Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney
A new cell line derived from the kidney of European eel, Anguilla anguilla, has been established and characterized. This cell line, designated as EK (eel kidney), has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 ti...
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The Company of Biologists
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ftdoajarticles:oai:doaj.org/article:bfdcfc655711441cb1f78b795592088e 2023-05-15T13:26:50+02:00 Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney Bin Chen Zaiyu Zheng Jinxian Yang Hongshu Chi He Huang Hui Gong 2019-01-01T00:00:00Z https://doi.org/10.1242/bio.037507 https://doaj.org/article/bfdcfc655711441cb1f78b795592088e EN eng The Company of Biologists http://bio.biologists.org/content/8/1/bio037507 https://doaj.org/toc/2046-6390 2046-6390 doi:10.1242/bio.037507 https://doaj.org/article/bfdcfc655711441cb1f78b795592088e Biology Open, Vol 8, Iss 1 (2019) Anguilla anguilla Kidney cell line Rana grylio virus Immune-related gene Science Q Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.1242/bio.037507 2022-12-31T10:09:03Z A new cell line derived from the kidney of European eel, Anguilla anguilla, has been established and characterized. This cell line, designated as EK (eel kidney), has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 times. This cell line consists predominantly of fibroblast-like cells, and can grow at 15–37°C under an optimum temperature of 26°C. The origin of this cell line was confirmed by polymerase chain reaction (PCR) amplification and 18s recombinant (r)RNA sequencing. The chromosome analysis of EK cells at passage 58 revealed an ananeuploid karyotype. The EK cells were successfully transfected with the Pegfp-N1 plasmid, suggesting its potential in genetic studies. The susceptibility test showed a significant cytopathic effect (CPE) in EK cells for Rana grylio virus, and the viral replication was evidenced with quantitative real-time PCR (qRT-PCR) assay. After poly (I:C) stimulation, the expression of the immune-related molecules including interferon regulatory factor-3 (irf3), interferon regulatory factor-7 (irf7) and cytochrome P450 (CYP450) were significantly upregulated in EK cells, while the expression of transforming growth factor (TGF-β) was downregulated. These results suggested the potential of EK cell line as a model in gene engineering, virus identification and environmental toxicology. Article in Journal/Newspaper Anguilla anguilla Directory of Open Access Journals: DOAJ Articles Biology Open |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Anguilla anguilla Kidney cell line Rana grylio virus Immune-related gene Science Q Biology (General) QH301-705.5 |
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Anguilla anguilla Kidney cell line Rana grylio virus Immune-related gene Science Q Biology (General) QH301-705.5 Bin Chen Zaiyu Zheng Jinxian Yang Hongshu Chi He Huang Hui Gong Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
topic_facet |
Anguilla anguilla Kidney cell line Rana grylio virus Immune-related gene Science Q Biology (General) QH301-705.5 |
description |
A new cell line derived from the kidney of European eel, Anguilla anguilla, has been established and characterized. This cell line, designated as EK (eel kidney), has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 times. This cell line consists predominantly of fibroblast-like cells, and can grow at 15–37°C under an optimum temperature of 26°C. The origin of this cell line was confirmed by polymerase chain reaction (PCR) amplification and 18s recombinant (r)RNA sequencing. The chromosome analysis of EK cells at passage 58 revealed an ananeuploid karyotype. The EK cells were successfully transfected with the Pegfp-N1 plasmid, suggesting its potential in genetic studies. The susceptibility test showed a significant cytopathic effect (CPE) in EK cells for Rana grylio virus, and the viral replication was evidenced with quantitative real-time PCR (qRT-PCR) assay. After poly (I:C) stimulation, the expression of the immune-related molecules including interferon regulatory factor-3 (irf3), interferon regulatory factor-7 (irf7) and cytochrome P450 (CYP450) were significantly upregulated in EK cells, while the expression of transforming growth factor (TGF-β) was downregulated. These results suggested the potential of EK cell line as a model in gene engineering, virus identification and environmental toxicology. |
format |
Article in Journal/Newspaper |
author |
Bin Chen Zaiyu Zheng Jinxian Yang Hongshu Chi He Huang Hui Gong |
author_facet |
Bin Chen Zaiyu Zheng Jinxian Yang Hongshu Chi He Huang Hui Gong |
author_sort |
Bin Chen |
title |
Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
title_short |
Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
title_full |
Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
title_fullStr |
Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
title_full_unstemmed |
Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
title_sort |
development and characterization of a new cell line derived from european eel anguilla anguilla kidney |
publisher |
The Company of Biologists |
publishDate |
2019 |
url |
https://doi.org/10.1242/bio.037507 https://doaj.org/article/bfdcfc655711441cb1f78b795592088e |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Biology Open, Vol 8, Iss 1 (2019) |
op_relation |
http://bio.biologists.org/content/8/1/bio037507 https://doaj.org/toc/2046-6390 2046-6390 doi:10.1242/bio.037507 https://doaj.org/article/bfdcfc655711441cb1f78b795592088e |
op_doi |
https://doi.org/10.1242/bio.037507 |
container_title |
Biology Open |
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1766394727785037824 |