Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1
Objective: To determine the effects of toll-like receptor 2 (TLR-2) agonist, Pam3CSK4, on cellular and humoral immune response against recombinant Mycobacterium bovis bacille Calmette-Guérin (rBCG) expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum. Methods: Six groups...
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Wolters Kluwer Medknow Publications
2019
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Online Access: | https://doi.org/10.4103/2221-1691.261742 https://doaj.org/article/c01e25e849e3428e8af8c3153a146cd4 |
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ftdoajarticles:oai:doaj.org/article:c01e25e849e3428e8af8c3153a146cd4 2023-05-15T15:09:19+02:00 Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1 Mohamed H Abdikarim Muhammad A Abbas Munirah N Zakaria Robaiza Zakaria Rapeah Suppian 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.261742 https://doaj.org/article/c01e25e849e3428e8af8c3153a146cd4 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=7;spage=271;epage=277;aulast=Abdikarim https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.261742 https://doaj.org/article/c01e25e849e3428e8af8c3153a146cd4 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 7, Pp 271-277 (2019) adjuvant rbcg immune msp-1 tlr-2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.261742 2022-12-31T09:50:15Z Objective: To determine the effects of toll-like receptor 2 (TLR-2) agonist, Pam3CSK4, on cellular and humoral immune response against recombinant Mycobacterium bovis bacille Calmette-Guérin (rBCG) expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum. Methods: Six groups of mice (n=6 per group) received intraperitoneal phosphate buffered saline T80 (PBS-T80), BCG or rBCG in the presence or absence of Pam3CSK4. Enzyme- linked immunosorbent assay was carried out to measure serum total IgG, IgG1, IgG2a, and IgG2b production. Spleens were also harvested and splenocytes were co-cultured with rBCG antigen for in vitro determination of IL-4 and IFN- γ via enzyme-linked immunosorbent assay. Results: The production of total IgG and the isotype IgG1, IgG2a and IgG2b was significantly higher in rBCG-immunised mice than in the BCG and PBS-T80-immunised mice, and Pam3CSK4 further enhanced their productions. A similar pattern was also observed in IFN- γ production. Moreover, there was no significant difference in IL-4 production in all groups either in the presence or absence of Pam3CSK4. Conclusions: We present evidence of the adjuvant effects of TLR-2 agonist in enhancing the production of total IgG, IgG1, IgG2a, IgG2b, as well as IFN- γ in response to rBCG. However, the presence or absence of Pam3CSK4 had no effect on IL-4 production. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Calmette ENVELOPE(-67.150,-67.150,-68.050,-68.050) Asian Pacific Journal of Tropical Biomedicine 9 7 271 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
adjuvant rbcg immune msp-1 tlr-2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
adjuvant rbcg immune msp-1 tlr-2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Mohamed H Abdikarim Muhammad A Abbas Munirah N Zakaria Robaiza Zakaria Rapeah Suppian Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1 |
topic_facet |
adjuvant rbcg immune msp-1 tlr-2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To determine the effects of toll-like receptor 2 (TLR-2) agonist, Pam3CSK4, on cellular and humoral immune response against recombinant Mycobacterium bovis bacille Calmette-Guérin (rBCG) expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum. Methods: Six groups of mice (n=6 per group) received intraperitoneal phosphate buffered saline T80 (PBS-T80), BCG or rBCG in the presence or absence of Pam3CSK4. Enzyme- linked immunosorbent assay was carried out to measure serum total IgG, IgG1, IgG2a, and IgG2b production. Spleens were also harvested and splenocytes were co-cultured with rBCG antigen for in vitro determination of IL-4 and IFN- γ via enzyme-linked immunosorbent assay. Results: The production of total IgG and the isotype IgG1, IgG2a and IgG2b was significantly higher in rBCG-immunised mice than in the BCG and PBS-T80-immunised mice, and Pam3CSK4 further enhanced their productions. A similar pattern was also observed in IFN- γ production. Moreover, there was no significant difference in IL-4 production in all groups either in the presence or absence of Pam3CSK4. Conclusions: We present evidence of the adjuvant effects of TLR-2 agonist in enhancing the production of total IgG, IgG1, IgG2a, IgG2b, as well as IFN- γ in response to rBCG. However, the presence or absence of Pam3CSK4 had no effect on IL-4 production. |
format |
Article in Journal/Newspaper |
author |
Mohamed H Abdikarim Muhammad A Abbas Munirah N Zakaria Robaiza Zakaria Rapeah Suppian |
author_facet |
Mohamed H Abdikarim Muhammad A Abbas Munirah N Zakaria Robaiza Zakaria Rapeah Suppian |
author_sort |
Mohamed H Abdikarim |
title |
Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1 |
title_short |
Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1 |
title_full |
Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1 |
title_fullStr |
Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1 |
title_full_unstemmed |
Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1 |
title_sort |
pam3csk4 enhances adaptive immune responses to recombinant mycobacterium bovis bacille calmette-guérin expressing plasmodium falciparum c-terminus merozoite surface protein-1 |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.261742 https://doaj.org/article/c01e25e849e3428e8af8c3153a146cd4 |
long_lat |
ENVELOPE(-67.150,-67.150,-68.050,-68.050) |
geographic |
Arctic Calmette |
geographic_facet |
Arctic Calmette |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 7, Pp 271-277 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=7;spage=271;epage=277;aulast=Abdikarim https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.261742 https://doaj.org/article/c01e25e849e3428e8af8c3153a146cd4 |
op_doi |
https://doi.org/10.4103/2221-1691.261742 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
7 |
container_start_page |
271 |
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1766340517960876032 |