Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira.
NETosis is a process by which neutrophils extrude their DNA together with bactericidal proteins that trap and/or kill pathogens. In the present study, we evaluated the ability of Leptospira spp. to induce NETosis using human ex vivo and murine in vivo models. Microscopy and fluorometric studies show...
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ftdoajarticles:oai:doaj.org/article:c1f685bb01e84670ba92409976b85d56 2023-05-15T15:11:31+02:00 Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira. Emilia Scharrig Agostina Carestia María F Ferrer Maia Cédola Gabriela Pretre Ricardo Drut Mathieu Picardeau Mirta Schattner Ricardo M Gómez 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003927 https://doaj.org/article/c1f685bb01e84670ba92409976b85d56 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4498591?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003927 https://doaj.org/article/c1f685bb01e84670ba92409976b85d56 PLoS Neglected Tropical Diseases, Vol 9, Iss 7, p e0003927 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003927 2022-12-30T21:57:31Z NETosis is a process by which neutrophils extrude their DNA together with bactericidal proteins that trap and/or kill pathogens. In the present study, we evaluated the ability of Leptospira spp. to induce NETosis using human ex vivo and murine in vivo models. Microscopy and fluorometric studies showed that incubation of human neutrophils with Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 (LIC) resulted in the release of DNA extracellular traps (NETs). The bacteria number, pathogenicity and viability were relevant factors for induction of NETs, but bacteria motility was not. Entrapment of LIC in the NETs resulted in LIC death; however, pathogenic but not saprophytic Leptospira sp. exerted nuclease activity and degraded DNA. Mice infected with LIC showed circulating NETs after 2 days post-infection (dpi). Depletion of neutrophils with mAb1A8 significantly reduced the amount of intravascular NETs in LIC-infected mice, increasing bacteremia at 3 dpi. Although there was a low bacterial burden, scarce neutrophils and an absence of inflammation in the early stages of infection in the kidney and liver, at the beginning of the leptospiruric phase, the bacterial burden was significantly higher in kidneys of neutrophil-depleted-mice compared to non-depleted and infected mice. Surprisingly, interstitial nephritis was of similar intensity in both groups of infected mice. Taken together, these data suggest that LIC triggers NETs, and that the intravascular formation of these DNA traps appears to be critical not only to prevent early leptospiral dissemination but also to preclude further bacterial burden. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 7 e0003927 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Emilia Scharrig Agostina Carestia María F Ferrer Maia Cédola Gabriela Pretre Ricardo Drut Mathieu Picardeau Mirta Schattner Ricardo M Gómez Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
NETosis is a process by which neutrophils extrude their DNA together with bactericidal proteins that trap and/or kill pathogens. In the present study, we evaluated the ability of Leptospira spp. to induce NETosis using human ex vivo and murine in vivo models. Microscopy and fluorometric studies showed that incubation of human neutrophils with Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 (LIC) resulted in the release of DNA extracellular traps (NETs). The bacteria number, pathogenicity and viability were relevant factors for induction of NETs, but bacteria motility was not. Entrapment of LIC in the NETs resulted in LIC death; however, pathogenic but not saprophytic Leptospira sp. exerted nuclease activity and degraded DNA. Mice infected with LIC showed circulating NETs after 2 days post-infection (dpi). Depletion of neutrophils with mAb1A8 significantly reduced the amount of intravascular NETs in LIC-infected mice, increasing bacteremia at 3 dpi. Although there was a low bacterial burden, scarce neutrophils and an absence of inflammation in the early stages of infection in the kidney and liver, at the beginning of the leptospiruric phase, the bacterial burden was significantly higher in kidneys of neutrophil-depleted-mice compared to non-depleted and infected mice. Surprisingly, interstitial nephritis was of similar intensity in both groups of infected mice. Taken together, these data suggest that LIC triggers NETs, and that the intravascular formation of these DNA traps appears to be critical not only to prevent early leptospiral dissemination but also to preclude further bacterial burden. |
format |
Article in Journal/Newspaper |
author |
Emilia Scharrig Agostina Carestia María F Ferrer Maia Cédola Gabriela Pretre Ricardo Drut Mathieu Picardeau Mirta Schattner Ricardo M Gómez |
author_facet |
Emilia Scharrig Agostina Carestia María F Ferrer Maia Cédola Gabriela Pretre Ricardo Drut Mathieu Picardeau Mirta Schattner Ricardo M Gómez |
author_sort |
Emilia Scharrig |
title |
Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira. |
title_short |
Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira. |
title_full |
Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira. |
title_fullStr |
Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira. |
title_full_unstemmed |
Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira. |
title_sort |
neutrophil extracellular traps are involved in the innate immune response to infection with leptospira. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003927 https://doaj.org/article/c1f685bb01e84670ba92409976b85d56 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 7, p e0003927 (2015) |
op_relation |
http://europepmc.org/articles/PMC4498591?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003927 https://doaj.org/article/c1f685bb01e84670ba92409976b85d56 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003927 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
7 |
container_start_page |
e0003927 |
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1766342356071612416 |