Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea
A Gram-negative, aerobic, rod-shaped, motile bacterium designated KMM 9957 T was isolated from a bottom sediment sample obtained from the Okhotsk Sea, Russia. Phylogenetic analyses based on the 16S rRNA gene and concatenated conserved protein-coding sequences positioned the novel strain KMM 9957 T i...
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2022
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ftdoajarticles:oai:doaj.org/article:c0cbf7a6a2c74efda1490a5f5ab0221c 2023-05-15T17:52:35+02:00 Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea Lyudmila Romanenko Nadezhda Otstavnykh Valeriya Kurilenko Peter Velansky Sergey Baldaev Valery Mikhailov Marina Isaeva 2022-11-01T00:00:00Z https://doi.org/10.3390/d14110944 https://doaj.org/article/c0cbf7a6a2c74efda1490a5f5ab0221c EN eng MDPI AG https://www.mdpi.com/1424-2818/14/11/944 https://doaj.org/toc/1424-2818 doi:10.3390/d14110944 1424-2818 https://doaj.org/article/c0cbf7a6a2c74efda1490a5f5ab0221c Diversity, Vol 14, Iss 944, p 944 (2022) Marinobacterium sedimentorum sp. nov Gammaproteobacteria marine bacteria bottom sediments the Okhotsk Sea Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/d14110944 2022-12-30T20:09:20Z A Gram-negative, aerobic, rod-shaped, motile bacterium designated KMM 9957 T was isolated from a bottom sediment sample obtained from the Okhotsk Sea, Russia. Phylogenetic analyses based on the 16S rRNA gene and concatenated conserved protein-coding sequences positioned the novel strain KMM 9957 T in the genus Marinobacterium as a distinct line adjacent to Marinobacterium rhizophilum CL-YJ9 T , Marinobacterium profundum PAMC 27536 T , and Marinobacterium aestuarii KCTC 52193 T with 16S rRNA gene sequence similarities of 99%, 98.3%, and 98.2%, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strain KMM 9957 T and M. aestuarii KCTC 52193 T , M. profundum PAMC 27536 T , and M. rhizophilum DSM 18822 T were 89.4%, 87.9%, and 84.7% and 38.6%, 34.8%, and 28.4%, respectively. The genomic DNA G+C content of strain KMM 9957 T was 58.4 mol%. The dominant respiratory quinone was ubiquinone Q-8, and the major fatty acids were C 18:1 , C 10:0 3-OH, C 16:0 , and C 16:1 ω 7 c . The polar lipids of strain KMM 9957 T consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminophospholipid, two unidentified aminolipids, eight unidentified phospholipids, and three unidentified lipids. Based on the combination of phylogenetic and phenotypic characteristics, strain KMM 9957 T represents a novel species of the genus Marinobacterium , for which the name Marinobacterium sedimentorum sp. nov. is proposed. Article in Journal/Newspaper okhotsk sea Directory of Open Access Journals: DOAJ Articles Okhotsk Diversity 14 11 944 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Marinobacterium sedimentorum sp. nov Gammaproteobacteria marine bacteria bottom sediments the Okhotsk Sea Biology (General) QH301-705.5 |
spellingShingle |
Marinobacterium sedimentorum sp. nov Gammaproteobacteria marine bacteria bottom sediments the Okhotsk Sea Biology (General) QH301-705.5 Lyudmila Romanenko Nadezhda Otstavnykh Valeriya Kurilenko Peter Velansky Sergey Baldaev Valery Mikhailov Marina Isaeva Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea |
topic_facet |
Marinobacterium sedimentorum sp. nov Gammaproteobacteria marine bacteria bottom sediments the Okhotsk Sea Biology (General) QH301-705.5 |
description |
A Gram-negative, aerobic, rod-shaped, motile bacterium designated KMM 9957 T was isolated from a bottom sediment sample obtained from the Okhotsk Sea, Russia. Phylogenetic analyses based on the 16S rRNA gene and concatenated conserved protein-coding sequences positioned the novel strain KMM 9957 T in the genus Marinobacterium as a distinct line adjacent to Marinobacterium rhizophilum CL-YJ9 T , Marinobacterium profundum PAMC 27536 T , and Marinobacterium aestuarii KCTC 52193 T with 16S rRNA gene sequence similarities of 99%, 98.3%, and 98.2%, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strain KMM 9957 T and M. aestuarii KCTC 52193 T , M. profundum PAMC 27536 T , and M. rhizophilum DSM 18822 T were 89.4%, 87.9%, and 84.7% and 38.6%, 34.8%, and 28.4%, respectively. The genomic DNA G+C content of strain KMM 9957 T was 58.4 mol%. The dominant respiratory quinone was ubiquinone Q-8, and the major fatty acids were C 18:1 , C 10:0 3-OH, C 16:0 , and C 16:1 ω 7 c . The polar lipids of strain KMM 9957 T consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminophospholipid, two unidentified aminolipids, eight unidentified phospholipids, and three unidentified lipids. Based on the combination of phylogenetic and phenotypic characteristics, strain KMM 9957 T represents a novel species of the genus Marinobacterium , for which the name Marinobacterium sedimentorum sp. nov. is proposed. |
format |
Article in Journal/Newspaper |
author |
Lyudmila Romanenko Nadezhda Otstavnykh Valeriya Kurilenko Peter Velansky Sergey Baldaev Valery Mikhailov Marina Isaeva |
author_facet |
Lyudmila Romanenko Nadezhda Otstavnykh Valeriya Kurilenko Peter Velansky Sergey Baldaev Valery Mikhailov Marina Isaeva |
author_sort |
Lyudmila Romanenko |
title |
Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea |
title_short |
Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea |
title_full |
Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea |
title_fullStr |
Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea |
title_full_unstemmed |
Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea |
title_sort |
marinobacterium sedimentorum sp. nov., isolated from the bottom sediments of the okhotsk sea |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/d14110944 https://doaj.org/article/c0cbf7a6a2c74efda1490a5f5ab0221c |
geographic |
Okhotsk |
geographic_facet |
Okhotsk |
genre |
okhotsk sea |
genre_facet |
okhotsk sea |
op_source |
Diversity, Vol 14, Iss 944, p 944 (2022) |
op_relation |
https://www.mdpi.com/1424-2818/14/11/944 https://doaj.org/toc/1424-2818 doi:10.3390/d14110944 1424-2818 https://doaj.org/article/c0cbf7a6a2c74efda1490a5f5ab0221c |
op_doi |
https://doi.org/10.3390/d14110944 |
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Diversity |
container_volume |
14 |
container_issue |
11 |
container_start_page |
944 |
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1766160188440576000 |