Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea

A Gram-negative, aerobic, rod-shaped, motile bacterium designated KMM 9957 T was isolated from a bottom sediment sample obtained from the Okhotsk Sea, Russia. Phylogenetic analyses based on the 16S rRNA gene and concatenated conserved protein-coding sequences positioned the novel strain KMM 9957 T i...

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Bibliographic Details
Published in:Diversity
Main Authors: Lyudmila Romanenko, Nadezhda Otstavnykh, Valeriya Kurilenko, Peter Velansky, Sergey Baldaev, Valery Mikhailov, Marina Isaeva
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2022
Subjects:
Marinobacterium sedimentorum sp. nov
Gammaproteobacteria
marine bacteria
bottom sediments
the Okhotsk Sea
Biology (General)
QH301-705.5
Okhotsk
okhotsk sea
Online Access:https://doi.org/10.3390/d14110944
https://doaj.org/article/c0cbf7a6a2c74efda1490a5f5ab0221c
Description
Summary:A Gram-negative, aerobic, rod-shaped, motile bacterium designated KMM 9957 T was isolated from a bottom sediment sample obtained from the Okhotsk Sea, Russia. Phylogenetic analyses based on the 16S rRNA gene and concatenated conserved protein-coding sequences positioned the novel strain KMM 9957 T in the genus Marinobacterium as a distinct line adjacent to Marinobacterium rhizophilum CL-YJ9 T , Marinobacterium profundum PAMC 27536 T , and Marinobacterium aestuarii KCTC 52193 T with 16S rRNA gene sequence similarities of 99%, 98.3%, and 98.2%, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strain KMM 9957 T and M. aestuarii KCTC 52193 T , M. profundum PAMC 27536 T , and M. rhizophilum DSM 18822 T were 89.4%, 87.9%, and 84.7% and 38.6%, 34.8%, and 28.4%, respectively. The genomic DNA G+C content of strain KMM 9957 T was 58.4 mol%. The dominant respiratory quinone was ubiquinone Q-8, and the major fatty acids were C 18:1 , C 10:0 3-OH, C 16:0 , and C 16:1 ω 7 c . The polar lipids of strain KMM 9957 T consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminophospholipid, two unidentified aminolipids, eight unidentified phospholipids, and three unidentified lipids. Based on the combination of phylogenetic and phenotypic characteristics, strain KMM 9957 T represents a novel species of the genus Marinobacterium , for which the name Marinobacterium sedimentorum sp. nov. is proposed.

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