Animal model of arthritis and myositis induced by the Mayaro virus.
Background The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mecha...
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ftdoajarticles:oai:doaj.org/article:c0f04581e482452abecf4c44a3760588 2023-05-15T15:16:41+02:00 Animal model of arthritis and myositis induced by the Mayaro virus. Franciele Martins Santos Roberto Sousa Dias Michelle Dias de Oliveira Isabella Cristina Toledo Alves Costa Luciana de Souza Fernandes Carine Ribeiro Pessoa Sérgio Luis Pinto da Matta Vivian Vasconcelos Costa Danielle G Souza Cynthia Canêdo da Silva Sérgio Oliveira de Paula 2019-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007375 https://doaj.org/article/c0f04581e482452abecf4c44a3760588 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007375 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007375 https://doaj.org/article/c0f04581e482452abecf4c44a3760588 PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007375 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007375 2022-12-31T13:53:10Z Background The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mechanisms associated with MAYV-induced arthritis is of great importance due to the potential for its emergence, urbanization and dispersion to other regions. Methods 15-day old Balb/c mice were infected by two distinct pathways, below the forelimb and in the rear footpad. Animals were observed for a period of 21 days. During this time, they were monitored every 24 hours for disease signs, such as weight loss and muscle weakness. Histological damage in the muscles and joints was evaluated 3, 7, 10, 15 and 20 days post-infection. The cytokine profile in serum and muscles during MAYV infection was evaluated by flow cytometry at different post-infection times. For pain analysis, the animals were submitted to the von Frey test and titre in different organs was evaluated throughout the study to obtain viral kinetics. Findings Infection by two distinct pathways, below the forelimb and in the rear footpad, resulted in a homogeneous viral spread and the development of acute disease in animals. Clinical signs were observed such as ruffled fur, hunched posture, eye irritation and slight gait alteration. In the physical test, both groups presented loss of resistance, which was associated with histopathological damage, including myositis, arthritis, tenosynovitis and periostitis. The immune response was characterized by a strong inflammatory response mediated by the cytokines TNF-α, IL-6 and INF-γ and chemokine MCP-1, followed by the action of IL-10 and IL-4 cytokines. Interpretation The results showed that Balb/c mice represent a promising model to study mechanisms involved in MAYV pathogenesis and for future antiviral testing. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 5 e0007375 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Franciele Martins Santos Roberto Sousa Dias Michelle Dias de Oliveira Isabella Cristina Toledo Alves Costa Luciana de Souza Fernandes Carine Ribeiro Pessoa Sérgio Luis Pinto da Matta Vivian Vasconcelos Costa Danielle G Souza Cynthia Canêdo da Silva Sérgio Oliveira de Paula Animal model of arthritis and myositis induced by the Mayaro virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mechanisms associated with MAYV-induced arthritis is of great importance due to the potential for its emergence, urbanization and dispersion to other regions. Methods 15-day old Balb/c mice were infected by two distinct pathways, below the forelimb and in the rear footpad. Animals were observed for a period of 21 days. During this time, they were monitored every 24 hours for disease signs, such as weight loss and muscle weakness. Histological damage in the muscles and joints was evaluated 3, 7, 10, 15 and 20 days post-infection. The cytokine profile in serum and muscles during MAYV infection was evaluated by flow cytometry at different post-infection times. For pain analysis, the animals were submitted to the von Frey test and titre in different organs was evaluated throughout the study to obtain viral kinetics. Findings Infection by two distinct pathways, below the forelimb and in the rear footpad, resulted in a homogeneous viral spread and the development of acute disease in animals. Clinical signs were observed such as ruffled fur, hunched posture, eye irritation and slight gait alteration. In the physical test, both groups presented loss of resistance, which was associated with histopathological damage, including myositis, arthritis, tenosynovitis and periostitis. The immune response was characterized by a strong inflammatory response mediated by the cytokines TNF-α, IL-6 and INF-γ and chemokine MCP-1, followed by the action of IL-10 and IL-4 cytokines. Interpretation The results showed that Balb/c mice represent a promising model to study mechanisms involved in MAYV pathogenesis and for future antiviral testing. |
format |
Article in Journal/Newspaper |
author |
Franciele Martins Santos Roberto Sousa Dias Michelle Dias de Oliveira Isabella Cristina Toledo Alves Costa Luciana de Souza Fernandes Carine Ribeiro Pessoa Sérgio Luis Pinto da Matta Vivian Vasconcelos Costa Danielle G Souza Cynthia Canêdo da Silva Sérgio Oliveira de Paula |
author_facet |
Franciele Martins Santos Roberto Sousa Dias Michelle Dias de Oliveira Isabella Cristina Toledo Alves Costa Luciana de Souza Fernandes Carine Ribeiro Pessoa Sérgio Luis Pinto da Matta Vivian Vasconcelos Costa Danielle G Souza Cynthia Canêdo da Silva Sérgio Oliveira de Paula |
author_sort |
Franciele Martins Santos |
title |
Animal model of arthritis and myositis induced by the Mayaro virus. |
title_short |
Animal model of arthritis and myositis induced by the Mayaro virus. |
title_full |
Animal model of arthritis and myositis induced by the Mayaro virus. |
title_fullStr |
Animal model of arthritis and myositis induced by the Mayaro virus. |
title_full_unstemmed |
Animal model of arthritis and myositis induced by the Mayaro virus. |
title_sort |
animal model of arthritis and myositis induced by the mayaro virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007375 https://doaj.org/article/c0f04581e482452abecf4c44a3760588 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007375 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007375 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007375 https://doaj.org/article/c0f04581e482452abecf4c44a3760588 |
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https://doi.org/10.1371/journal.pntd.0007375 |
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PLOS Neglected Tropical Diseases |
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13 |
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5 |
container_start_page |
e0007375 |
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