Adaptations of the antarctic bacterium Paenibacillus tundrae IMV B-7915 to copper (II) chloride exposure
Heavy metals are common in Antarctic habitats. However, the adaptations of Antarctic microorganisms to heavy metals are poorly understood. One of the mechanisms of toxicity of transition metals is the formation of free radicals which damage the cell macromolecules. In 2020, we isolated the bacteria...
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State Institution National Antarctic Scientific Center
2023
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ftdoajarticles:oai:doaj.org/article:c1067e9cb75343208768f4ddb142814b 2023-10-09T21:45:10+02:00 Adaptations of the antarctic bacterium Paenibacillus tundrae IMV B-7915 to copper (II) chloride exposure S. Komplikevych O. Maslovska T. Moravska I. Yarmoliuk N. Biront Y. Zaritska S. Hnatush 2023-08-01T00:00:00Z https://doi.org/10.33275/1727-7485.1.2023.707 https://doaj.org/article/c1067e9cb75343208768f4ddb142814b EN UK eng ukr State Institution National Antarctic Scientific Center http://uaj.uac.gov.ua/index.php/uaj/article/view/732 https://doaj.org/toc/1727-7485 https://doaj.org/toc/2415-3087 1727-7485 2415-3087 doi:10.33275/1727-7485.1.2023.707 https://doaj.org/article/c1067e9cb75343208768f4ddb142814b Український антарктичний журнал, Vol 21, Iss 1(26), Pp 66-78 (2023) antioxidant defense extracellular polymeric substances free radicals heavy metals lipid peroxidation protein damage Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 article 2023 ftdoajarticles https://doi.org/10.33275/1727-7485.1.2023.707 2023-09-17T00:37:36Z Heavy metals are common in Antarctic habitats. However, the adaptations of Antarctic microorganisms to heavy metals are poorly understood. One of the mechanisms of toxicity of transition metals is the formation of free radicals which damage the cell macromolecules. In 2020, we isolated the bacteria Paenibacillus tundrae IMV B-7915 from a sample containing moss, soil, and underground parts of Deschampsia antarctica (Berthelot Islands, Maritime Antarctic). The aim of the study was to investigate the influence of copper (II) chloride on the specific growth rate, the content of products of free radical damage to lipids and proteins, the activity of antioxidant defense system enzymes, and the synthesis of extracellular polymeric substances in P. tundrae IMV B-7915. The bacteria were incubated for an hour in Tris-HCl buffer with 2–8 mM copper (II) chloride, then washed and inoculated into the tryptic soy broth. The bacteria were cultured for 72 hours. The content of copper in the cells was determined by atomic absorption spectrometry. The content of indicators of lipid peroxidation (diene conjugates, lipid hydroperoxides, thiobarbituric acid-reactive substances), oxidative modification of proteins (carbonyl groups in proteins), the activity of the antioxidant defense system enzymes (catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione reductase), total thiols, exopolymeric compounds (exopolysaccharides and proteins) were determined photometrically. Within an hour, cells accumulate 1.5–3.4 mg Cu/g of biomass, leading to a decrease in biomass accumulation and specific growth rate within 24 hours. In cells, copper ions induce free radical reactions of damage to cell macromolecules, reflected in the increase in the content of primary lipid peroxidation products and carbonyl groups in proteins. Cell division is inhibited. In response, P. tundrae IMV B-7915 cells activate efflux systems, as evidenced by a significant decrease in copper content during prolonged cultivation, and ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Berthelot Islands Directory of Open Access Journals: DOAJ Articles Antarctic Berthelot ENVELOPE(-64.146,-64.146,-65.333,-65.333) Berthelot Islands ENVELOPE(-64.167,-64.167,-65.333,-65.333) The Antarctic Ukrainian Antarctic Journal 21 1 66 78 |
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topic |
antioxidant defense extracellular polymeric substances free radicals heavy metals lipid peroxidation protein damage Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 |
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antioxidant defense extracellular polymeric substances free radicals heavy metals lipid peroxidation protein damage Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 S. Komplikevych O. Maslovska T. Moravska I. Yarmoliuk N. Biront Y. Zaritska S. Hnatush Adaptations of the antarctic bacterium Paenibacillus tundrae IMV B-7915 to copper (II) chloride exposure |
topic_facet |
antioxidant defense extracellular polymeric substances free radicals heavy metals lipid peroxidation protein damage Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 |
description |
Heavy metals are common in Antarctic habitats. However, the adaptations of Antarctic microorganisms to heavy metals are poorly understood. One of the mechanisms of toxicity of transition metals is the formation of free radicals which damage the cell macromolecules. In 2020, we isolated the bacteria Paenibacillus tundrae IMV B-7915 from a sample containing moss, soil, and underground parts of Deschampsia antarctica (Berthelot Islands, Maritime Antarctic). The aim of the study was to investigate the influence of copper (II) chloride on the specific growth rate, the content of products of free radical damage to lipids and proteins, the activity of antioxidant defense system enzymes, and the synthesis of extracellular polymeric substances in P. tundrae IMV B-7915. The bacteria were incubated for an hour in Tris-HCl buffer with 2–8 mM copper (II) chloride, then washed and inoculated into the tryptic soy broth. The bacteria were cultured for 72 hours. The content of copper in the cells was determined by atomic absorption spectrometry. The content of indicators of lipid peroxidation (diene conjugates, lipid hydroperoxides, thiobarbituric acid-reactive substances), oxidative modification of proteins (carbonyl groups in proteins), the activity of the antioxidant defense system enzymes (catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione reductase), total thiols, exopolymeric compounds (exopolysaccharides and proteins) were determined photometrically. Within an hour, cells accumulate 1.5–3.4 mg Cu/g of biomass, leading to a decrease in biomass accumulation and specific growth rate within 24 hours. In cells, copper ions induce free radical reactions of damage to cell macromolecules, reflected in the increase in the content of primary lipid peroxidation products and carbonyl groups in proteins. Cell division is inhibited. In response, P. tundrae IMV B-7915 cells activate efflux systems, as evidenced by a significant decrease in copper content during prolonged cultivation, and ... |
format |
Article in Journal/Newspaper |
author |
S. Komplikevych O. Maslovska T. Moravska I. Yarmoliuk N. Biront Y. Zaritska S. Hnatush |
author_facet |
S. Komplikevych O. Maslovska T. Moravska I. Yarmoliuk N. Biront Y. Zaritska S. Hnatush |
author_sort |
S. Komplikevych |
title |
Adaptations of the antarctic bacterium Paenibacillus tundrae IMV B-7915 to copper (II) chloride exposure |
title_short |
Adaptations of the antarctic bacterium Paenibacillus tundrae IMV B-7915 to copper (II) chloride exposure |
title_full |
Adaptations of the antarctic bacterium Paenibacillus tundrae IMV B-7915 to copper (II) chloride exposure |
title_fullStr |
Adaptations of the antarctic bacterium Paenibacillus tundrae IMV B-7915 to copper (II) chloride exposure |
title_full_unstemmed |
Adaptations of the antarctic bacterium Paenibacillus tundrae IMV B-7915 to copper (II) chloride exposure |
title_sort |
adaptations of the antarctic bacterium paenibacillus tundrae imv b-7915 to copper (ii) chloride exposure |
publisher |
State Institution National Antarctic Scientific Center |
publishDate |
2023 |
url |
https://doi.org/10.33275/1727-7485.1.2023.707 https://doaj.org/article/c1067e9cb75343208768f4ddb142814b |
long_lat |
ENVELOPE(-64.146,-64.146,-65.333,-65.333) ENVELOPE(-64.167,-64.167,-65.333,-65.333) |
geographic |
Antarctic Berthelot Berthelot Islands The Antarctic |
geographic_facet |
Antarctic Berthelot Berthelot Islands The Antarctic |
genre |
Antarc* Antarctic Antarctica Berthelot Islands |
genre_facet |
Antarc* Antarctic Antarctica Berthelot Islands |
op_source |
Український антарктичний журнал, Vol 21, Iss 1(26), Pp 66-78 (2023) |
op_relation |
http://uaj.uac.gov.ua/index.php/uaj/article/view/732 https://doaj.org/toc/1727-7485 https://doaj.org/toc/2415-3087 1727-7485 2415-3087 doi:10.33275/1727-7485.1.2023.707 https://doaj.org/article/c1067e9cb75343208768f4ddb142814b |
op_doi |
https://doi.org/10.33275/1727-7485.1.2023.707 |
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Ukrainian Antarctic Journal |
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21 |
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1 |
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66 |
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78 |
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1779316379402371072 |