Metabolic profiling of Antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy
The continent of Antarctica, as a combination of constantly low temperatures, strong winds, short summer season, and high solar radiation, is a highly extreme habitat suggesting appropriate conditions for growth of psychrophilic microorganisms. Five psychrophilic yeast strains were isolated from the...
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ftdoajarticles:oai:doaj.org/article:c051c02ef11a455aa0b3391160d95c4c 2023-05-15T13:45:56+02:00 Metabolic profiling of Antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy Snezhana Rusinova-Videva Margarita Kambourova Kalina Alipieva Stefka Nachkova Svetlana Simova 2019-01-01T00:00:00Z https://doi.org/10.1080/13102818.2018.1490201 https://doaj.org/article/c051c02ef11a455aa0b3391160d95c4c EN eng Taylor & Francis Group http://dx.doi.org/10.1080/13102818.2018.1490201 https://doaj.org/toc/1310-2818 https://doaj.org/toc/1314-3530 1310-2818 1314-3530 doi:10.1080/13102818.2018.1490201 https://doaj.org/article/c051c02ef11a455aa0b3391160d95c4c Biotechnology & Biotechnological Equipment, Vol 33, Iss 1, Pp 12-19 (2019) antarctic yeasts nmr metabolite profiling Biotechnology TP248.13-248.65 article 2019 ftdoajarticles https://doi.org/10.1080/13102818.2018.1490201 2022-12-31T12:48:24Z The continent of Antarctica, as a combination of constantly low temperatures, strong winds, short summer season, and high solar radiation, is a highly extreme habitat suggesting appropriate conditions for growth of psychrophilic microorganisms. Five psychrophilic yeast strains were isolated from the samples taken from the region of the Bulgarian Base on Livingston Island, Antarctica: Cryptococcus laurentii AL65, Sporobolomyces salmonicolor AL36, Debaryomyces hansenii, Leucosporidium scotii and Rhodotorula glutinis, and their biomass yield and exopolysaccharides production were investigated. Best growth was observed for L. scotii and C. laurentii AL65, with 7.5 and 6.0 g/L biomass, respectively, and highest exopolysaccharide yield was established for L. scotii. Metabolic profiling revealed phylogenetically based diversity in the identified metabolic profiles. The proton nuclear magnetic resonance (1H NMR) spectroscopy analyses of compounds extracted from the biomass of the strains revealed significant differences in the metabolites between individual yeast strains in our investigation including: among the amino acids alanine, valine, threonine, leucine and tyrosine; some organic acids such as gamma-aminobutyric acid, acetic acid, formic acid and others. Glucose was identified in all investigated strains. The highest diversity of compounds was observed in D. hansenii strain, division Ascomycota. The main compounds in the metabolic profile of Basidiomycota strains were sugars. The statistical analysis revealed significant differences in the studied metabolites among the yeast strains. This result suggests that, together with 16S rRNA gene and enzyme gene analyses, metabolite profiling could be also used as a marker for a phylogenetic distance in fungi evolution. Article in Journal/Newspaper Antarc* Antarctic Antarctica Livingston Island Directory of Open Access Journals: DOAJ Articles Antarctic Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) Biotechnology & Biotechnological Equipment 33 1 12 19 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antarctic yeasts nmr metabolite profiling Biotechnology TP248.13-248.65 |
spellingShingle |
antarctic yeasts nmr metabolite profiling Biotechnology TP248.13-248.65 Snezhana Rusinova-Videva Margarita Kambourova Kalina Alipieva Stefka Nachkova Svetlana Simova Metabolic profiling of Antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy |
topic_facet |
antarctic yeasts nmr metabolite profiling Biotechnology TP248.13-248.65 |
description |
The continent of Antarctica, as a combination of constantly low temperatures, strong winds, short summer season, and high solar radiation, is a highly extreme habitat suggesting appropriate conditions for growth of psychrophilic microorganisms. Five psychrophilic yeast strains were isolated from the samples taken from the region of the Bulgarian Base on Livingston Island, Antarctica: Cryptococcus laurentii AL65, Sporobolomyces salmonicolor AL36, Debaryomyces hansenii, Leucosporidium scotii and Rhodotorula glutinis, and their biomass yield and exopolysaccharides production were investigated. Best growth was observed for L. scotii and C. laurentii AL65, with 7.5 and 6.0 g/L biomass, respectively, and highest exopolysaccharide yield was established for L. scotii. Metabolic profiling revealed phylogenetically based diversity in the identified metabolic profiles. The proton nuclear magnetic resonance (1H NMR) spectroscopy analyses of compounds extracted from the biomass of the strains revealed significant differences in the metabolites between individual yeast strains in our investigation including: among the amino acids alanine, valine, threonine, leucine and tyrosine; some organic acids such as gamma-aminobutyric acid, acetic acid, formic acid and others. Glucose was identified in all investigated strains. The highest diversity of compounds was observed in D. hansenii strain, division Ascomycota. The main compounds in the metabolic profile of Basidiomycota strains were sugars. The statistical analysis revealed significant differences in the studied metabolites among the yeast strains. This result suggests that, together with 16S rRNA gene and enzyme gene analyses, metabolite profiling could be also used as a marker for a phylogenetic distance in fungi evolution. |
format |
Article in Journal/Newspaper |
author |
Snezhana Rusinova-Videva Margarita Kambourova Kalina Alipieva Stefka Nachkova Svetlana Simova |
author_facet |
Snezhana Rusinova-Videva Margarita Kambourova Kalina Alipieva Stefka Nachkova Svetlana Simova |
author_sort |
Snezhana Rusinova-Videva |
title |
Metabolic profiling of Antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy |
title_short |
Metabolic profiling of Antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy |
title_full |
Metabolic profiling of Antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy |
title_fullStr |
Metabolic profiling of Antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy |
title_full_unstemmed |
Metabolic profiling of Antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy |
title_sort |
metabolic profiling of antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy |
publisher |
Taylor & Francis Group |
publishDate |
2019 |
url |
https://doi.org/10.1080/13102818.2018.1490201 https://doaj.org/article/c051c02ef11a455aa0b3391160d95c4c |
long_lat |
ENVELOPE(-60.500,-60.500,-62.600,-62.600) |
geographic |
Antarctic Livingston Island |
geographic_facet |
Antarctic Livingston Island |
genre |
Antarc* Antarctic Antarctica Livingston Island |
genre_facet |
Antarc* Antarctic Antarctica Livingston Island |
op_source |
Biotechnology & Biotechnological Equipment, Vol 33, Iss 1, Pp 12-19 (2019) |
op_relation |
http://dx.doi.org/10.1080/13102818.2018.1490201 https://doaj.org/toc/1310-2818 https://doaj.org/toc/1314-3530 1310-2818 1314-3530 doi:10.1080/13102818.2018.1490201 https://doaj.org/article/c051c02ef11a455aa0b3391160d95c4c |
op_doi |
https://doi.org/10.1080/13102818.2018.1490201 |
container_title |
Biotechnology & Biotechnological Equipment |
container_volume |
33 |
container_issue |
1 |
container_start_page |
12 |
op_container_end_page |
19 |
_version_ |
1766232788024950784 |