Genetic changes associated with the temporal shift in invasive non-typhoidal Salmonella serovars in Bamako Mali.
Background Invasive non-typhoidal Salmonella (iNTS) serovars S. Typhimurium and S. Enteritidis are major etiologic agents of invasive bacterial disease among infants and young children in sub-Saharan Africa, including in Mali. Early studies of iNTS serovars in several countries indicated that S. Typ...
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ftdoajarticles:oai:doaj.org/article:bf85513ebb29450cb1cfaa999dd95da9 2024-09-09T19:26:54+00:00 Genetic changes associated with the temporal shift in invasive non-typhoidal Salmonella serovars in Bamako Mali. Kristin Bornstein Sharon M Tennant Tracy H Hazen John D Sorkin Milagritos D Tapia Samba O Sow Uma Onwuchekwa Myron M Levine David A Rasko 2019-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007297 https://doaj.org/article/bf85513ebb29450cb1cfaa999dd95da9 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007297 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007297 https://doaj.org/article/bf85513ebb29450cb1cfaa999dd95da9 PLoS Neglected Tropical Diseases, Vol 13, Iss 6, p e0007297 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007297 2024-08-05T17:48:54Z Background Invasive non-typhoidal Salmonella (iNTS) serovars S. Typhimurium and S. Enteritidis are major etiologic agents of invasive bacterial disease among infants and young children in sub-Saharan Africa, including in Mali. Early studies of iNTS serovars in several countries indicated that S. Typhimurium was more prevalent than S. Enteritidis, including in Mali before 2008. We investigated genomic and associated phenotypic changes associated with an increase in the relative proportion of iNTS caused by S. Enteritidis versus S. Typhimurium in Bamako, Mali, during the period 2002-2012. Methodology/principal findings Comparative genomics studies identified homologs of tetracycline resistance and arsenic utilization genes that were associated with the temporal shift of serovars causing iNTS shift, along with several hypothetical proteins. These findings, validated through PCR screening and phenotypic assays, provide initial steps towards characterizing the genomic changes consequent to unknown evolutionary pressures associated with the shift in serovar prevalence. Conclusions/significance This work identified a shift to S. Enteritidis from the more classic S. Typhimurium, associated with iNTS in Bamako, Mali, during the period 2002-2012. This type of shift in underlying iNTS pathogens are of great importance to pediatric public health in endemic regions of sub-Saharan Africa. Additionally, this work demonstrates the utility of combining epidemiologic data, whole genome sequencing, and functional characterization in the laboratory to identify and characterize genomic changes in the isolates that may be involved with the observed shift in circulating iNTS agents. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 6 e0007297 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kristin Bornstein Sharon M Tennant Tracy H Hazen John D Sorkin Milagritos D Tapia Samba O Sow Uma Onwuchekwa Myron M Levine David A Rasko Genetic changes associated with the temporal shift in invasive non-typhoidal Salmonella serovars in Bamako Mali. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Invasive non-typhoidal Salmonella (iNTS) serovars S. Typhimurium and S. Enteritidis are major etiologic agents of invasive bacterial disease among infants and young children in sub-Saharan Africa, including in Mali. Early studies of iNTS serovars in several countries indicated that S. Typhimurium was more prevalent than S. Enteritidis, including in Mali before 2008. We investigated genomic and associated phenotypic changes associated with an increase in the relative proportion of iNTS caused by S. Enteritidis versus S. Typhimurium in Bamako, Mali, during the period 2002-2012. Methodology/principal findings Comparative genomics studies identified homologs of tetracycline resistance and arsenic utilization genes that were associated with the temporal shift of serovars causing iNTS shift, along with several hypothetical proteins. These findings, validated through PCR screening and phenotypic assays, provide initial steps towards characterizing the genomic changes consequent to unknown evolutionary pressures associated with the shift in serovar prevalence. Conclusions/significance This work identified a shift to S. Enteritidis from the more classic S. Typhimurium, associated with iNTS in Bamako, Mali, during the period 2002-2012. This type of shift in underlying iNTS pathogens are of great importance to pediatric public health in endemic regions of sub-Saharan Africa. Additionally, this work demonstrates the utility of combining epidemiologic data, whole genome sequencing, and functional characterization in the laboratory to identify and characterize genomic changes in the isolates that may be involved with the observed shift in circulating iNTS agents. |
format |
Article in Journal/Newspaper |
author |
Kristin Bornstein Sharon M Tennant Tracy H Hazen John D Sorkin Milagritos D Tapia Samba O Sow Uma Onwuchekwa Myron M Levine David A Rasko |
author_facet |
Kristin Bornstein Sharon M Tennant Tracy H Hazen John D Sorkin Milagritos D Tapia Samba O Sow Uma Onwuchekwa Myron M Levine David A Rasko |
author_sort |
Kristin Bornstein |
title |
Genetic changes associated with the temporal shift in invasive non-typhoidal Salmonella serovars in Bamako Mali. |
title_short |
Genetic changes associated with the temporal shift in invasive non-typhoidal Salmonella serovars in Bamako Mali. |
title_full |
Genetic changes associated with the temporal shift in invasive non-typhoidal Salmonella serovars in Bamako Mali. |
title_fullStr |
Genetic changes associated with the temporal shift in invasive non-typhoidal Salmonella serovars in Bamako Mali. |
title_full_unstemmed |
Genetic changes associated with the temporal shift in invasive non-typhoidal Salmonella serovars in Bamako Mali. |
title_sort |
genetic changes associated with the temporal shift in invasive non-typhoidal salmonella serovars in bamako mali. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007297 https://doaj.org/article/bf85513ebb29450cb1cfaa999dd95da9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 6, p e0007297 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007297 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007297 https://doaj.org/article/bf85513ebb29450cb1cfaa999dd95da9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007297 |
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PLOS Neglected Tropical Diseases |
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13 |
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6 |
container_start_page |
e0007297 |
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1809896437421441024 |