Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms
Although sea anemones are well known for being rich sources of toxins, including cytolysins and neurotoxins, their venoms and toxins have been poorly studied. In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum a...
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ftdoajarticles:oai:doaj.org/article:c2d3040c705c45d6a1d05e736aa90cd6 2023-05-15T15:11:01+02:00 Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms M Ramezanpour K Burke da Silva BJ Sanderson 2012-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992012000200005 https://doaj.org/article/c2d3040c705c45d6a1d05e736aa90cd6 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200005 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000200005 1678-9199 https://doaj.org/article/c2d3040c705c45d6a1d05e736aa90cd6 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 2, Pp 157-163 (2012) sea anemone venoms cytolysin cancer cell line Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2012 ftdoajarticles https://doi.org/10.1590/S1678-91992012000200005 2022-12-31T00:18:12Z Although sea anemones are well known for being rich sources of toxins, including cytolysins and neurotoxins, their venoms and toxins have been poorly studied. In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum and Entacmaea quadricolor) were obtained by the milking technique, and the potential of these venoms to kill cancer cells was tested on three cell lines (A549 lung cancer, T47D breast cancer and A431 skin cancer). The total protein level in the crude extract was determined by the bicinchoninic acid (BCA) protein assay. The cytotoxicity on different cell lines was assayed using the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay which measures survival based on the detection of mitochondrial activity and by the crystal violet assay, which measures survival based on the ability of cells to remain adherent to microplates. The results indicate that the sea anemone venom is cytotoxic to human cancer cells. The A549 cell line was the most sensitive of the cell lines tested with a significant reduction in viability observed at 40 µg/mL. H. malu, C. adhaesivum and E. quadricolor had a significant inhibitory effect on A431 cells. Furthermore, H. malu and C. adhaesivum had a significant inhibitory effect on T47D cell line at 40 µg/mL. In conclusion, the sea anemone venoms tested have the potential to be developed as anticancer agents. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 18 2 157 163 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
sea anemone venoms cytolysin cancer cell line Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
sea anemone venoms cytolysin cancer cell line Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 M Ramezanpour K Burke da Silva BJ Sanderson Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms |
topic_facet |
sea anemone venoms cytolysin cancer cell line Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Although sea anemones are well known for being rich sources of toxins, including cytolysins and neurotoxins, their venoms and toxins have been poorly studied. In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum and Entacmaea quadricolor) were obtained by the milking technique, and the potential of these venoms to kill cancer cells was tested on three cell lines (A549 lung cancer, T47D breast cancer and A431 skin cancer). The total protein level in the crude extract was determined by the bicinchoninic acid (BCA) protein assay. The cytotoxicity on different cell lines was assayed using the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay which measures survival based on the detection of mitochondrial activity and by the crystal violet assay, which measures survival based on the ability of cells to remain adherent to microplates. The results indicate that the sea anemone venom is cytotoxic to human cancer cells. The A549 cell line was the most sensitive of the cell lines tested with a significant reduction in viability observed at 40 µg/mL. H. malu, C. adhaesivum and E. quadricolor had a significant inhibitory effect on A431 cells. Furthermore, H. malu and C. adhaesivum had a significant inhibitory effect on T47D cell line at 40 µg/mL. In conclusion, the sea anemone venoms tested have the potential to be developed as anticancer agents. |
format |
Article in Journal/Newspaper |
author |
M Ramezanpour K Burke da Silva BJ Sanderson |
author_facet |
M Ramezanpour K Burke da Silva BJ Sanderson |
author_sort |
M Ramezanpour |
title |
Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms |
title_short |
Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms |
title_full |
Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms |
title_fullStr |
Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms |
title_full_unstemmed |
Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms |
title_sort |
differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms |
publisher |
SciELO |
publishDate |
2012 |
url |
https://doi.org/10.1590/S1678-91992012000200005 https://doaj.org/article/c2d3040c705c45d6a1d05e736aa90cd6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 2, Pp 157-163 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200005 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000200005 1678-9199 https://doaj.org/article/c2d3040c705c45d6a1d05e736aa90cd6 |
op_doi |
https://doi.org/10.1590/S1678-91992012000200005 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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18 |
container_issue |
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157 |
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163 |
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