Molecular identification of genus Pipistrellus (Mammalia: Chiroptera) from Fata region, Pakistan

Abstract A total of 10 specimens were captured from selected sites of Bajaur Agency FATA, Pakistan using mist nets. The captured specimens were morphologically identified and various morphometric measurements were taken. The head and Body length (HB) of Pipistrellus coromondra and Pipistrellus kuhli...

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Bibliographic Details
Published in:Brazilian Journal of Biology
Main Authors: M. Idnan, A. Javid, M. Tayyab, A. Hussain, S. Mansoor, S. M. Bukhari, Irfan, M. Shahbaz, K. ur Rehman, S. Andleeb, S. M. Azam, W. Ali
Format: Article in Journal/Newspaper
Language:English
Published: Instituto Internacional de Ecologia 2021
Subjects:
Pipistrellus
Pipistrellus kuhlii lepidus
Pipistrellus kuhlii
Pipistrellus coromondra
Science
Q
Biology (General)
QH301-705.5
Zoology
QL1-991
Botany
QK1-989
Pipistrellus pipistrellus
Online Access:https://doi.org/10.1590/1519-6984.246322
https://doaj.org/article/c272346facba44fcb57082f2bcc016f7
Description
Summary:Abstract A total of 10 specimens were captured from selected sites of Bajaur Agency FATA, Pakistan using mist nets. The captured specimens were morphologically identified and various morphometric measurements were taken. The head and Body length (HB) of Pipistrellus coromondra and Pipistrellus kuhlii lepidus (n=10) was 43±0.11 mm and 45±1.1 respectively. Morphologically identified Pipistrellus kuhlii confirmed as Pipistrellus kuhlii lepidus based on 16S rRNA sequences. The DNA sequences were submitted to GenBank and accession numbers were obtained (MN 719478 and MT430902). The available 16S rRNA gene sequences of Pipistrellus coromondra and Pipistrellus kuhlii lepidus were retrieved from NCBI and incorporated in N-J tree analysis. Overall, the interspecific genetic variations among Pipistrellus coromondra and Pipistrellus kuhlii lepidus were 8% and 1% respectively. In our recommendation, a comprehensive molecular identification of bats is need of hour to report more cryptic and new species from Pakistan.

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