Screening and Identification of Selenium-tolerant Marine Strain and the Antibacterial Activity of Se Nanoparticles It Synthesized

• Main Authors: Xiaonan XU, Haodi MA, Yan XU, Xuan LI, Zhi QIN, Chunshan QUAN, Liying ZHANG
• Format: Article in Journal/Newspaper
• Language: Chinese
• Published: The editorial department of Science and Technology of Food Industry 2023
• Subjects: marine strains; se nanoparticles; biosynthesis; antibacterial activity; screening; identify; Food processing and manufacture; TP368-456; Arctic; Arctic Ocean
• Online Access: https://doi.org/10.13386/j.issn1002-0306.2023020155; https://doaj.org/article/c2476b8c948646d2b61c6be210329d83

In this study, a novel strain with high Na2SeO3 reducing ability was screened from marine strains, providing a new reduction system for the biosynthesis of Se nanoparticles (SeNPs). Firstly, 41 marine strains from Arctic Ocean sediments were screened, and a strain with high selenium-tolerant was obtained and species identified. Subsequently, the screened marine strain was used for the biosynthesis SeNPs and the SeNPs were characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and particle size analysis. Furthermore, the antibacterial activities of SeNPs were also determined. The results showed that strain 72 had the strongest tolerance to Na2SeO3, which could grow in the presence of 300 mmol/L Na2SeO3 and reducing it to brick red SeNPs. Subsequently, strain 72 was identified and named as Bacillus sp. Q72. The SeNPs biosynthesized by this strain were spherical particles with an average size of 169.3 nm and a potential of −48.1±0.5 mV. There were amino and hydroxyl groups on their surface. The SeNPs produced by Bacillus sp. Q72 showed better inhibitory activity against E. coli. When the concentration of SeNPs was 200 μg/mL, the inhibitory rate against E. coli reached 92.1%.