Biomarkers in Trypanosoma cruzi-infected and uninfected individuals with varying severity of cardiomyopathy in Santa Cruz, Bolivia.
Twenty to thirty percent of persons with Trypanosoma cruzi infection eventually develop cardiomyopathy. If an early indicator were to be identified and validated in longitudinal studies, this could enable treatment to be prioritized for those at highest risk. We evaluated cardiac and extracellular m...
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Online Access: | https://doi.org/10.1371/journal.pntd.0003227 https://doaj.org/article/bf924c187c2b403f82c6c4a5cecc20cf |
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ftdoajarticles:oai:doaj.org/article:bf924c187c2b403f82c6c4a5cecc20cf 2023-05-15T15:12:06+02:00 Biomarkers in Trypanosoma cruzi-infected and uninfected individuals with varying severity of cardiomyopathy in Santa Cruz, Bolivia. Emi E Okamoto Jacqueline E Sherbuk Eva H Clark Morgan A Marks Omar Gandarilla Gerson Galdos-Cardenas Angel Vasquez-Villar Jeong Choi Thomas C Crawford Rose Q Do Antonio B Fernandez Rony Colanzi Jorge Luis Flores-Franco Robert H Gilman Caryn Bern Chagas Disease Working Group in Bolivia and Peru 2014-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003227 https://doaj.org/article/bf924c187c2b403f82c6c4a5cecc20cf EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4183477?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003227 https://doaj.org/article/bf924c187c2b403f82c6c4a5cecc20cf PLoS Neglected Tropical Diseases, Vol 8, Iss 10, p e3227 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003227 2022-12-31T09:35:55Z Twenty to thirty percent of persons with Trypanosoma cruzi infection eventually develop cardiomyopathy. If an early indicator were to be identified and validated in longitudinal studies, this could enable treatment to be prioritized for those at highest risk. We evaluated cardiac and extracellular matrix remodeling markers across cardiac stages in T. cruzi infected (Tc+) and uninfected (Tc-) individuals.Participants were recruited in a public hospital in Santa Cruz, Bolivia and assigned cardiac severity stages by electrocardiogram and echocardiogram. BNP, NTproBNP, CKMB, troponin I, MMP-2, MMP-9, TIMP-1, TIMP-2, TGFb1, and TGFb2 were measured in specimens from 265 individuals using multiplex bead systems. Biomarker levels were compared between Tc+ and Tc- groups, and across cardiac stages. Receivers operating characteristic (ROC) curves were created; for markers with area under curve>0.60, logistic regression was performed.Analyses stratified by cardiac stage showed no significant differences in biomarker levels by Tc infection status. Among Tc+ individuals, those with cardiac insufficiency had higher levels of BNP, NTproBNP, troponin I, MMP-2, TIMP-1, and TIMP-2 than those with normal ejection fraction and left ventricular diameter. No individual marker distinguished between the two earliest Tc+ stages, but in ROC-based analyses, MMP-2/MMP-9 ratio was significantly higher in those with than those without ECG abnormalities.BNP, NTproBNP, troponin I, MMP-2, TIMP-1, and TIMP-2 levels rose with increasing severity stage but did not distinguish between Chagas cardiomyopathy and other cardiomyopathies. Among Tc+ individuals without cardiac insufficiency, only the MMP-2/MMP-9 ratio differed between those with and without ECG changes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 10 e3227 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Emi E Okamoto Jacqueline E Sherbuk Eva H Clark Morgan A Marks Omar Gandarilla Gerson Galdos-Cardenas Angel Vasquez-Villar Jeong Choi Thomas C Crawford Rose Q Do Antonio B Fernandez Rony Colanzi Jorge Luis Flores-Franco Robert H Gilman Caryn Bern Chagas Disease Working Group in Bolivia and Peru Biomarkers in Trypanosoma cruzi-infected and uninfected individuals with varying severity of cardiomyopathy in Santa Cruz, Bolivia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Twenty to thirty percent of persons with Trypanosoma cruzi infection eventually develop cardiomyopathy. If an early indicator were to be identified and validated in longitudinal studies, this could enable treatment to be prioritized for those at highest risk. We evaluated cardiac and extracellular matrix remodeling markers across cardiac stages in T. cruzi infected (Tc+) and uninfected (Tc-) individuals.Participants were recruited in a public hospital in Santa Cruz, Bolivia and assigned cardiac severity stages by electrocardiogram and echocardiogram. BNP, NTproBNP, CKMB, troponin I, MMP-2, MMP-9, TIMP-1, TIMP-2, TGFb1, and TGFb2 were measured in specimens from 265 individuals using multiplex bead systems. Biomarker levels were compared between Tc+ and Tc- groups, and across cardiac stages. Receivers operating characteristic (ROC) curves were created; for markers with area under curve>0.60, logistic regression was performed.Analyses stratified by cardiac stage showed no significant differences in biomarker levels by Tc infection status. Among Tc+ individuals, those with cardiac insufficiency had higher levels of BNP, NTproBNP, troponin I, MMP-2, TIMP-1, and TIMP-2 than those with normal ejection fraction and left ventricular diameter. No individual marker distinguished between the two earliest Tc+ stages, but in ROC-based analyses, MMP-2/MMP-9 ratio was significantly higher in those with than those without ECG abnormalities.BNP, NTproBNP, troponin I, MMP-2, TIMP-1, and TIMP-2 levels rose with increasing severity stage but did not distinguish between Chagas cardiomyopathy and other cardiomyopathies. Among Tc+ individuals without cardiac insufficiency, only the MMP-2/MMP-9 ratio differed between those with and without ECG changes. |
format |
Article in Journal/Newspaper |
author |
Emi E Okamoto Jacqueline E Sherbuk Eva H Clark Morgan A Marks Omar Gandarilla Gerson Galdos-Cardenas Angel Vasquez-Villar Jeong Choi Thomas C Crawford Rose Q Do Antonio B Fernandez Rony Colanzi Jorge Luis Flores-Franco Robert H Gilman Caryn Bern Chagas Disease Working Group in Bolivia and Peru |
author_facet |
Emi E Okamoto Jacqueline E Sherbuk Eva H Clark Morgan A Marks Omar Gandarilla Gerson Galdos-Cardenas Angel Vasquez-Villar Jeong Choi Thomas C Crawford Rose Q Do Antonio B Fernandez Rony Colanzi Jorge Luis Flores-Franco Robert H Gilman Caryn Bern Chagas Disease Working Group in Bolivia and Peru |
author_sort |
Emi E Okamoto |
title |
Biomarkers in Trypanosoma cruzi-infected and uninfected individuals with varying severity of cardiomyopathy in Santa Cruz, Bolivia. |
title_short |
Biomarkers in Trypanosoma cruzi-infected and uninfected individuals with varying severity of cardiomyopathy in Santa Cruz, Bolivia. |
title_full |
Biomarkers in Trypanosoma cruzi-infected and uninfected individuals with varying severity of cardiomyopathy in Santa Cruz, Bolivia. |
title_fullStr |
Biomarkers in Trypanosoma cruzi-infected and uninfected individuals with varying severity of cardiomyopathy in Santa Cruz, Bolivia. |
title_full_unstemmed |
Biomarkers in Trypanosoma cruzi-infected and uninfected individuals with varying severity of cardiomyopathy in Santa Cruz, Bolivia. |
title_sort |
biomarkers in trypanosoma cruzi-infected and uninfected individuals with varying severity of cardiomyopathy in santa cruz, bolivia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003227 https://doaj.org/article/bf924c187c2b403f82c6c4a5cecc20cf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 10, p e3227 (2014) |
op_relation |
http://europepmc.org/articles/PMC4183477?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003227 https://doaj.org/article/bf924c187c2b403f82c6c4a5cecc20cf |
op_doi |
https://doi.org/10.1371/journal.pntd.0003227 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
10 |
container_start_page |
e3227 |
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1766342841369362432 |