Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection.
Previous works showed that immunization with saliva from Lutzomyia intermedia, a vector of Leishmania braziliensis, does not protect against experimental infection. However, L. braziliensis is also transmitted by Lutzomyia whitmani, a sand fly species closely related to Lu. intermedia. Herein we des...
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ftdoajarticles:oai:doaj.org/article:c000fe0595114c408484917f34b9bb44 2023-05-15T15:09:45+02:00 Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. Regis Gomes Katrine Cavalcanti Clarissa Teixeira Augusto M Carvalho Paulo S Mattos Juqueline R Cristal Aline C Muniz José Carlos Miranda Camila I de Oliveira Aldina Barral 2016-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005078 https://doaj.org/article/c000fe0595114c408484917f34b9bb44 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5094744?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005078 https://doaj.org/article/c000fe0595114c408484917f34b9bb44 PLoS Neglected Tropical Diseases, Vol 10, Iss 11, p e0005078 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005078 2022-12-31T01:48:57Z Previous works showed that immunization with saliva from Lutzomyia intermedia, a vector of Leishmania braziliensis, does not protect against experimental infection. However, L. braziliensis is also transmitted by Lutzomyia whitmani, a sand fly species closely related to Lu. intermedia. Herein we describe the immune response following immunization with Lu. whitmani saliva and the outcome of this response after L. braziliensis infection.BALB/c mice immunized with Lu. whitmani saliva developed robust humoral and cellular immune responses, the latter characterized by an intense cellular infiltrate and production of IFN-γ and IL-10, by both CD4+ and CD8+ cells. Mice immunized as above and challenged with L. braziliensis plus Lu. whitmani saliva displayed significantly smaller lesions and parasite load at the challenge site. This protection was associated with a higher (p<0.05) IFN-γ production in response to SLA stimulation. Long-term persisting immunity was also detected in mice immunized with Lu. whitmani saliva. Furthermore, individuals residing in an endemic area for cutaneous leishmaniasis (CL) presented antibody responses to Lu. whitmani saliva. However CL patients, with active lesions, displayed a lower humoral response to Lu. whitmani saliva compared to individuals with subclinical Leishmania infection.Pre-exposure to Lu. whitmani saliva induces protection against L. braziliensis in a murine model. We also show that Lu. whitmani salivary proteins are immunogenic in naturally exposed individuals. Our results reinforce the importance of investigating the immunomodulatory effect of saliva from different species of closely related sand flies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 11 e0005078 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Regis Gomes Katrine Cavalcanti Clarissa Teixeira Augusto M Carvalho Paulo S Mattos Juqueline R Cristal Aline C Muniz José Carlos Miranda Camila I de Oliveira Aldina Barral Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Previous works showed that immunization with saliva from Lutzomyia intermedia, a vector of Leishmania braziliensis, does not protect against experimental infection. However, L. braziliensis is also transmitted by Lutzomyia whitmani, a sand fly species closely related to Lu. intermedia. Herein we describe the immune response following immunization with Lu. whitmani saliva and the outcome of this response after L. braziliensis infection.BALB/c mice immunized with Lu. whitmani saliva developed robust humoral and cellular immune responses, the latter characterized by an intense cellular infiltrate and production of IFN-γ and IL-10, by both CD4+ and CD8+ cells. Mice immunized as above and challenged with L. braziliensis plus Lu. whitmani saliva displayed significantly smaller lesions and parasite load at the challenge site. This protection was associated with a higher (p<0.05) IFN-γ production in response to SLA stimulation. Long-term persisting immunity was also detected in mice immunized with Lu. whitmani saliva. Furthermore, individuals residing in an endemic area for cutaneous leishmaniasis (CL) presented antibody responses to Lu. whitmani saliva. However CL patients, with active lesions, displayed a lower humoral response to Lu. whitmani saliva compared to individuals with subclinical Leishmania infection.Pre-exposure to Lu. whitmani saliva induces protection against L. braziliensis in a murine model. We also show that Lu. whitmani salivary proteins are immunogenic in naturally exposed individuals. Our results reinforce the importance of investigating the immunomodulatory effect of saliva from different species of closely related sand flies. |
format |
Article in Journal/Newspaper |
author |
Regis Gomes Katrine Cavalcanti Clarissa Teixeira Augusto M Carvalho Paulo S Mattos Juqueline R Cristal Aline C Muniz José Carlos Miranda Camila I de Oliveira Aldina Barral |
author_facet |
Regis Gomes Katrine Cavalcanti Clarissa Teixeira Augusto M Carvalho Paulo S Mattos Juqueline R Cristal Aline C Muniz José Carlos Miranda Camila I de Oliveira Aldina Barral |
author_sort |
Regis Gomes |
title |
Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. |
title_short |
Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. |
title_full |
Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. |
title_fullStr |
Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. |
title_full_unstemmed |
Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. |
title_sort |
immunity to lutzomyia whitmani saliva protects against experimental leishmania braziliensis infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0005078 https://doaj.org/article/c000fe0595114c408484917f34b9bb44 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 11, p e0005078 (2016) |
op_relation |
http://europepmc.org/articles/PMC5094744?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005078 https://doaj.org/article/c000fe0595114c408484917f34b9bb44 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005078 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
11 |
container_start_page |
e0005078 |
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1766340873476374528 |