Effect of glutamine supplementation against soybean meal-induced growth retardation, hepatic metabolomics and transcriptome alterations in hybrid sturgeon Acipenser baerii♀× A. schrenckii ♂
This study aimed to evaluate the effects of glutamine supplementation to soybean meal (SBM) diet in hybrid sturgeon (25.00 ± 1.00 g). Three diets, including fishmeal (FM) diet, SBM diet, and SBMG diet (glutamine addition to SBM diet), were formulated to feed sturgeons for 8 weeks. Growth parameters...
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ftdoajarticles:oai:doaj.org/article:bf53692be4f14de29ab1aeb47ee3aefc 2023-05-15T13:01:53+02:00 Effect of glutamine supplementation against soybean meal-induced growth retardation, hepatic metabolomics and transcriptome alterations in hybrid sturgeon Acipenser baerii♀× A. schrenckii ♂ Huamei Yue Jinping Wu Peng Fu Rui Ruan Huan Ye Bin Hu Xihua Chen Chuangju Li 2022-06-01T00:00:00Z https://doi.org/10.1016/j.aqrep.2022.101158 https://doaj.org/article/bf53692be4f14de29ab1aeb47ee3aefc EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2352513422001545 https://doaj.org/toc/2352-5134 2352-5134 doi:10.1016/j.aqrep.2022.101158 https://doaj.org/article/bf53692be4f14de29ab1aeb47ee3aefc Aquaculture Reports, Vol 24, Iss , Pp 101158- (2022) Soybean meal Glutamine Liver morphology Metabolomics Transcriptome Aquaculture. Fisheries. Angling SH1-691 article 2022 ftdoajarticles https://doi.org/10.1016/j.aqrep.2022.101158 2022-12-31T03:05:50Z This study aimed to evaluate the effects of glutamine supplementation to soybean meal (SBM) diet in hybrid sturgeon (25.00 ± 1.00 g). Three diets, including fishmeal (FM) diet, SBM diet, and SBMG diet (glutamine addition to SBM diet), were formulated to feed sturgeons for 8 weeks. Growth parameters and muscular crude lipid content were reduced by SBM substitution, and were further increased in SBMG-diet group. In liver morphology detection, moderate lipid droplets accumulation in hepatocytes found in fish fed SBM diet were partially retrieved by glutamine addition. Hepatic gas chromatography–mass spectrometry analysis detected 156 up-regulated metabolites and 15 related KEGG pathways affected by SBM replacement. Glutamine addition resulted in 102 down-regulated metabolites, together with 9 KEGG pathways enriched. In liver transcriptome sequencing analysis, 1074 and 1564 differentially expressed unigenes (DEGs) were detected by SBM/FM and SBMG/SBM pairwise comparisons, respectively. The least number of DEGs (143) was present in pairwise comparison of SBMG /FM. Our results indicated that glutamine addition attenuated the SBM-based negative effect including growth retardation, and muscle proximate composition alteration. Glutamine supplementation alleviated the liver damage caused by SBM substitution possibly through down regulating the elevated levels of metabolites or gene as well as related metabolic pathways. Article in Journal/Newspaper Acipenser baerii Directory of Open Access Journals: DOAJ Articles Aquaculture Reports 24 101158 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Soybean meal Glutamine Liver morphology Metabolomics Transcriptome Aquaculture. Fisheries. Angling SH1-691 |
spellingShingle |
Soybean meal Glutamine Liver morphology Metabolomics Transcriptome Aquaculture. Fisheries. Angling SH1-691 Huamei Yue Jinping Wu Peng Fu Rui Ruan Huan Ye Bin Hu Xihua Chen Chuangju Li Effect of glutamine supplementation against soybean meal-induced growth retardation, hepatic metabolomics and transcriptome alterations in hybrid sturgeon Acipenser baerii♀× A. schrenckii ♂ |
topic_facet |
Soybean meal Glutamine Liver morphology Metabolomics Transcriptome Aquaculture. Fisheries. Angling SH1-691 |
description |
This study aimed to evaluate the effects of glutamine supplementation to soybean meal (SBM) diet in hybrid sturgeon (25.00 ± 1.00 g). Three diets, including fishmeal (FM) diet, SBM diet, and SBMG diet (glutamine addition to SBM diet), were formulated to feed sturgeons for 8 weeks. Growth parameters and muscular crude lipid content were reduced by SBM substitution, and were further increased in SBMG-diet group. In liver morphology detection, moderate lipid droplets accumulation in hepatocytes found in fish fed SBM diet were partially retrieved by glutamine addition. Hepatic gas chromatography–mass spectrometry analysis detected 156 up-regulated metabolites and 15 related KEGG pathways affected by SBM replacement. Glutamine addition resulted in 102 down-regulated metabolites, together with 9 KEGG pathways enriched. In liver transcriptome sequencing analysis, 1074 and 1564 differentially expressed unigenes (DEGs) were detected by SBM/FM and SBMG/SBM pairwise comparisons, respectively. The least number of DEGs (143) was present in pairwise comparison of SBMG /FM. Our results indicated that glutamine addition attenuated the SBM-based negative effect including growth retardation, and muscle proximate composition alteration. Glutamine supplementation alleviated the liver damage caused by SBM substitution possibly through down regulating the elevated levels of metabolites or gene as well as related metabolic pathways. |
format |
Article in Journal/Newspaper |
author |
Huamei Yue Jinping Wu Peng Fu Rui Ruan Huan Ye Bin Hu Xihua Chen Chuangju Li |
author_facet |
Huamei Yue Jinping Wu Peng Fu Rui Ruan Huan Ye Bin Hu Xihua Chen Chuangju Li |
author_sort |
Huamei Yue |
title |
Effect of glutamine supplementation against soybean meal-induced growth retardation, hepatic metabolomics and transcriptome alterations in hybrid sturgeon Acipenser baerii♀× A. schrenckii ♂ |
title_short |
Effect of glutamine supplementation against soybean meal-induced growth retardation, hepatic metabolomics and transcriptome alterations in hybrid sturgeon Acipenser baerii♀× A. schrenckii ♂ |
title_full |
Effect of glutamine supplementation against soybean meal-induced growth retardation, hepatic metabolomics and transcriptome alterations in hybrid sturgeon Acipenser baerii♀× A. schrenckii ♂ |
title_fullStr |
Effect of glutamine supplementation against soybean meal-induced growth retardation, hepatic metabolomics and transcriptome alterations in hybrid sturgeon Acipenser baerii♀× A. schrenckii ♂ |
title_full_unstemmed |
Effect of glutamine supplementation against soybean meal-induced growth retardation, hepatic metabolomics and transcriptome alterations in hybrid sturgeon Acipenser baerii♀× A. schrenckii ♂ |
title_sort |
effect of glutamine supplementation against soybean meal-induced growth retardation, hepatic metabolomics and transcriptome alterations in hybrid sturgeon acipenser baerii♀× a. schrenckii ♂ |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.aqrep.2022.101158 https://doaj.org/article/bf53692be4f14de29ab1aeb47ee3aefc |
genre |
Acipenser baerii |
genre_facet |
Acipenser baerii |
op_source |
Aquaculture Reports, Vol 24, Iss , Pp 101158- (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2352513422001545 https://doaj.org/toc/2352-5134 2352-5134 doi:10.1016/j.aqrep.2022.101158 https://doaj.org/article/bf53692be4f14de29ab1aeb47ee3aefc |
op_doi |
https://doi.org/10.1016/j.aqrep.2022.101158 |
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Aquaculture Reports |
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24 |
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101158 |
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1766288978506416128 |