Genetic polymorphism and natural selection of circumsporozoite protein in Myanmar Plasmodium vivax
Abstract Background Circumsporozoite surface protein (CSP) of malaria parasites has been recognized as one of the leading vaccine candidates. Clinical trials of vaccines for vivax malaria incorporating Plasmodium vivax CSP (PvCSP) have demonstrated their effectiveness in preventing malaria, at least...
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ftdoajarticles:oai:doaj.org/article:c0cd6e1a947b46d495f3739b7e04663e 2023-05-15T15:16:12+02:00 Genetic polymorphism and natural selection of circumsporozoite protein in Myanmar Plasmodium vivax Tuấn Cường Võ Hương Giang Lê Jung-Mi Kang Mya Moe Haung Naw Moe Kyaw Myint Jinyoung Lee Woon-Mok Sohn Tong-Soo Kim Byoung-Kuk Na 2020-09-01T00:00:00Z https://doi.org/10.1186/s12936-020-03366-7 https://doaj.org/article/c0cd6e1a947b46d495f3739b7e04663e EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03366-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03366-7 1475-2875 https://doaj.org/article/c0cd6e1a947b46d495f3739b7e04663e Malaria Journal, Vol 19, Iss 1, Pp 1-17 (2020) Plasmodium vivax Circumsporozoite protein Genetic polymorphism Natural selection Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03366-7 2022-12-31T03:24:41Z Abstract Background Circumsporozoite surface protein (CSP) of malaria parasites has been recognized as one of the leading vaccine candidates. Clinical trials of vaccines for vivax malaria incorporating Plasmodium vivax CSP (PvCSP) have demonstrated their effectiveness in preventing malaria, at least in part. However, genetic diversity of pvcsp in the natural population remains a major concern. Methods A total of 171 blood samples collected from patients infected with Plasmodium vivax in Myanmar were analysed in this study. The pvcsp was amplified by polymerase chain reaction, followed by cloning and sequencing. Polymorphic characteristics and natural selection of pvcsp population in Myanmar were analysed using DNASTAR, MEGA6 and DnaSP programs. The polymorphic pattern and natural selection of publicly accessible global pvcsp sequences were also comparatively analysed. Results Myanmar pvcsp sequences were divided into two subtypes VK210 and VK247 comprising 143 and 28 sequences, respectively. The VK210 subtypes showed higher levels of genetic diversity and polymorphism than the VK247 subtypes. The N-terminal non-repeat region of pvcsp displayed limited genetic variations in the global population. Different patterns of octapeptide insertion (ANKKAEDA in VK210 and ANKKAGDA in VK247) and tetrapeptide repeat motif (GGNA) were identified in the C-terminal region of global pvcsp population. Meanwhile, the central repeat region (CRR) of Myanmar and global pvcsp, both in VK210 and VK247 variants, was highly polymorphic. The high level of genetic diversity in the CRR has been attributed to the different numbers, types and combinations of peptide repeat motifs (PRMs). Interestingly, 27 and 5 novel PRMs were found in Myanmar VK210 and VK247 variants, respectively. Conclusion Comparative analysis of the global pvcsp population suggests a complex genetic profile of pvcsp in the global population. These results widen understanding of the genetic make-up of pvcsp in the global P. vivax population and provide valuable ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Plasmodium vivax Circumsporozoite protein Genetic polymorphism Natural selection Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium vivax Circumsporozoite protein Genetic polymorphism Natural selection Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Tuấn Cường Võ Hương Giang Lê Jung-Mi Kang Mya Moe Haung Naw Moe Kyaw Myint Jinyoung Lee Woon-Mok Sohn Tong-Soo Kim Byoung-Kuk Na Genetic polymorphism and natural selection of circumsporozoite protein in Myanmar Plasmodium vivax |
topic_facet |
Plasmodium vivax Circumsporozoite protein Genetic polymorphism Natural selection Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Circumsporozoite surface protein (CSP) of malaria parasites has been recognized as one of the leading vaccine candidates. Clinical trials of vaccines for vivax malaria incorporating Plasmodium vivax CSP (PvCSP) have demonstrated their effectiveness in preventing malaria, at least in part. However, genetic diversity of pvcsp in the natural population remains a major concern. Methods A total of 171 blood samples collected from patients infected with Plasmodium vivax in Myanmar were analysed in this study. The pvcsp was amplified by polymerase chain reaction, followed by cloning and sequencing. Polymorphic characteristics and natural selection of pvcsp population in Myanmar were analysed using DNASTAR, MEGA6 and DnaSP programs. The polymorphic pattern and natural selection of publicly accessible global pvcsp sequences were also comparatively analysed. Results Myanmar pvcsp sequences were divided into two subtypes VK210 and VK247 comprising 143 and 28 sequences, respectively. The VK210 subtypes showed higher levels of genetic diversity and polymorphism than the VK247 subtypes. The N-terminal non-repeat region of pvcsp displayed limited genetic variations in the global population. Different patterns of octapeptide insertion (ANKKAEDA in VK210 and ANKKAGDA in VK247) and tetrapeptide repeat motif (GGNA) were identified in the C-terminal region of global pvcsp population. Meanwhile, the central repeat region (CRR) of Myanmar and global pvcsp, both in VK210 and VK247 variants, was highly polymorphic. The high level of genetic diversity in the CRR has been attributed to the different numbers, types and combinations of peptide repeat motifs (PRMs). Interestingly, 27 and 5 novel PRMs were found in Myanmar VK210 and VK247 variants, respectively. Conclusion Comparative analysis of the global pvcsp population suggests a complex genetic profile of pvcsp in the global population. These results widen understanding of the genetic make-up of pvcsp in the global P. vivax population and provide valuable ... |
format |
Article in Journal/Newspaper |
author |
Tuấn Cường Võ Hương Giang Lê Jung-Mi Kang Mya Moe Haung Naw Moe Kyaw Myint Jinyoung Lee Woon-Mok Sohn Tong-Soo Kim Byoung-Kuk Na |
author_facet |
Tuấn Cường Võ Hương Giang Lê Jung-Mi Kang Mya Moe Haung Naw Moe Kyaw Myint Jinyoung Lee Woon-Mok Sohn Tong-Soo Kim Byoung-Kuk Na |
author_sort |
Tuấn Cường Võ |
title |
Genetic polymorphism and natural selection of circumsporozoite protein in Myanmar Plasmodium vivax |
title_short |
Genetic polymorphism and natural selection of circumsporozoite protein in Myanmar Plasmodium vivax |
title_full |
Genetic polymorphism and natural selection of circumsporozoite protein in Myanmar Plasmodium vivax |
title_fullStr |
Genetic polymorphism and natural selection of circumsporozoite protein in Myanmar Plasmodium vivax |
title_full_unstemmed |
Genetic polymorphism and natural selection of circumsporozoite protein in Myanmar Plasmodium vivax |
title_sort |
genetic polymorphism and natural selection of circumsporozoite protein in myanmar plasmodium vivax |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03366-7 https://doaj.org/article/c0cd6e1a947b46d495f3739b7e04663e |
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Arctic |
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Arctic |
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Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-17 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03366-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03366-7 1475-2875 https://doaj.org/article/c0cd6e1a947b46d495f3739b7e04663e |
op_doi |
https://doi.org/10.1186/s12936-020-03366-7 |
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Malaria Journal |
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19 |
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1766346496592052224 |