A genome-wide analysis of genetic diversity in Trypanosoma cruzi intergenic regions.
Trypanosoma cruzi is the causal agent of Chagas Disease. Recently, the genomes of representative strains from two major evolutionary lineages were sequenced, allowing the construction of a detailed genetic diversity map for this important parasite. However this map is focused on coding regions of th...
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ftdoajarticles:oai:doaj.org/article:c10f9a4b4f2242e69f69d8564116c3b4 2023-05-15T15:08:41+02:00 A genome-wide analysis of genetic diversity in Trypanosoma cruzi intergenic regions. Leonardo G Panunzi Fernán Agüero 2014-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002839 https://doaj.org/article/c10f9a4b4f2242e69f69d8564116c3b4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4006747?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002839 https://doaj.org/article/c10f9a4b4f2242e69f69d8564116c3b4 PLoS Neglected Tropical Diseases, Vol 8, Iss 5, p e2839 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002839 2022-12-31T05:27:32Z Trypanosoma cruzi is the causal agent of Chagas Disease. Recently, the genomes of representative strains from two major evolutionary lineages were sequenced, allowing the construction of a detailed genetic diversity map for this important parasite. However this map is focused on coding regions of the genome, leaving a vast space of regulatory regions uncharacterized in terms of their evolutionary conservation and/or divergence.Using data from the hybrid CL Brener and Sylvio X10 genomes (from the TcVI and TcI Discrete Typing Units, respectively), we identified intergenic regions that share a common evolutionary ancestry, and are present in both CL Brener haplotypes (TcII-like and TcIII-like) and in the TcI genome; as well as intergenic regions that were conserved in only two of the three genomes/haplotypes analyzed. The genetic diversity in these regions was characterized in terms of the accumulation of indels and nucleotide changes.Based on this analysis we have identified i) a core of highly conserved intergenic regions, which remained essentially unchanged in independently evolving lineages; ii) intergenic regions that show high diversity in spite of still retaining their corresponding upstream and downstream coding sequences; iii) a number of defined sequence motifs that are shared by a number of unrelated intergenic regions. A fraction of indels explains the diversification of some intergenic regions by the expansion/contraction of microsatellite-like repeats. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 5 e2839 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Leonardo G Panunzi Fernán Agüero A genome-wide analysis of genetic diversity in Trypanosoma cruzi intergenic regions. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trypanosoma cruzi is the causal agent of Chagas Disease. Recently, the genomes of representative strains from two major evolutionary lineages were sequenced, allowing the construction of a detailed genetic diversity map for this important parasite. However this map is focused on coding regions of the genome, leaving a vast space of regulatory regions uncharacterized in terms of their evolutionary conservation and/or divergence.Using data from the hybrid CL Brener and Sylvio X10 genomes (from the TcVI and TcI Discrete Typing Units, respectively), we identified intergenic regions that share a common evolutionary ancestry, and are present in both CL Brener haplotypes (TcII-like and TcIII-like) and in the TcI genome; as well as intergenic regions that were conserved in only two of the three genomes/haplotypes analyzed. The genetic diversity in these regions was characterized in terms of the accumulation of indels and nucleotide changes.Based on this analysis we have identified i) a core of highly conserved intergenic regions, which remained essentially unchanged in independently evolving lineages; ii) intergenic regions that show high diversity in spite of still retaining their corresponding upstream and downstream coding sequences; iii) a number of defined sequence motifs that are shared by a number of unrelated intergenic regions. A fraction of indels explains the diversification of some intergenic regions by the expansion/contraction of microsatellite-like repeats. |
format |
Article in Journal/Newspaper |
author |
Leonardo G Panunzi Fernán Agüero |
author_facet |
Leonardo G Panunzi Fernán Agüero |
author_sort |
Leonardo G Panunzi |
title |
A genome-wide analysis of genetic diversity in Trypanosoma cruzi intergenic regions. |
title_short |
A genome-wide analysis of genetic diversity in Trypanosoma cruzi intergenic regions. |
title_full |
A genome-wide analysis of genetic diversity in Trypanosoma cruzi intergenic regions. |
title_fullStr |
A genome-wide analysis of genetic diversity in Trypanosoma cruzi intergenic regions. |
title_full_unstemmed |
A genome-wide analysis of genetic diversity in Trypanosoma cruzi intergenic regions. |
title_sort |
genome-wide analysis of genetic diversity in trypanosoma cruzi intergenic regions. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0002839 https://doaj.org/article/c10f9a4b4f2242e69f69d8564116c3b4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 5, p e2839 (2014) |
op_relation |
http://europepmc.org/articles/PMC4006747?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002839 https://doaj.org/article/c10f9a4b4f2242e69f69d8564116c3b4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002839 |
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PLoS Neglected Tropical Diseases |
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8 |
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5 |
container_start_page |
e2839 |
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1766339992535171072 |