In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection.
Humans who experience a primary dengue virus (DENV) infection develop antibodies that preferentially neutralize the homologous serotype responsible for infection. Affected individuals also generate cross-reactive antibodies against heterologous DENV serotypes, which are non-neutralizing. Dengue cros...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0001188 https://doaj.org/article/c08c8e82c5f44d798389b5216ae750e4 |
id |
ftdoajarticles:oai:doaj.org/article:c08c8e82c5f44d798389b5216ae750e4 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:c08c8e82c5f44d798389b5216ae750e4 2023-05-15T15:07:24+02:00 In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection. Ruklanthi de Alwis Martina Beltramello William B Messer Soila Sukupolvi-Petty Wahala M P B Wahala Annette Kraus Nicholas P Olivarez Quang Pham James D Brien Wen-Yang Tsai Wei-Kung Wang Scott Halstead Srisakul Kliks Michael S Diamond Ralph Baric Antonio Lanzavecchia Federica Sallusto Aravinda M de Silva 2011-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001188 https://doaj.org/article/c08c8e82c5f44d798389b5216ae750e4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3119640?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001188 https://doaj.org/article/c08c8e82c5f44d798389b5216ae750e4 PLoS Neglected Tropical Diseases, Vol 5, Iss 6, p e1188 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001188 2022-12-31T01:48:33Z Humans who experience a primary dengue virus (DENV) infection develop antibodies that preferentially neutralize the homologous serotype responsible for infection. Affected individuals also generate cross-reactive antibodies against heterologous DENV serotypes, which are non-neutralizing. Dengue cross-reactive, non-neutralizing antibodies can enhance infection of Fc receptor bearing cells and, potentially, exacerbate disease. The actual binding sites of human antibody on the DENV particle are not well defined. We characterized the specificity and neutralization potency of polyclonal serum antibodies and memory B-cell derived monoclonal antibodies (hMAbs) from 2 individuals exposed to primary DENV infections. Most DENV-specific hMAbs were serotype cross-reactive and weakly neutralizing. Moreover, many hMAbs bound to the viral pre-membrane protein and other sites on the virus that were not preserved when the viral envelope protein was produced as a soluble, recombinant antigen (rE protein). Nonetheless, by modifying the screening procedure to detect rare antibodies that bound to rE, we were able to isolate and map human antibodies that strongly neutralized the homologous serotype of DENV. Our MAbs results indicate that, in these two individuals exposed to primary DENV infections, a small fraction of the total antibody response was responsible for virus neutralization. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 6 e1188 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ruklanthi de Alwis Martina Beltramello William B Messer Soila Sukupolvi-Petty Wahala M P B Wahala Annette Kraus Nicholas P Olivarez Quang Pham James D Brien Wen-Yang Tsai Wei-Kung Wang Scott Halstead Srisakul Kliks Michael S Diamond Ralph Baric Antonio Lanzavecchia Federica Sallusto Aravinda M de Silva In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Humans who experience a primary dengue virus (DENV) infection develop antibodies that preferentially neutralize the homologous serotype responsible for infection. Affected individuals also generate cross-reactive antibodies against heterologous DENV serotypes, which are non-neutralizing. Dengue cross-reactive, non-neutralizing antibodies can enhance infection of Fc receptor bearing cells and, potentially, exacerbate disease. The actual binding sites of human antibody on the DENV particle are not well defined. We characterized the specificity and neutralization potency of polyclonal serum antibodies and memory B-cell derived monoclonal antibodies (hMAbs) from 2 individuals exposed to primary DENV infections. Most DENV-specific hMAbs were serotype cross-reactive and weakly neutralizing. Moreover, many hMAbs bound to the viral pre-membrane protein and other sites on the virus that were not preserved when the viral envelope protein was produced as a soluble, recombinant antigen (rE protein). Nonetheless, by modifying the screening procedure to detect rare antibodies that bound to rE, we were able to isolate and map human antibodies that strongly neutralized the homologous serotype of DENV. Our MAbs results indicate that, in these two individuals exposed to primary DENV infections, a small fraction of the total antibody response was responsible for virus neutralization. |
format |
Article in Journal/Newspaper |
author |
Ruklanthi de Alwis Martina Beltramello William B Messer Soila Sukupolvi-Petty Wahala M P B Wahala Annette Kraus Nicholas P Olivarez Quang Pham James D Brien Wen-Yang Tsai Wei-Kung Wang Scott Halstead Srisakul Kliks Michael S Diamond Ralph Baric Antonio Lanzavecchia Federica Sallusto Aravinda M de Silva |
author_facet |
Ruklanthi de Alwis Martina Beltramello William B Messer Soila Sukupolvi-Petty Wahala M P B Wahala Annette Kraus Nicholas P Olivarez Quang Pham James D Brien Wen-Yang Tsai Wei-Kung Wang Scott Halstead Srisakul Kliks Michael S Diamond Ralph Baric Antonio Lanzavecchia Federica Sallusto Aravinda M de Silva |
author_sort |
Ruklanthi de Alwis |
title |
In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection. |
title_short |
In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection. |
title_full |
In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection. |
title_fullStr |
In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection. |
title_full_unstemmed |
In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection. |
title_sort |
in-depth analysis of the antibody response of individuals exposed to primary dengue virus infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001188 https://doaj.org/article/c08c8e82c5f44d798389b5216ae750e4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 6, p e1188 (2011) |
op_relation |
http://europepmc.org/articles/PMC3119640?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001188 https://doaj.org/article/c08c8e82c5f44d798389b5216ae750e4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001188 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
6 |
container_start_page |
e1188 |
_version_ |
1766338916115283968 |