Temporal stability of naturally acquired immunity to Merozoite Surface Protein-1 in Kenyan Adults
Abstract Background Naturally acquired immunity to blood-stage Plasmodium falciparum infection develops with age and after repeated infections. In order to identify immune surrogates that can inform vaccine trials conducted in malaria endemic populations and to better understand the basis of natural...
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ftdoajarticles:oai:doaj.org/article:c14f96f2473f49a4b9be53dc5bacfcd3 2023-05-15T15:14:57+02:00 Temporal stability of naturally acquired immunity to Merozoite Surface Protein-1 in Kenyan Adults Crabb Brendan S Spring Michele D Sumba Peter O Chelimo Kiprotich Dent Arlene E Moormann Ann M Tisch Daniel J Kazura James W 2009-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-8-162 https://doaj.org/article/c14f96f2473f49a4b9be53dc5bacfcd3 EN eng BMC http://www.malariajournal.com/content/8/1/162 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-162 1475-2875 https://doaj.org/article/c14f96f2473f49a4b9be53dc5bacfcd3 Malaria Journal, Vol 8, Iss 1, p 162 (2009) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1186/1475-2875-8-162 2022-12-31T08:12:37Z Abstract Background Naturally acquired immunity to blood-stage Plasmodium falciparum infection develops with age and after repeated infections. In order to identify immune surrogates that can inform vaccine trials conducted in malaria endemic populations and to better understand the basis of naturally acquired immunity it is important to appreciate the temporal stability of cellular and humoral immune responses to malaria antigens. Methods Blood samples from 16 adults living in a malaria holoendemic region of western Kenya were obtained at six time points over the course of 9 months. T cell immunity to the 42 kDa C-terminal fragment of Merozoite Surface Protein-1 (MSP-1 42 ) was determined by IFN-γ ELISPOT. Antibodies to the 42 kDa and 19 kDa C-terminal fragments of MSP-1 were determined by serology and by functional assays that measure MSP-1 19 invasion inhibition antibodies (IIA) to the E-TSR (3D7) allele and growth inhibitory activity (GIA). The haplotype of MSP-1 19 alleles circulating in the population was determined by PCR. The kappa test of agreement was used to determine stability of immunity over the specified time intervals of 3 weeks, 6 weeks, 6 months, and 9 months. Results MSP-1 IgG antibodies determined by serology were most consistent over time, followed by MSP-1 specific T cell IFN-γ responses and GIA. MSP-1 19 IIA showed the least stability over time. However, the level of MSP-1 19 specific IIA correlated with relatively higher rainfall and higher prevalence of P. falciparum infection with the MSP-1 19 E-TSR haplotype. Conclusion Variation in the stability of cellular and humoral immune responses to P. falciparum blood stage antigens needs to be considered when interpreting the significance of these measurements as immune endpoints in residents of malaria endemic regions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 8 1 162 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Crabb Brendan S Spring Michele D Sumba Peter O Chelimo Kiprotich Dent Arlene E Moormann Ann M Tisch Daniel J Kazura James W Temporal stability of naturally acquired immunity to Merozoite Surface Protein-1 in Kenyan Adults |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Naturally acquired immunity to blood-stage Plasmodium falciparum infection develops with age and after repeated infections. In order to identify immune surrogates that can inform vaccine trials conducted in malaria endemic populations and to better understand the basis of naturally acquired immunity it is important to appreciate the temporal stability of cellular and humoral immune responses to malaria antigens. Methods Blood samples from 16 adults living in a malaria holoendemic region of western Kenya were obtained at six time points over the course of 9 months. T cell immunity to the 42 kDa C-terminal fragment of Merozoite Surface Protein-1 (MSP-1 42 ) was determined by IFN-γ ELISPOT. Antibodies to the 42 kDa and 19 kDa C-terminal fragments of MSP-1 were determined by serology and by functional assays that measure MSP-1 19 invasion inhibition antibodies (IIA) to the E-TSR (3D7) allele and growth inhibitory activity (GIA). The haplotype of MSP-1 19 alleles circulating in the population was determined by PCR. The kappa test of agreement was used to determine stability of immunity over the specified time intervals of 3 weeks, 6 weeks, 6 months, and 9 months. Results MSP-1 IgG antibodies determined by serology were most consistent over time, followed by MSP-1 specific T cell IFN-γ responses and GIA. MSP-1 19 IIA showed the least stability over time. However, the level of MSP-1 19 specific IIA correlated with relatively higher rainfall and higher prevalence of P. falciparum infection with the MSP-1 19 E-TSR haplotype. Conclusion Variation in the stability of cellular and humoral immune responses to P. falciparum blood stage antigens needs to be considered when interpreting the significance of these measurements as immune endpoints in residents of malaria endemic regions. |
format |
Article in Journal/Newspaper |
author |
Crabb Brendan S Spring Michele D Sumba Peter O Chelimo Kiprotich Dent Arlene E Moormann Ann M Tisch Daniel J Kazura James W |
author_facet |
Crabb Brendan S Spring Michele D Sumba Peter O Chelimo Kiprotich Dent Arlene E Moormann Ann M Tisch Daniel J Kazura James W |
author_sort |
Crabb Brendan S |
title |
Temporal stability of naturally acquired immunity to Merozoite Surface Protein-1 in Kenyan Adults |
title_short |
Temporal stability of naturally acquired immunity to Merozoite Surface Protein-1 in Kenyan Adults |
title_full |
Temporal stability of naturally acquired immunity to Merozoite Surface Protein-1 in Kenyan Adults |
title_fullStr |
Temporal stability of naturally acquired immunity to Merozoite Surface Protein-1 in Kenyan Adults |
title_full_unstemmed |
Temporal stability of naturally acquired immunity to Merozoite Surface Protein-1 in Kenyan Adults |
title_sort |
temporal stability of naturally acquired immunity to merozoite surface protein-1 in kenyan adults |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1475-2875-8-162 https://doaj.org/article/c14f96f2473f49a4b9be53dc5bacfcd3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 8, Iss 1, p 162 (2009) |
op_relation |
http://www.malariajournal.com/content/8/1/162 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-162 1475-2875 https://doaj.org/article/c14f96f2473f49a4b9be53dc5bacfcd3 |
op_doi |
https://doi.org/10.1186/1475-2875-8-162 |
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Malaria Journal |
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8 |
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1 |
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162 |
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1766345341951541248 |