Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA.
BACKGROUND: Dengue virus (DENV) infections are preferentially diagnosed by detection of specific IgM antibodies, DENV NS1 antigen assays or by amplification of viral RNA in serum samples of the patients. The type-specific immunity to the four worldwide circulating DENV serotypes can be determined by...
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ftdoajarticles:oai:doaj.org/article:c1fa3aaf00db46aa918b889cc8100ee5 2023-05-15T15:16:35+02:00 Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA. Petra Emmerich Angela Mika Herbert Schmitz 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002580 https://doaj.org/article/c1fa3aaf00db46aa918b889cc8100ee5 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3873247?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002580 https://doaj.org/article/c1fa3aaf00db46aa918b889cc8100ee5 PLoS Neglected Tropical Diseases, Vol 7, Iss 12, p e2580 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002580 2022-12-31T08:17:08Z BACKGROUND: Dengue virus (DENV) infections are preferentially diagnosed by detection of specific IgM antibodies, DENV NS1 antigen assays or by amplification of viral RNA in serum samples of the patients. The type-specific immunity to the four worldwide circulating DENV serotypes can be determined by neutralization assays. An alternative to the complicated neutralization assays would be helpful to study the serotype-specific immune response in people in DENV hyperendemic areas but also in subjects upon DENV vaccination. METHODS: In consecutive samples of patients with DENV-1- 4 infection type-specific antibodies were detected using an immune complex binding (ICB) ELISA. During incubation of serum samples and enzyme- labeled recombinant envelope domain III (EDIII) antigens immune complexes (ICs) are formed, which are simultaneously bound to a solid phase coated with an Fc-receptor (CD32). After a single washing procedure the bound labeled ICs can be determined. To further improve type-specific reactions high concentrations of competing heterologous unlabeled ED III proteins were added to the labeled antigens. RESULTS: Follow-up serum samples of 64 patients with RT-PCR confirmed primary DENV-1, -2, -3 or -4 infections were tested against four enzyme-labeled recombinant DENV EDIII antigens. Antibodies to the EDIII antigens were found in 55 patients (sensitivity 86%). A complete agreement between the serotype detected by PCR in early samples and the serotype-specific antibody in later samples was found. Type-specific anti-EDIII antibodies were first detected 9-20 days after onset of the disease. In 21% of the samples collected from people in Vietnam secondary infections with antibodies to two serotypes could be identified. CONCLUSIONS: The data obtained with the ICB-ELISA show that after primary DENV infection the corresponding type-specific antibodies are detected in almost all samples collected at least two weeks after onset of the disease. The method will be of value to determine the distribution of the various ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 12 e2580 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Petra Emmerich Angela Mika Herbert Schmitz Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Dengue virus (DENV) infections are preferentially diagnosed by detection of specific IgM antibodies, DENV NS1 antigen assays or by amplification of viral RNA in serum samples of the patients. The type-specific immunity to the four worldwide circulating DENV serotypes can be determined by neutralization assays. An alternative to the complicated neutralization assays would be helpful to study the serotype-specific immune response in people in DENV hyperendemic areas but also in subjects upon DENV vaccination. METHODS: In consecutive samples of patients with DENV-1- 4 infection type-specific antibodies were detected using an immune complex binding (ICB) ELISA. During incubation of serum samples and enzyme- labeled recombinant envelope domain III (EDIII) antigens immune complexes (ICs) are formed, which are simultaneously bound to a solid phase coated with an Fc-receptor (CD32). After a single washing procedure the bound labeled ICs can be determined. To further improve type-specific reactions high concentrations of competing heterologous unlabeled ED III proteins were added to the labeled antigens. RESULTS: Follow-up serum samples of 64 patients with RT-PCR confirmed primary DENV-1, -2, -3 or -4 infections were tested against four enzyme-labeled recombinant DENV EDIII antigens. Antibodies to the EDIII antigens were found in 55 patients (sensitivity 86%). A complete agreement between the serotype detected by PCR in early samples and the serotype-specific antibody in later samples was found. Type-specific anti-EDIII antibodies were first detected 9-20 days after onset of the disease. In 21% of the samples collected from people in Vietnam secondary infections with antibodies to two serotypes could be identified. CONCLUSIONS: The data obtained with the ICB-ELISA show that after primary DENV infection the corresponding type-specific antibodies are detected in almost all samples collected at least two weeks after onset of the disease. The method will be of value to determine the distribution of the various ... |
format |
Article in Journal/Newspaper |
author |
Petra Emmerich Angela Mika Herbert Schmitz |
author_facet |
Petra Emmerich Angela Mika Herbert Schmitz |
author_sort |
Petra Emmerich |
title |
Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA. |
title_short |
Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA. |
title_full |
Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA. |
title_fullStr |
Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA. |
title_full_unstemmed |
Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA. |
title_sort |
detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (icb) elisa. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002580 https://doaj.org/article/c1fa3aaf00db46aa918b889cc8100ee5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 12, p e2580 (2013) |
op_relation |
http://europepmc.org/articles/PMC3873247?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002580 https://doaj.org/article/c1fa3aaf00db46aa918b889cc8100ee5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002580 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
12 |
container_start_page |
e2580 |
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