Reversible Immobilization of Lipases on Heterofunctional Octyl-Amino Agarose Beads Prevents Enzyme Desorption

• Published in: Molecules
• Main Authors: Nazzoly Rueda, Tiago L. Albuquerque, Rocio Bartolome-Cabrero, Laura Fernandez-Lopez, Rodrigo Torres, Claudia Ortiz, Jose C. S. dos Santos, Oveimar Barbosa, Roberto Fernandez-Lafuente
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2016
• Subjects: heterofunctional supports; octyl supports; interfacial activation of lipases; ion exchange; enzyme hyperactivation; reversible immobilization; Organic chemistry; QD241-441; Rugosa; Triton; Antarc*; Antarctica
• Online Access: https://doi.org/10.3390/molecules21050646; https://doaj.org/article/bfea60a3028d40cd8cfce0e2409a32f6

Two different heterofunctional octyl-amino supports have been prepared using ethylenediamine and hexylendiamine (OCEDA and OCHDA) and utilized to immobilize five lipases (lipases A (CALA) and B (CALB) from Candida antarctica, lipases from Thermomyces lanuginosus (TLL), from Rhizomucor miehei (RML) a...