Quarter of a Century after: A Glimpse at the Conformation and Mechanism of Candida antarctica Lipase B
Lipase B from Candida antarctica (CAL-B) belongs to the family of α/β-hydrolases, and is one from the most extensively used biocatalysts in the kinetic resolution of amines and alcohols in a racemic state, in the desymmetrization of diacetates or diols, and in the stereoselective synthesis of chiral...
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2020
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ftdoajarticles:oai:doaj.org/article:c1d6bf8b2cf64b60b5e77d7bb68b9095 2023-05-15T13:43:11+02:00 Quarter of a Century after: A Glimpse at the Conformation and Mechanism of Candida antarctica Lipase B Jarosław Błaszczyk Piotr Kiełbasiński 2020-05-01T00:00:00Z https://doi.org/10.3390/cryst10050404 https://doaj.org/article/c1d6bf8b2cf64b60b5e77d7bb68b9095 EN eng MDPI AG https://www.mdpi.com/2073-4352/10/5/404 https://doaj.org/toc/2073-4352 doi:10.3390/cryst10050404 2073-4352 https://doaj.org/article/c1d6bf8b2cf64b60b5e77d7bb68b9095 Crystals, Vol 10, Iss 404, p 404 (2020) CAL-B crystallographic polymorphism enzyme promiscuity lipase activity esterase activity macromolecular crystallography Crystallography QD901-999 article 2020 ftdoajarticles https://doi.org/10.3390/cryst10050404 2022-12-30T22:31:13Z Lipase B from Candida antarctica (CAL-B) belongs to the family of α/β-hydrolases, and is one from the most extensively used biocatalysts in the kinetic resolution of amines and alcohols in a racemic state, in the desymmetrization of diacetates or diols, and in the stereoselective synthesis of chiral intermediate compounds for obtaining the various pharmaceuticals and agents which protect plants. There are also many cases of promiscuous reactions catalyzed by CAL-B. The number of very important results appeared recently in the literature in the years 2015–2019, regarding the crystal structure and conformation of CAL-B molecule. Before 2015, there was a long period of a complete lack of information concerning this enzyme’s structure. The earlier reports about CAL-B structure were dated between 1994–1995, and did not provide enough conclusions about the mechanism of the enzyme. The recently solved structures give a hint of the enzyme mechanism in three dimensions. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Crystals 10 5 404 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
CAL-B crystallographic polymorphism enzyme promiscuity lipase activity esterase activity macromolecular crystallography Crystallography QD901-999 |
spellingShingle |
CAL-B crystallographic polymorphism enzyme promiscuity lipase activity esterase activity macromolecular crystallography Crystallography QD901-999 Jarosław Błaszczyk Piotr Kiełbasiński Quarter of a Century after: A Glimpse at the Conformation and Mechanism of Candida antarctica Lipase B |
topic_facet |
CAL-B crystallographic polymorphism enzyme promiscuity lipase activity esterase activity macromolecular crystallography Crystallography QD901-999 |
description |
Lipase B from Candida antarctica (CAL-B) belongs to the family of α/β-hydrolases, and is one from the most extensively used biocatalysts in the kinetic resolution of amines and alcohols in a racemic state, in the desymmetrization of diacetates or diols, and in the stereoselective synthesis of chiral intermediate compounds for obtaining the various pharmaceuticals and agents which protect plants. There are also many cases of promiscuous reactions catalyzed by CAL-B. The number of very important results appeared recently in the literature in the years 2015–2019, regarding the crystal structure and conformation of CAL-B molecule. Before 2015, there was a long period of a complete lack of information concerning this enzyme’s structure. The earlier reports about CAL-B structure were dated between 1994–1995, and did not provide enough conclusions about the mechanism of the enzyme. The recently solved structures give a hint of the enzyme mechanism in three dimensions. |
format |
Article in Journal/Newspaper |
author |
Jarosław Błaszczyk Piotr Kiełbasiński |
author_facet |
Jarosław Błaszczyk Piotr Kiełbasiński |
author_sort |
Jarosław Błaszczyk |
title |
Quarter of a Century after: A Glimpse at the Conformation and Mechanism of Candida antarctica Lipase B |
title_short |
Quarter of a Century after: A Glimpse at the Conformation and Mechanism of Candida antarctica Lipase B |
title_full |
Quarter of a Century after: A Glimpse at the Conformation and Mechanism of Candida antarctica Lipase B |
title_fullStr |
Quarter of a Century after: A Glimpse at the Conformation and Mechanism of Candida antarctica Lipase B |
title_full_unstemmed |
Quarter of a Century after: A Glimpse at the Conformation and Mechanism of Candida antarctica Lipase B |
title_sort |
quarter of a century after: a glimpse at the conformation and mechanism of candida antarctica lipase b |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/cryst10050404 https://doaj.org/article/c1d6bf8b2cf64b60b5e77d7bb68b9095 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Crystals, Vol 10, Iss 404, p 404 (2020) |
op_relation |
https://www.mdpi.com/2073-4352/10/5/404 https://doaj.org/toc/2073-4352 doi:10.3390/cryst10050404 2073-4352 https://doaj.org/article/c1d6bf8b2cf64b60b5e77d7bb68b9095 |
op_doi |
https://doi.org/10.3390/cryst10050404 |
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Crystals |
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10 |
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5 |
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404 |
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1766185672593375232 |