Molecular approaches to determine the multiplicity of Plasmodium infections

Abstract Multiplicity of infection (MOI), also termed complexity of infection (COI), is defined as the number of genetically distinct parasite strains co-infecting a single host, which is an important indicator of malaria epidemiology. PCR-based genotyping often underestimates MOI. Next generation s...

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Bibliographic Details
Published in:Malaria Journal
Main Authors: Daibin Zhong, Cristian Koepfli, Liwang Cui, Guiyun Yan
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2018
Subjects:
Malaria parasite
Multiplicity of infection
Molecular marker
Next generation sequencing
Amplicon ultra-deep sequencing
Single nucleotide polymorphism
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1186/s12936-018-2322-5
https://doaj.org/article/c028edf13efa41b68f446eba987f1b77
Description
Summary:Abstract Multiplicity of infection (MOI), also termed complexity of infection (COI), is defined as the number of genetically distinct parasite strains co-infecting a single host, which is an important indicator of malaria epidemiology. PCR-based genotyping often underestimates MOI. Next generation sequencing technologies provide much more accurate and genome-wide characterization of polyclonal infections. However, complete haplotype characterization of multiclonal infections remains a challenge due to PCR artifacts and sequencing errors, and requires efficient computational tools. In this review, the advantages and limitations of current molecular approaches to determine multiplicity of malaria parasite infection are discussed.

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