Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways.
Cystic hydatid disease is a zoonosis caused by the larval stage (hydatid) of Echinococcus granulosus (Cestoda, Taeniidae). The hydatid develops in the viscera of intermediate host as a unilocular structure filled by the hydatid fluid, which contains parasitic excretory/secretory products. The lipopr...
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ftdoajarticles:oai:doaj.org/article:c1bc05e1ef374c0cb35ec20475f61b43 2023-05-15T15:10:04+02:00 Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways. Edileuza Danieli da Silva Martin Cancela Karina Mariante Monteiro Henrique Bunselmeyer Ferreira Arnaldo Zaha 2018-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006473 https://doaj.org/article/c1bc05e1ef374c0cb35ec20475f61b43 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5955594?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006473 https://doaj.org/article/c1bc05e1ef374c0cb35ec20475f61b43 PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006473 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006473 2022-12-31T01:12:53Z Cystic hydatid disease is a zoonosis caused by the larval stage (hydatid) of Echinococcus granulosus (Cestoda, Taeniidae). The hydatid develops in the viscera of intermediate host as a unilocular structure filled by the hydatid fluid, which contains parasitic excretory/secretory products. The lipoprotein Antigen B (AgB) is the major component of E. granulosus metacestode hydatid fluid. Functionally, AgB has been implicated in immunomodulation and lipid transport. However, the mechanisms underlying AgB functions are not completely known.In this study, we investigated AgB interactions with different mammalian cell types and the pathways involved in its internalization. AgB uptake was observed in four different cell lines, NIH-3T3, A549, J774 and RH. Inhibition of caveolae/raft-mediated endocytosis causes about 50 and 69% decrease in AgB internalization by RH and A549 cells, respectively. Interestingly, AgB colocalized with the raft endocytic marker, but also showed a partial colocalization with the clathrin endocytic marker. Finally, AgB colocalized with an endolysosomal tracker, providing evidence for a possible AgB destination after endocytosis.The results indicate that caveolae/raft-mediated endocytosis is the main route to AgB internalization, and that a clathrin-mediated entry may also occur at a lower frequency. A possible fate for AgB after endocytosis seems to be the endolysosomal system. Cellular internalization and further access to subcellular compartments could be a requirement for AgB functions as a lipid carrier and/or immunomodulatory molecule, contributing to create a more permissive microenvironment to metacestode development and survival. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 5 e0006473 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Edileuza Danieli da Silva Martin Cancela Karina Mariante Monteiro Henrique Bunselmeyer Ferreira Arnaldo Zaha Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Cystic hydatid disease is a zoonosis caused by the larval stage (hydatid) of Echinococcus granulosus (Cestoda, Taeniidae). The hydatid develops in the viscera of intermediate host as a unilocular structure filled by the hydatid fluid, which contains parasitic excretory/secretory products. The lipoprotein Antigen B (AgB) is the major component of E. granulosus metacestode hydatid fluid. Functionally, AgB has been implicated in immunomodulation and lipid transport. However, the mechanisms underlying AgB functions are not completely known.In this study, we investigated AgB interactions with different mammalian cell types and the pathways involved in its internalization. AgB uptake was observed in four different cell lines, NIH-3T3, A549, J774 and RH. Inhibition of caveolae/raft-mediated endocytosis causes about 50 and 69% decrease in AgB internalization by RH and A549 cells, respectively. Interestingly, AgB colocalized with the raft endocytic marker, but also showed a partial colocalization with the clathrin endocytic marker. Finally, AgB colocalized with an endolysosomal tracker, providing evidence for a possible AgB destination after endocytosis.The results indicate that caveolae/raft-mediated endocytosis is the main route to AgB internalization, and that a clathrin-mediated entry may also occur at a lower frequency. A possible fate for AgB after endocytosis seems to be the endolysosomal system. Cellular internalization and further access to subcellular compartments could be a requirement for AgB functions as a lipid carrier and/or immunomodulatory molecule, contributing to create a more permissive microenvironment to metacestode development and survival. |
format |
Article in Journal/Newspaper |
author |
Edileuza Danieli da Silva Martin Cancela Karina Mariante Monteiro Henrique Bunselmeyer Ferreira Arnaldo Zaha |
author_facet |
Edileuza Danieli da Silva Martin Cancela Karina Mariante Monteiro Henrique Bunselmeyer Ferreira Arnaldo Zaha |
author_sort |
Edileuza Danieli da Silva |
title |
Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways. |
title_short |
Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways. |
title_full |
Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways. |
title_fullStr |
Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways. |
title_full_unstemmed |
Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways. |
title_sort |
antigen b from echinococcus granulosus enters mammalian cells by endocytic pathways. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006473 https://doaj.org/article/c1bc05e1ef374c0cb35ec20475f61b43 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006473 (2018) |
op_relation |
http://europepmc.org/articles/PMC5955594?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006473 https://doaj.org/article/c1bc05e1ef374c0cb35ec20475f61b43 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006473 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
5 |
container_start_page |
e0006473 |
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1766341138651807744 |