and climate associations in northeastern Canada |
topic_facet |
climate community composition environmental DNA fish metabarcoding Environmental sciences GE1-350 Microbial ecology QR100-130 |
description |
Abstract Climate change is a critical threat to northern freshwater ecosystems, yet many remote areas are data deficient in terms of biodiversity information. Generating community composition data through collection of environmental DNA (eDNA) is less labor‐intensive than traditional sampling methods and is being increasingly used in areas that have been historically difficult to sample such as northern freshwater habitats. Here, we employed eDNA metabarcoding using three mitochondrial markers at 174 coastal river sites, sampled over three years (2019–2021) across a broad region in northeastern North America, Newfoundland and Labrador. We characterized current riverine fish community composition, compared it to traditional sampling records, and quantified the influence of climate on variation in fish community composition. The analysis detected 33 fish species across the region (1–13 per location), including three non‐native species, as well as several new possible range expansions. Variance partitioning with redundancy analysis indicated ~56% of the variation in community composition could be explained by spatial and climate factors (~21% and ~7%, respectively, with an additional ~28% shared). A temporal comparison across a subset of locations with both eDNA and historical records (1965–1985) revealed that more species were detected on average with eDNA sampling, and that sampling method explained a small portion of the variation (~4%) in comparison with space (~10%) and climate (~7%). Ultimately, this work is the most complete survey of freshwater and diadromous fishes present in Newfoundland and Labrador to date, highlights new detections of non‐native species including previously unknown diversity for the region, and provides future direction for the application of eDNA analysis in northern riverine habitats. |
format |
Article in Journal/Newspaper |
author |
Samantha E. Crowley Paul Bentzen Tony Kess Steven J. Duffy Amber M. Messmer Beth Watson J. Brian Dempson Donald G. Keefe Robert C. Perry Benjamin Marquis Mehrdad Hajibabaei Nicole Fahner Lesley Berghuis Kerry Hobrecker Ian R. Bradbury |
author_facet |
Samantha E. Crowley Paul Bentzen Tony Kess Steven J. Duffy Amber M. Messmer Beth Watson J. Brian Dempson Donald G. Keefe Robert C. Perry Benjamin Marquis Mehrdad Hajibabaei Nicole Fahner Lesley Berghuis Kerry Hobrecker Ian R. Bradbury |
author_sort |
Samantha E. Crowley |
title |
eDNA metabarcoding reveals riverine fish community structure and climate associations in northeastern Canada |
title_short |
eDNA metabarcoding reveals riverine fish community structure and climate associations in northeastern Canada |
title_full |
eDNA metabarcoding reveals riverine fish community structure and climate associations in northeastern Canada |
title_fullStr |
eDNA metabarcoding reveals riverine fish community structure and climate associations in northeastern Canada |
title_full_unstemmed |
eDNA metabarcoding reveals riverine fish community structure and climate associations in northeastern Canada |
title_sort |
edna metabarcoding reveals riverine fish community structure and climate associations in northeastern canada |
publisher |
Wiley |
publishDate |
2024 |
url |
https://doi.org/10.1002/edn3.517 https://doaj.org/article/c2ddad5c8a25434383b351855f42d84e |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Environmental DNA, Vol 6, Iss 1, Pp n/a-n/a (2024) |
op_relation |
https://doi.org/10.1002/edn3.517 https://doaj.org/toc/2637-4943 2637-4943 doi:10.1002/edn3.517 https://doaj.org/article/c2ddad5c8a25434383b351855f42d84e |
op_doi |
https://doi.org/10.1002/edn3.517 |
container_title |
Environmental DNA |
container_volume |
6 |
container_issue |
1 |
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1810458404964007936 |