Helminth species-specific effects on IFN-γ producing T cells during active and latent tuberculosis.
Background Interferon-γ (IFN-γ) is a key cytokine inducing protective immune responses during tuberculosis (TB) infection. Helminth-induced immune responses may affect IFN-γ production by T cells, although its connection with disease severity and immune recovery during treatment is unexplored. We in...
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ftdoajarticles:oai:doaj.org/article:c1b1a6e2e9e44ccdb87408b5140e042a 2023-05-15T15:15:08+02:00 Helminth species-specific effects on IFN-γ producing T cells during active and latent tuberculosis. Amare Kiflie Gezahegn Bewket Fitsumbrhan Tajebe Ebba Abate Thomas Schӧn Robert Blomgran 2023-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011094 https://doaj.org/article/c1b1a6e2e9e44ccdb87408b5140e042a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011094 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011094 https://doaj.org/article/c1b1a6e2e9e44ccdb87408b5140e042a PLoS Neglected Tropical Diseases, Vol 17, Iss 1, p e0011094 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011094 2023-03-05T01:33:15Z Background Interferon-γ (IFN-γ) is a key cytokine inducing protective immune responses during tuberculosis (TB) infection. Helminth-induced immune responses may affect IFN-γ production by T cells, although its connection with disease severity and immune recovery during treatment is unexplored. We investigated the species-specific effect of helminths on the IFN-γ production by T cells in relation to disease severity during active and latent TB infection (LTBI). Methods In this study, 69 active pulmonary TB patients (PTB), 28 with LTBI and 66 healthy controls were included. Active TB was diagnosed using GenXpert MTB/RIF while QuantiFERON test (QFT) was used for the screening of healthy community controls (CCs) and for the diagnosis of LTBI. Helminth infection was identified by routine diagnosis whereas clinical disease severity was evaluated by the TB score. Intracellular IFN-γ production of T cells in stimulated peripheral blood mononuclear cells (PBMCs) was analyzed by flow cytometry using TB antigens (PPD), the polyclonal T cell activator staphylococcal enterotoxin B (SEB), or medium as unstimulated control. Results Helminth infected CCs and LTBI subjects showed a significant reduction of IFN-γ+ CD4+ T cells by PPD-stimulation compared to non-helminth infected control groups. The significant reduction in the frequency of IFN-γ+ T cells in both latent and active PTB patients following SEB stimulation was mostly attributed to Schistosoma mansoni infection, whereas Ascaris lumbricoides, Schistosoma mansoni, and hookworm infection contributed equally in CCs. Following anti-helminthic and anti-TB treatment for 2 months, the frequency of IFN-γ+ CD4 T cells in helminth coinfected PTB was restored to levels of helminth negative PTB before treatment. Helminth coinfected PTB patients with an intermediate and severe clinical course had reduced capacity for production of IFN-γ+ T cells compared to the corresponding non-helminth infected PTB. Conclusion We found a reduction in IFN-γ producing T cells by helminth coinfection ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Rif ENVELOPE(-16.172,-16.172,66.526,66.526) PLOS Neglected Tropical Diseases 17 1 e0011094 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Amare Kiflie Gezahegn Bewket Fitsumbrhan Tajebe Ebba Abate Thomas Schӧn Robert Blomgran Helminth species-specific effects on IFN-γ producing T cells during active and latent tuberculosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Interferon-γ (IFN-γ) is a key cytokine inducing protective immune responses during tuberculosis (TB) infection. Helminth-induced immune responses may affect IFN-γ production by T cells, although its connection with disease severity and immune recovery during treatment is unexplored. We investigated the species-specific effect of helminths on the IFN-γ production by T cells in relation to disease severity during active and latent TB infection (LTBI). Methods In this study, 69 active pulmonary TB patients (PTB), 28 with LTBI and 66 healthy controls were included. Active TB was diagnosed using GenXpert MTB/RIF while QuantiFERON test (QFT) was used for the screening of healthy community controls (CCs) and for the diagnosis of LTBI. Helminth infection was identified by routine diagnosis whereas clinical disease severity was evaluated by the TB score. Intracellular IFN-γ production of T cells in stimulated peripheral blood mononuclear cells (PBMCs) was analyzed by flow cytometry using TB antigens (PPD), the polyclonal T cell activator staphylococcal enterotoxin B (SEB), or medium as unstimulated control. Results Helminth infected CCs and LTBI subjects showed a significant reduction of IFN-γ+ CD4+ T cells by PPD-stimulation compared to non-helminth infected control groups. The significant reduction in the frequency of IFN-γ+ T cells in both latent and active PTB patients following SEB stimulation was mostly attributed to Schistosoma mansoni infection, whereas Ascaris lumbricoides, Schistosoma mansoni, and hookworm infection contributed equally in CCs. Following anti-helminthic and anti-TB treatment for 2 months, the frequency of IFN-γ+ CD4 T cells in helminth coinfected PTB was restored to levels of helminth negative PTB before treatment. Helminth coinfected PTB patients with an intermediate and severe clinical course had reduced capacity for production of IFN-γ+ T cells compared to the corresponding non-helminth infected PTB. Conclusion We found a reduction in IFN-γ producing T cells by helminth coinfection ... |
format |
Article in Journal/Newspaper |
author |
Amare Kiflie Gezahegn Bewket Fitsumbrhan Tajebe Ebba Abate Thomas Schӧn Robert Blomgran |
author_facet |
Amare Kiflie Gezahegn Bewket Fitsumbrhan Tajebe Ebba Abate Thomas Schӧn Robert Blomgran |
author_sort |
Amare Kiflie |
title |
Helminth species-specific effects on IFN-γ producing T cells during active and latent tuberculosis. |
title_short |
Helminth species-specific effects on IFN-γ producing T cells during active and latent tuberculosis. |
title_full |
Helminth species-specific effects on IFN-γ producing T cells during active and latent tuberculosis. |
title_fullStr |
Helminth species-specific effects on IFN-γ producing T cells during active and latent tuberculosis. |
title_full_unstemmed |
Helminth species-specific effects on IFN-γ producing T cells during active and latent tuberculosis. |
title_sort |
helminth species-specific effects on ifn-γ producing t cells during active and latent tuberculosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011094 https://doaj.org/article/c1b1a6e2e9e44ccdb87408b5140e042a |
long_lat |
ENVELOPE(-16.172,-16.172,66.526,66.526) |
geographic |
Arctic Rif |
geographic_facet |
Arctic Rif |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 1, p e0011094 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011094 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011094 https://doaj.org/article/c1b1a6e2e9e44ccdb87408b5140e042a |
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https://doi.org/10.1371/journal.pntd.0011094 |
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PLOS Neglected Tropical Diseases |
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17 |
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e0011094 |
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